DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20164563

HPV-DNA testing for detecting precancerous lesions of cervix: how effective?

Ruchi N. Thakur

Abstract


Background: Human Papilloma Virus has been found to be associated with cervical squamous cell carcinoma. Studies and reviews indicate that HPV testing is more sensitive than Pap smear for identifying cervical cancer and its precursors in population screening. Hybrid capture 2 assays detect high oncogenic risk viruses and can be used as primary screening tool for women older than 30 years.

Methods: A detailed history followed by a thorough clinical and gynecological examination was carried out for women attending the gynecological O.P.D. HPV-DNA (HCT) samples were collected using a cytobrush. Suspicious lesions of the cervix were further subjected to colposcopy directed loop electrosurgical excision procedure (LEEP) and sent for histopathological examination in formalin. The results of HPV-DNA (HCT) and histopatholgical report were then correlated.

Results: In this study, one hundred and sixty women were screened for HPV-DNA. Thirty-two women were found to be positive for HPV-DNA. They were further subjected to colposcopy directed large loop electrosurgical procedure. The positive patients were found to have either low-grade or high-grade cervical intraepithelial neoplasia.

Conclusions: The use of HPV-DNA test may make it a viable alternative to cytological screening especially as a less frequent screening.

Keywords


CIN, HPV DNA, Human papilloma virus

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References


Kulasingam SL, Hughes JB. Evaluation of human papilloma virus testing in primary screening for cervical abnormalities. Comparison of sensitivity; specificity and frequency of referral. JAMA. 2002;288:1749-57.

Cuzick J, Szarewski, AHPV testing in primary screening of older women. BrJ cancer. 1999;81:554-8.

Koutsky LA, Holmes KK, Critchlow CW. A cohort study of the risk of cervical intraepithelial neoplasia grade 2 or 3 in relation to papilloma virus infection. N Engl J Med. 1992;327(18):1272-8.

Schiffmann B. IARC. 2002.

Snijders PJ, Van der Brule AJ. The clinical relevance of HPV testing: Relationship between analytical and clinical sensitivity. J. Pathology. 2003;201(1):1-6.

PATH (Program for appropriate Technology in Health). Preventing cervical cancer in low resource setting outlook. 2000;18(1):1-8.

Parkin M. Personal communication. IARC. 2000

Sriamporn S, Khuhaprema T, Parkin M. Cervical cancer screening in Thailand: an overview. J Med Screen. 2006;13Suppl 1:S39-43.

Sankaranarayanan R, Nene BM, Osmanabad District Cervical Screening Study Group, et al. A cluster randomized controlled trial of visual, cytology and humanpapillomavirus screening for cancer of the cervix in rural India. Int J Cancer. 2005;116(4):617-23.