Study of antibiotic resistance profiling of Staphylococcus aureus isolated from clinical specimens of the patients attending a tertiary teaching hospital from Chhattisgarh

Archana Bhimrao Wankhade, Sanjibkumar Panda, Riddhi Hathiwala, Yogendra Keche


Background: Staphylococcus aureus is a pathogen causing wide spectrum of infections. It has tendency for the development of multidrug resistance thereby exposing the selection of appropriate treatment. Therefore, the present study was undertaken to find out the antibiotic susceptibility pattern of Staphylococcus aureus isolated from various clinical samples in teaching tertiary hospital.

Methods: Total 85 Staphylococcus aureus was isolated from clinical samples (pus, urine, sputum and blood) tested. Identification of Staphylococcus aureus was done by standard conventional microbiological methods. Antibiotic susceptibility testing was done by using disk diffusion method as per CLSI guidelines.

Results: Staphylococcus aureus was isolated maximum from pus samples followed by urine samples. Antibiotic susceptibility testing showed highest resistance against Penicillin (69%) and Erythromycin (51%) followed by Cotrimoxazole (50%) & Nitrofurantoin (50%). All the strains were sensitive to Vancomycin. Amongst the urine isolates all were sensitive to Norfloxacin. These percentages of sensitivities are characteristically higher in our study than the previous studies in the literature. In addition, out of 85 Staphylococcus aureus isolates, 26 isolates showed sensitivity   to all antibiotics.

Conclusions: From the present study we conclude that though the Staphylococcus aureus is usually having multidrug resistance pattern. So regular antimicrobial susceptibility surveillance is essential for area‐wise monitoring of the resistance patterns. This will be beneficial to preserve the effectiveness of antibiotics and for better patient management.


Antibiotic resistance, MRSA, MSSA, Staphylococcus

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Boucher HW & Corey GR Epidemiology of Methicillin resistant Staphylococcus aureus.clinical infectious diseases 2008; 46:344-349.

Cheesbrough MC. (2006). District Laboratory Practice in Tropical Countries. 1st Edn, Cambridge University Press, Cambridge, UK.

B. Forbes, D. Sahm, and A. Weissfeld, “Staphylococcus, micrococcus and similar organisms,” in Bailey and Scott’s Diagnostic Microbiology, MOSBY Elsevier, 12th edition, 2007.

T. Delorme, S. Rose, J. Senita, C. Callahan, and P. Nasr, “Epidemiology and susceptibilities of methicillin-resistant Staphylococcus aureus in Northeastern Ohio,” The American Journal of Clinical Pathology, 2009;132(5):616-687.

Mazhar Salim Al-Zoubi, Ibrahim Ali Al-Tayyar,Emad Hussein, Alla Al Jabali,and Salih Khudairat Antimicrobial susceptibility pattern of Staphylococcus aureus isolated from clinical specimens in Northern area of Jordan .Iran J Microbiol. 2015 ; 7(5): 265–272.

Shittu AO, Lin J. Antimicrobial susceptibility patterns and characterization of clinical isolates of Staphylococcus aureus in KwaZulu-Natal province, South Africa. BMC Infect Dis. 2006 Jul 28;6:125.

D. H. Tambekar, D. V. Dhanorkar, S. R. Gulhane, and M. N. Dudhane, “Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus from healthcare and community associated sources,” African Journal of Infectious Disease, 2007;1: 52–56.

Hemamalini V, Kavitha V, Ramachandran S In vitro antibiogram pattern of Staphylococcus aureus isolated from wound infection and molecular analysis of mecA gene and restriction sites in methicillin resistant Staphylococcus aureus,J Adv Pharm Technol Res. 2015; 6(4): 170–175.

Rajaduraipandi K, Mani KR, Panneerselvam K, Mani M, Bhaskar M, Manikandan P. Prevalence and antimicrobial susceptibility pattern of methicillin resistant Staphylococcus aureus: a multicentre study. Indian I Med Microbiol 2006. 24: 34– 38.

A. P. Mehta, C. Rodrigues, K. Sheth, S. Jani, A. Hakimiyan, and N. Fazalboy, “Control of Methicillin Resistant S. aureus in tertiary care centre-a five year study,” Indian Journal of Medical Microbiology.1998;16(1):31-34.

Yadhav K, Gayathri J. PanickerPrevalence and antibiogram of methicillin resistant staphylococcus aureus isolates at a tertiary care hospital in Bangalore, South India .Int J Cur Res 2014; 6 (17) 37-40.

Chandrashekar D K et al. Prevalence of Methicillin Resistant Staphylococcus aureus in a tertiary care hospital in Gul¬barga, Karnataka, J. of Pharmaceutical and Biomedical Sci¬ences. 2012; 19(19):1-3.

Clinical and Laboratory Standerd Institute, performance standards of antimicrobial susceptibility testing, M-100-27th edition.Jan 2017, CLSI, Wayne, PA 19087 USA.

Kandle SK, Ghatole MP, Takpere AY, Hittinhalli VB, Yemul VL. Bacteriophage typing and antibiotic sensitivity pat¬tern of Staphylococcus aureus from clinical specimen in and around Solapur (South Maharashtra) J Commun Dis. 2003; 35:17–23.