Evaluation of micro-ESR method with Westergren method for determination of erythrocyte sedimentation rate
DOI:
https://doi.org/10.18203/2320-6012.ijrms20180311Keywords:
ESR, Micro-ESR method, Westergren methodAbstract
Background: Erythrocyte sedimentation rate (ESR) test provides valuable information in screening, diagnosis, as well as monitoring disease activity and therapeutic response in numerous health conditions. The most commonly used method for determination of ESR is Westergren method, which is time-consuming and requires a large amount of blood sample. There are several other methods, like Micro-ESR method which overcome the limitations of conventional Westergren method, hence the present study was performed to compare results of Westergren method with Micro-ESR method for determination of ESR.
Methods: In the present study, blood samples from 100 patients were processed for ESR determination by Westergren method and Micro-ESR method. The results obtained were compared using Pearson’s correlation test.
Results: The Westergren method was the reference method and the Micro-ESR method was testing method. The comparison was done between Micro- ESR method results (X-axis) and results of Westergren method (Y-axis). The slope of the regression line using linear regression was 1.010 with a y-intercept of -0.788. Statistical analysis demonstrated significant correlation of results of Micro-ESR method with Westergren method (r = 0.9977).
Conclusions: Micro-ESR is a reliable and precise method for ESR measurement. The Micro- ESR method is simple to perform and requires very small volume of blood (0.2ml) as compared to conventional Westergren method. It can be potential useful tool in performing ESR determination especially for the patients with limitation of blood availability as pediatric patients and very old patients.
References
West BA, Tabansi PN, Ugwu RO, Eneh AU. The predictive value of Micro-erythrocyte sedimentation rate in neonatal sepsis in a low resource country. Pediatr Therapeut. 2012;S2:002.
Adhikari BC, Patra S, Chanda C, Shrivastava RK. Erythrocyte sedimentation rate, measurement by capillary tube method, (Micro-ESR)-best method for neonate and small children. J Med Sci Clin Res. 2017;05(05):22135-7.
Shelat SG, Chacosky D, Shibutani S. Differences in erythrocyte sedimentation rates using the Westergren method and a centrifugation method. Am J Clin Pathol. 2008;130:127-30.
Subramanian A, Rangarajan K, Pandey RM, Gandhi JS, Sharma V, Bhoi SK. Evaluation of an automated erythrocyte sedimentation rate analyzer as compared to the Westergren manual method in measurement of erythrocyte sedimentation rate. Indian J Pathol Microbiol. 2011;54(1)70-4.
Westergren A. The technique of the red cell sedimentation reaction. Am Rev Tuberc. 1926;14:94-101.
Sox HC Jr, Liang MH. The erythrocyte sedimentation rate: guidelines for rational use. Ann Intern Med. 1986;104:515-23.
Saadeh C. The erythrocyte sedimentation rate: old and new clinical applications. South Med J. 1998;91:220-5.
International Council for Standardization in Haematology Expert Panel on Blood Rheology. ICSH recommendations for measurement of erythrocyte sedimentation rate. J Clin Pathol. 1993;46:198-203.
National Committee for Clinical Laboratory Standards 2000. Reference and Selected Procedures for the Erythrocyte Sedimentation Rate (ESR) Test (H2-A4). Wayne, PA:NCCLS;2000.
Zhao TX, Lockner D. Electrical impedance and erythrocyte sedimentation rate (ESR) of blood. Biochim Biophys Acta. 1993;1153(2):243-8.
Dobashi T, Idonuma A, Toyama, Sakanishi A. Effect of concentration on enhanced sedimentation rate of erythrocytes in an inclined vessel. Biorheology. 1994;31:383-93.
Manley RW. The effect of room temperature on erythrocyte sedimentation rate and its correction. J Clin Pathol. 1957;10(4):354-6.
Wongsena W, Kunakorn M, Tanprasert S, Petchclai B. Detection of HIV-1 window period infection in blood donors using borderline anti-HIV results, HIV-1 proviral DNA PCR, and HIV-1 antigen test. J Med Assoc Thai. 1997;80Suppl1:S112-5.
Stuart J, Barrett BA, Prangnell DR. Capillary blood collection in haematology. J Clin Pathol. 1974;27:869-74.
Shah Y, Kumar A. Erythrocyte sedimentation rate: evaluation of a micro technique. J Natl Med Assoc. 1982;74(9):887-9.
Napitupulu A, Sembiring R, Lubis CP, Manoeroeng SM, Siregar H. The micro erythrocyte sedimentation rate (ESR) among children of the tobacco plantation laborers in North Sumatra. Paediatr Indones. 1979;19(11-12):302-7.
Barrett BA, Hill PI. A micromethod for the erythrocyte sedimentation rate suitable for use on venous or capillary blood. J Clin Pathol. 1980;33(11):1118-20.