DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20162786

A study to assess serum levels of superoxide dismutase and catalase in senile cataract patients with and without diabetes mellitus at tertiary care hospital

Mohanty Lipa, Behera Madhusmita, Purohit Ajay

Abstract


Background: The objective was to assess the possible relationship of serum levels of superoxide Dismutase and catalase and development of senile cataract in patients with and without diabetes mellitus.

Methods: The study was done in the Department of Ophthalmology with support of Department of Biochemistry at Geetanjali Medical College and Hospital, Geetanjali University, Udaipur, Rajasthan. The study has been conducted during the time period of October 2014 and October 2015. Blood samples of 120 senile cataract patients, i.e. 60 with diabetes and 60 without diabetes were taken and levels of superoxide dismutase and catalase were studied.

Results: Statistically significant difference in age wise occurrence of cataract was found between diabetic and non-diabetic patients i.e. cataract was found at a later age in senile non diabetic patients as compared with senile diabetic patients (p<0.001). Senile diabetic patients had significantly lower serum level of SOD as compared to senile non diabetic patients (p<0.001 for both). Similarly, senile diabetic patients had significantly lower serum levels of catalase as compared to senile non diabetic patients (p<0.001 for both).

Conclusions: Increase in oxidative stress and decrease in anti-oxidant enzyme activities have a role in the early development of cataract in senile patients with diabetes. Thus, diabetic cataract seems to be associated with decrease in serum levels of SOD and catalase.


Keywords


Serum level, Cataract, Diabetes mellitus, Oxidative stress, Superoxide dismutase, Catalase

Full Text:

PDF

References


Fernandez MM, Afshari NA. Nutrition and the prevention of cataracts. Curr Opin Ophthalmol. 2008;19:66-70.

Lou MF. Redox regulation in the lens. Prog Retin Eye Res. 2003;22:657-82.

Spector A. Oxidative stress-induced cataract: Mechanism of action. FASEB J. 1995;9:1173-82.

Maulik N, Das DK. Emerging potential of thioredoxin and thioredoxin interacting proteins in various disease conditions. Biochim Biophys Acta. 2008;1780(11):1368-82.

Srivastava SK, Ramana KV, Bhatnagar A. Role of aldose reductase and oxidative damage in diabetes and the consequent potential for therapeutic options. Endocr Rev. 2005;26:380-92.

Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma Malondialdehyde as biomarker for oxidative stress: Reference interval and effects of life-style factors. Clin Chem. 1997;43:1209-14.

Chung SS, Ho EC, Lam KS, Chung SK. Contribution of polyol pathway to diabetes-induced oxidative stress. J Am Soc Nephrol. 2003;14:233-6.

Ozmen B, Ozmen D, Erkin E, Güner I, Habif S, Bayindir O. Lens superoxide dismutase and catalase activities in diabetic cataract. Clin Biochem. 2002;35:69-72.

Ateş NA, Yildirim O, Tamer L, Unlü A, Ercan B, Muşlu N, et al. Plasma catalase activity and malondialdehyde level in patients with cataract. Eye. 2004;18:785-8.

Obara Y. The oxidative stress in the cataract formation. Nippon Ganka Gakkai Zasshi. 1995;99:1303-41.

Babizhayev MA. Failure to withstand oxidative stress induced by phospholipid hydroperoxides as a possible cause of the lens opacities in systemic diseases and ageing. Biochim Biophys Acta. 1996;1315:87-99.

Micelli-Ferrari T, Vendemiale G, Grattagliano I, Boscia F, Arnese L, Altomare E, et al. Role of lipid peroxidation in the pathogenesis of myopic and senile cataract. Br J Ophthalmol. 1996;80:840-3.