Correlation of serological markers and thrombocytopenia in early diagnosis of dengue infection
Keywords:Dengue fever, IgG antibody, IgM antibody, NS1 antigen
Background: Dengue is an acute viral infection with fatal complications. To reduce the disease burden, early and specific diagnosis of dengue hemorrhagic fever or dengue shock syndrome with supportive therapy reduces morbidity and mortality. This study was undertaken to evaluate the efficacy of NS1 antigen and platelet count.
Methods: A total of 200 serum samples were collected from clinically suspected cases of dengue fever. Serum samples were tested for NS1, IgM and IgG and correlated with the platelet count.
Results: Out of 200 samples screened in our study, 100 (50%) were positive for dengue parameters, 100 (50%) were negative. Out of 100 positive cases, 95 (95%) cases were positive for NS1antigen either alone or in combination with antibodies. 84 (84%) cases were exclusively positive for NS1 antigen only. Primary infection (positive for NS1 Ag, IgM, NS1 + IgM) was seen in 99 (99%) cases and secondary infection (positive for IgG, NS1 + IgG, IgM + IgG, NS1+ IgM+ IgG) was seen in 1 case (1%). Out of 100 positive cases, thrombocytopenia was observed in 60 (60%) of patients while 40 (40%) had platelet count within normal range.
Conclusions: Dengue is endemic to Indian subcontinent. Currently no specific antiviral therapy is available. Measuring platelet count and detecting the NS1 antigen plays a vital role in early diagnosis, management and implementing the control measures in community to avoid spread of dengue.
Yboa, Begonia C., and Leodoro J. Labrague. "Dengue Knowledge and Preventive Practices among Rural Residents in Samar Province, Philippines." American Journal of Public Health Research 1.2 (2013): 47-52.
Heymann DL. Control of communicable disease manual. Eighteenth ed. Washington, DC: American Public Association; 2004. pp. 146-52.
World Health Organization (2009) Dengue Guidelines for Diagnosis, Treatment, Prevention, and Control: New Edition. Genev, 2004.
Egger JR, Ooi EE, Kelly DW,Woolhouse ME, Daviesa CR, ColemanaPG. Reconstructing historical changes in the force of infection of dengue fever in Singapore: implications for surveillance and control. Bulletin of the World Health Organization 2008; 86:187-96.
W K Cheah, K S Ng, Abd-Rahman Marzilawati, A Review of Dengue Research in Malaysia, Med J Malaysia Vol 69 Supplement A August 2014
Tong SF, Aziz NA, Chin GL. Prevalence of non-dengue thrombocytopaenia among adult patients presenting with acute febrile illness in primary outpatient clinics. Medicine & health 2006; 1(1): 25-30.
Tricou V, Hang TV, Quynh NV, Nguyen CV, Tran HT, Farrar J, et al. Comparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses. BMC Infect Dis 2010;10:142.
Sindhanai V, Sageera Banoo , Rajkumar N , Suresh Chander, Evaluation of Correlation between Dengue Serological Markers and Platelet Count, Sch. J. App. Med. Sci., 2016; 4(2D):618-622
Pimpan P, Prasert T; Clinical laboratory investigations. Monograph on dengue/dengue haemorrhagic fever, WHO SEARO; 1993; 62- 71.
Pruthvi D, Shashikala P, Shenoy V; Evaluation of Platelet Count in Dengue Fever Along with Seasonal Variation of Dengue Infection. J Blood Disorders Transf 2012; 3:128. doi:10.4172/2155-9864.1000128
Kulkarni SK , Sch. J. App. Med. Sci., March 2016; 4(3A):649-652
Mohan DK, Shiddappa DM; A Study of Clinical Profile of Dengue Fever in a Tertiary Care Teaching Hospital Sch. J. App. Med. Sci., 2013; 1(4):280-282.
Shivraj Nagnath Kanthikar, Varsha Tukaram Kalshetti, Correlation of thrombocytopenia and serological markers in early diagnosis of dengue infection with special reference to NS1 antigen, Indian Journal of Pathology and Oncology, July-September 2016;3(3);437-439
Saroj Golia, Vivek Hittina halli, K. Sujatha Karjigi, Mallika Reddy, Asha S Kamath; Serodiagnosis of dengue using NS1 antigen, dengue IgM, dengue IgG antibody with correlation of platelet counts. International Journal of AJ Institute of Medical Sciences 2012; 1(2).
Mohd. Siddique Ahmed Khan, Mohd.Ibrahim Shaikh, Shaikh Mahmood, Mymoona.SK, PROFILE OF SEROLOGICAL, HAEMATOLOGICALAND BIOCHEMICAL DYNAMICS IN DENGUE FEVER, International Journal of Scientific Research, Volume-6 | Issue-11 | November-2017
Kotresh Doddamane, Jayalakshmi MK, A Study of Clinical and Laboratory Profile of Dengue Fever in a Tertiary Care Centre, Sch. J. App. Med. Sci., February 2016; 4(2C):504-508