Published: 2018-03-28

Determination of the dose and time dependent toxicological effects of hydroalcoholic extract of Terminalia catappa kernel on the renal functions parameters of wister rats

Nimisoere P. Batubo


Background: The use of herbal medications has been implicated to cause renal toxicity and failure. Terminalia catappa has been used in ancient tradition in the treatment several diseases. This study aimed to determine the dose and time related effects of the hydroalcoholic extract of Terminalia catappa on the renal functions of Wister rats following long time period of administration.

Methods: Forty female Wister rats were used as experimental animals and equally divided into 5 groups. The hydroalcoholic extract of Terminalia catappa were administered on the experimental animals for 42 days. Three groups were treated orally with three different doses of Terminalia catappa hydroalcoholic extract; 0.5g/kg, 1.0g/kg and 3.0g/kg respectively while another two groups as control. Signs of toxicity in the experimental rats were determined by the number of death, nutritional behaviour and physiological characteristics of the animal by observation while blood sample was collected for the determination of renal functions parameters.

Results: No death was recorded in all test groups during the period of the study. The nutritional behaviour of the rats was not affected and no abnormalities in the physiological characteristic of the rats were detected. The renal functions parameters (Na+, K+, Cl-, HCO3-, Urea, Uric acid and Creatinine) were not significantly (p>0.05) altered.

Conclusions: Based on the results, it can be concluded that there were no nephrotoxic effects of hydroalcoholic extract of Terminalia catappa on Wister rats and therefore it can be said to have a wide margin of safety for use and consumption.


Hydroalcoholic extract, Long-term treatment period, Renal functions parameters, Toxicological effects, Terminalia catappa kernel, Wister rats

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Wachukwu CM, Emem-Chioma PC, Wokoma FS, Oko-Jaja RI. Prevalence of risk factors for chronic kidney disease among adults in a university community in southern Nigeria. Pan Afr Med J. 2015;21:120.

Asif M. A brief study of toxic effects of some medicinal herbs on kidney. Advanced biomedical research. 2012;1.

Gao J, Tang X, Dou H, Fan Y, Zhao X, Xu Q. Hepatoprotective activity of Terminalia catappa L. leaves and its two triterpenoids. J Pharm Pharmacol. 2006;56:1449-55.

Mininel FJ, Leonardo Junior CS, Espanha LG, Resende FA, Varanda EA, Leite CQ, et al. Characterization and quantification of compounds in the hydroalcoholic extract of the leaves from Terminalia catappa Linn. (Combretaceae) and their mutagenic activity. Evidence Based Complement Alternate Med. 2014:676902.

Fan YM, Xu LZ, Gao J, Wang Y, Tang XH, Zhao XN, Zhang ZX, et al. Phytochemical and anti-inflammatory studies on Terminalia catappa. Fitoterapia. 2004;75:253-260.

Chen PS, Li JH. Chemopreventive effects of punicalagin, novel tannin components isolated from Terminalia catappa, on H-ras-transformed NIH3T3 cells. Toxicology Letters. 2006;163:44-53.

Nagappa AN, Thakurdesai PA, Venkat Rao N, Singh J. Antidiabetic activity of Terminalia catappa Linn fruits. J Ethnopharmacol. 2003;88:45-50.

Ahmed SM, Swamy BMV, Dhanapal PGR, Chandrashekara VM. Antidiabetic activity of Terminalia catappa Linn. leaf extracts in allox an induced diabetic rat. Ira J Pharmacol Therapeutics IJPT. 2005;4:36-39.

Pawar SP, Pal SC. Antimicrobial activity of extracts of Terminalia catappa root. Indian J Med Sci. 2002;56(6):276-8.

Goun E, Cunningham G, Chu D, Nguyen C, Miles D. Antibacterial and antifungal activity of Indonesian ethnomedical plants. Fitoterapia 76. 2003:237-243.

Tan R. Sea Almond Tree: Terminalia catappa, 2001. Available at sea_almond.htm. Accessed on 1 January 2017.

Donegan K, Matyac C, Seidler R, Porteous A. Evaluation of methods for sampling, recovery, and enumeration of bacteria applied to the phylloplane. Appl Environ Microbiol. 1991;57(1):51-6.

Rosenbaum PR. Sampling the leaves of a tree with equal probabilities. J Ame Statistical Association. 1993;88(424):1455-7.

Makkar HP. Quantification of tannins in tree foliage-a laboratory manual, a Joint FAO. IAEA working document, Vienna, Austria. 2000.

Makkar HPS. Chemical and biological assays for quantification of major plant secondary metabolites. In: Sandoval-Castro C.A, DebHovell FD, Torres-Acosta JFJ and Ayala-Burgos. (Eds.), Herbivores, assessment of intake, digestibility and the roles of secondary compounds. Nottingham University Press, Nottingham. 2006:235-249.

OECD. Organization for Economic Cooperation and Development, OECD test guideline for testing of chemicals 420. Acute oral toxicity-fixed dose procedure. 2001:1-14.

Levine BS. Animal clinical pathology. CRC handbook of toxicology. 1995;2:742-68.

Evans GO. General introduction. In: Animal Clinical Chemistry (Editor, G. O. Evans). Taylor & Francis, London, United Kingdom. 1996:1-10.