Quantitative analysis of chemical constituents in medicinal plant coleus aromaticus extracts


  • Santhosh Kumar N. Department of Biochemistry, Shridevi Institute of Medical Sciences and Research Hospital, Tumkur, Karnataka, India
  • Aliya Nusrath Department of Biochemistry, Adichunchanagiri Institute of Medical Sciences, B. G. Nagara, Mandya, Karnataka, India
  • Dinesha Ramadas AIMS-Central Research Laboratory, Adichunchanagiri Institute for Molecular Medicine, B. G. Nagara, Mandya, Karnataka, India




Dialyzed Coleus aromaticus protein (DCAPE), Hydro alcoholic extract (HAE), Ethanol extract (EE) and Chloroform extract (CE)


Background: Indian medicinal plants have great potential towards curing many diseases. Medicinal plant Coleus aromaticus is known for its wide medical applications. The main objectives of the study undertaken were to analyze the phytochemicals and compare the concentration present in the dialyzed Coleus aromaticus protein extract with various solvents.

Methods: Various extracts of the medicinal plant Coleus aromaticus leaves such as dialyzed Coleus aromaticus protein, hydro alcoholic extract, ethanol extract and chloroform extract were prepared and analyzed for various phytochemical concentrations such as Total phenols (Folin-Ciocalteu method), Flavonoids (Aluminum chloride method), total sugars (Dubois method) and Proteins (Bradford’s method). Standard graph for each component was plotted.

Results: The protein concentration in dialyzed Coleus aromaticus protein extract is considerably higher (5.8μg/10μl) than hydro alcoholic extract (1.6μg/10μl), ethanol extract (5.2μg/10μl) and chloroform extract (2.8μg/10μl). The other phytochemicals like total phenol, flavonoids and total sugars were low in concentration in the dialyzed Coleus aromaticus protein extract compared to hydro alcoholic extract, ethanol extract and chloroform extract.

Conclusions: In this study, the protein concentration in dialyzed Coleus aromaticus protein extract is considerably higher than the same in other solvent extracts. This is the basis for further studies to unfold the antioxidant activity of Coleus aromaticus protein in vitro.


Wadikar DD, Patki PE. Coleus aromaticus: A therapeutic herb with multiple potentials. J Food Sci Technol. 2016;53(7):2895-901.

Soni H, Singhai AK. Recent updates on the genus Coleus: a review. Asian J Pharm Clin Res. 2012;5(1):12-7.

Candrappa SM, Hugar S, Itgappa M, Nagarajappa K. Antidiabetic and antioxidant potential of coleus aromaticus leaf extracts in alloxan induced diabetic rats. Pharmacologyonline. 2009;3:1054-64.

Chandrappa MS, Harsha R, Dinesha R, Gowda TS. Antibacterial activity of coleus aromaticus leaves. Inter J Pharm Pharma Sci. 2010;2:63-6.

Khare RS, Banerjee S, Kundu K. Coleus aromaticus Benth-A nutritive medicinal plant of potential therapeutic value. Inter J Pharma Bio Sci. 2011;2(3):488-500.

Rai VM, Pai VR, Kedilaya PH, Hegde S. Preliminary Phytochemical Screening of members of Lamiaceae family: Leucas linifolia, coleus aromaticus and Pogestemon patchouli. International J Pharma Sci Review Res. 2013;21(1):131-7.

Ramya BS, Ganesh P, Kumar RS. Phytochemical screening of Coleus aromaticus and Leucas aspera and their antibacterial activity against enteric pathogens. Inter J Pharma Bio Archives. 2012;3(1):162-6.

Bole S, Kumudini, J. Phytochemical screening and biological activities of medicinal plant Coleus aromaticus. WJPPS. 2014;3(6):974-86.

Sasidharan S, Chen Y, Saravanan D, Sundram KM, Latha LY. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African J Traditional, Complementary Alternative Medicines. 2011;8(1).

Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American J Enology Viticulture. 1965;16(3):144-58.

Chang C, Yang M, Wen H, Chern J. Estimation of Total Flavonoid content in Propolis by Two Complementary methods. J Food Drug Analysis. 2002;10:178-82.

Dubois M, Giles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for the determination of sugars and related substances. Anal Chem. 1956;28:350-6.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem. 1976;72:248-54.

Badami S, Channabasavaraj KP. In Vitro. Antioxidant Activity of Thirteen Medicinal Plants of India's Western Ghats. Pharmaceutical biology. 2007;45(5):392-6.

De Mejia EG, Dia VP. The role of nutraceutical proteins and peptides in apoptosis, angiogenesis, and metastasis of cancer cells. Cancer Metastasis Rev. 2010;29(3):511-28.




How to Cite

Kumar N., S., Nusrath, A., & Ramadas, D. (2018). Quantitative analysis of chemical constituents in medicinal plant coleus aromaticus extracts. International Journal of Research in Medical Sciences, 6(3), 1002–1005. https://doi.org/10.18203/2320-6012.ijrms20180630



Original Research Articles