Published: 2017-08-26

Blood group and its relationship with bleeding time and clotting time- an observational study among the 1st MBBS students of Gauhati medical college, Guwahati

Reeta Baishya, Raj Sarkar, Barasha Barman



Background: Blood group and its relation to bleeding time (BT) and clotting time (CT) is important in conditions like epistaxis, thrombosis, and surgery. Earlier studies depicted O group having prolonged BT and CT. The aim of this study was to assess the relationship of BT and CT with ABO groups.

Methods: This cross-sectional study was conducted in the department of physiology, Gauhati medical college. The study included 154 students. Blood grouping was determined with the standard antiserum; BT and CT were estimated by the duke method and slide method, respectively. Blood group and its relation to BT, CT were analyzed by Chi-square analysis.

Results: Blood group O (42.2%) was predominant in both genders followed by B (31.8%), A (21%), AB (4.5%). CT was found to be more than 6 minutes in group O (31.57%) followed by group AB (26.31%), A and B (21.05%). The difference was statistically significant (p = 0.02). BT was found to be more than 4 minutes in group O (82.35%) followed by A (11.76%), B (5.88%), and AB (0%). That was statistically significant (p = 0.01). CT was more than 6 minutes in 57.89% in females as compared to 42.10% in males, variation was statistically significant (p = 0.01). BT was more than 4 minutes in 58.82% females as compared to 41.17% in males. The variation was statistically significant (p = 0.03).

Conclusions: In present study blood group O was more common followed by B, A, and AB. CT and BT were prolonged in O group. BT and CT were more in females than males.


Blood group, Bleeding time, Clotting time

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Watkins WM. Biochemistry and genetics of ABO, Lewis and P blood group systems. Adv Hum Genet. 1980;10:1-36.

Akhtar MN, Tayyib A, Tasneem T, Butt AR. ABO blood group in patients with peptic ulcer disease: Association with secretor status. Ann King Edward Med Coll. 2003;9:238-40.

Qureshi MA, Bhatti R. Frequency of ABO blood groups among the diabetes mellitus type 2 patients. J Coll Physicians Surg Pak. 2003;13:453-5.

Ziegler T, Jacobsohn N, Funfstuck R. Correlation between blood group phenotype and virulence properties of Escherichia coli in patients with chronic urinary tract infection. Int J Antimicrob Agents. 2004;24(1):S70-5.

Mourant AE. Blood relations: Blood groups and anthropology. Oxford: Oxford University Press. 1983:1-146.

Favaloro EJ, Soltani S, McDonald J, Grezchnik E, Easton L, Favaloro JW. Reassessment of ABO blood group, sex, and age on laboratory parameters used to diagnose von Willebrand disorder: potential influence on the diagnosis vs the potential association with risk of thrombosis Am J Clin Pathol. 2005; 124(6):910-7.

Ruggeri ZM, Zimmerman TS. The complex multimeric composition of factor VIII/vWF. Blood. 1981;57:1140-3.

Sadler JE. Von Willebrand factor. Ann Rev Biochem. 1998,67:395-424.

Ruggeri ZM. Structure of von Willebrand factor and its functioning platelet adhesion and thrombus formation. Best Prac Res Clin Haematol. 2001;14:257-9.

Gill JC, Endres-Brooks J, Bauer PJ, Marks WJ, Montgomery RR. The effect of ABO blood group on the diagnosis of von Willebrand disease. Blood. 1987;69(6):1691-5.

Daniel M, Jaberoo MC, Stead RE, Reddy VM, Moir AA. Is admission for epistaxis more common in Caucasian than in Asian people? preliminary study. Clin Otolaryngol. 2006;31:386-9.

Reddy VM, Daniel M, Bright E, Broad SR, Moir AA. Is there an association between blood group O and epistaxis? J Laryngol Otol. 2008;122(4):366-8.

Mourant, A.E., 1983. Blood Relations: Blood Groups and Anthropology. Oxford University Press, New York; 1983:146.

Favaloro, E.J., S. Soltani, J. McDonald, E. Grezchnik, L. Easton and J.W. Favaloro, 2005. Reassesment of ABO blood group, sex and age on laboratory parameters used to diagnose Von Willebrand disorder: Potential influence on the diagnosis Vs the potential association with risk of thrombosis. Am J Clin Pathol. 2005;124:910-7.

Ercan M, Yegin E, Akdeniz H, Irmak H, Bayiroglu F, Tuncer I. Effect of oestrogen on fibrinogen clotting time in rabbits. Trans J Vet Anim Sci. 1998;22:137-40.