Published: 2016-12-19

An experimental study to evaluate and compare the analgesic activity of calcitriol with morphine in albino mice at a tertiary care teaching hospital in Maharashtra, India

Abhinav David, Raj Kumar Goel, Prashant Patel, Rahul Kunkolol, Dattatray H. Nandal


Background: Vitamin D plays vital role in physiological functions in humans through its active form Calcitriol (Vitamin D3). Vitamin D receptors are found in most tissues, attributing to its classic and non-classic actions. Calcitriol exerts important regulatory effects on the molecular pathways involved in inflammation and pain. The present study is done for experimental evaluation of analgesic action of Calcitriol using pain models in albino mice.

Methods: In this prospective, experimental study, healthy Swiss albino mice were taken after permission from IAEC. Mice were divided into five groups as Control- treated with normal saline, Standard- treated with morphine and Test groups- treated with Calcitriol in dose of 15 µg /kg/mice, 30 µg /kg/mice and 60 µg/kg/mice respectively. Evaluation of analgesic activity was done using Hot plate and Tail flick analgesiometer.

Results: All the 3 test doses of Calcitriol although showed prolongation of reaction time in Hot plate method up to 60 mins but the analgesic activity was not significant in comparison with the standard Morphine. With Tail flick method Calcitriol failed to show any analgesic efficacy at 15-30 μg/Kg but showed some analgesia at 60 μg/Kg which was more than control but not at all comparable with the standard Morphine for thermal pain.

Conclusions: The analgesic activity of Calcitriol was exhibited at higher doses. This property needs to be further evaluated by planning extensive animal experimentation using different animal models.


Calcitriol, Escape reaction, Hot plate, Non classic action, Paw withdrawal, Tail flick analgesiometer

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Adams JS, Hewison M. Update in Vitamin D. J Clin Endocrin Metab. 2010;95(2):471-8.

Holick MF. Vitamin D: the underappreciated D-lightful hormone that is important for skeletal and cellular health. Curr Opin Endocrinol Diabetes. 2002;9:87-98.

Grant WB. An estimate of premature cancer mortality in the U.S. due to inadequate doses of solar ultraviolet-B radiation. Cancer. 2002;94(6):1867-75.

Holick MF. Vitamin D: A millenium perspective. Journal of Cellular Biochemistry. 2003;88(2):296-307.

Holick MF. Vitamin D: photobiology, metabolism, mechanism of action, and clinical applications. In: Favus MJ, ed Primer on the Metabolic Bone Diseases and Disorders of Mineral Metabolism 5th ed Washington, DC: Amer Soc Bone Mineral Rese. 2003;5:129-37

Johnson LE. Vitamin D. Merck Manual [online] 2007 Available from: mmpe/ sec01/ ch004/ ch004k.html? qt=vitamin%20D&alt=sh#t b004_6. Accessed 2/19/08

Vasquez A, Manso G, Cannell J. The clinical importance of vitamin D (cholecalciferol): a paradigm shirt with implications for all healthcare providers. Alternative Therapies. 2004;10(5):28-36.

Bikle D. Nonclassic Actions of Vitamin D. J Clin Endocrin Metab. 2008;94(1):26-34.

Krishnan AV, Feldman D. Mechanisms of the Anti-Cancer and Anti-Inflammatory Actions of Vitamin D. Ann Rev Pharmaco Toxico. 2011;51(1):311-36.

Hudspith MJ, Siddall PJ, Munglani R. Physiology of pain. HEMMINGS, HC; HOPKINS, M Foundations of Anesthesia [Internet] 2006. Available from: downloads/ basic_sciences_articles/ pain-andanalgesia/ nociception.pdf.

Reagan-Shaw S, Nihal M, Ahmad, N. Dose translation from animal to human studies revisited. FASEB. 2007;22:659-61.

Institute of Medicine, Food and Nutrition Board. Dietary Reference Intakes for Calcium and Vitamin D. Washington, DC: National Academy Press. 2010.

Vogel HG. Drug discovery and evaluation : pharmacological assays / (ed.).-- 2nd ed. 2002:694-6.

Bikash M, Ajay P. Practical Manual of Experimental and Clinical Pharmacology. Edition 2010:201-2

Sopan BP, Kshitij RP, Anil CN, Sudhir A, Ananda PRB. Evaluation of Analgesic activity of Hibiscus tiliaceus Linn Wood. World J Pharma Pharmaceu Scie. 2012;1(1):290-7.

Leavitt SB. Vitamin D–A neglected “analgesic”for chronic musculoskeletal pain. Pain Treatment Topics [Internet]. 2008. Available from: links/ RSD/ vitamind-report.pdf.