Published: 2018-06-25

A scientific validation of In vitro anti-inflammatory activity of Punica granatum L. by human red blood cell membrane stabilization

Karunakar Kota, Sandhya Sharma, Jameela Tahashildar


Background: In recent years there has been growing interest in therapeutic use of natural products, especially those derived from plants. P. granatum is very common dietary ingredient in many parts of India and has remarkable biological and medicinal properties.

Methods: In the present study, the methanolic extract of fruit peels of Punica granatum Linn. (MEPG) were investigated for anti-inflammatory activity by simple, reliable, less toxic and less time consuming HRBC membrane stabilization method. The presentation of hypo tonicity induced HRBC membrane lysis was taken a measure of anti-inflammatory activity. Their activities were compared with standard drug diclofenac.

Results: The results of the study demonstrated that P. granatum contains active constituents, which possess anti-inflammatory activity which is probably related to the inhibition of prostaglandin synthesis.

Conclusions: It is concluded that methanolic extract of P. granatum fruit peel possesses significant anti-inflammatory activity and this is a possible rationale for its folkloric use as an anti-inflammatory agent.


Anti-inflammatory, HRBC membrane stabilization, Methanolic extract, Punica granatum Linn

Full Text:



Vane JR and Botting RM. New insight in to the mode of action of anti-inflammatory drugs. Infla Res. 1995;44:1-10.

Rajendran V, Lakshmi KS. In vitro and in vivo anti-inflammatory activity of leaves of symplocosco chinchinensis (Lour) Moore. Bangladesh J Pharmacol. 2008;3:121-4.

Seema CC, Sharan SV, Rao BS, Meena V. In vitro anti-inflammatory activity of methanolic extract of centella asiatica by hrbc membrane stabilization. Inter J Chem, Environ Pharma Res. 2011;4(2):457-60.

Koleva II, Van Beek TA, Linssen JP, Groot AD, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical analysis. 2002 Jan 1;13(1):8-17.

Kota KR, Sharma S, Tahashildar J. Amelioration of oxidative stress by ethanolic extract of the fruit of lagenaria siceraria in rabbits consisting of carbon tetrachloride induced hepatotoxicity. World J Pharma Res. 2016;5(2):648-60.

Rastogi RP, Mehrotra BN. Compendium of Indian medicinal plants: A CDRI series. Lucknow, Publication and information Directorate, New Delhi, 4th vol. 1995;597.

Asolkar LV, Kakkar KK, Chakre OJ. Glossary of Indian medicinal plants with active principles (part 1). CSIR, New Delhi. 1992;117:1965-81.

Gamble JS. Flora of Presidency of Madras, 1921. Vol II, Bishen Singh Mahendra Pal Singh Dehra Dun. London.1921:589.

Ouachrif A et al. Comparative study of the anti-inflammatory and antinociceptive effects of two varieties of Punica granatum. Pharmaceutical Biology 2012;50(4):429-38.

Jafri MA, Aslam M, Javed K, Singh S. Effect of Punica granatum Linn. (flowers) on blood glucose level in normal and alloxaninduced diabetic rats. J Ethnopharmacol. 2000;70:309-14.

Das AK, Mandal SC, Banerjee SK, Sinha S, Das J, Saha BP, Pal M. Studies on antidiarrhoeal activity of Punica granatum seed extract in rats. J Ethnopharmacol. 1999;68:205-8.

Ajaikumar KB, Asheef M, Babu BH, Padikkala J. The inhibition of gastric mucosal injury by Punicagranatum L. (pomegranate) methanolic extract. J Ethnopharmacol. 2005;96:171-6.

Prakash G, Yoganandam, K.Ilango, Sucharita De. Evaluation of anti-inflammatory and membrane stabilizing properties of various extracts of Punica granatum L.(Lythraceae). International J Pharm Tech Res. 2010;2(2):1260-63.

Farouk L, Laroubi A, Aboufatima R, Benharref A, Chait A. Evaluation of the analgesic effect of alkaloid extract of Peganum harmala Linn: possible mechanisms involved. J Ethnopharmacol. 2008;115:449-54.

Gandhisan R, Thamaraichelvan AB. Anti-inflammatory action of Lannea coromandelica HRBC membrane stabilization. Fitotherapia. 1991;62:82-3.

Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol. 2007;147(2).

Abbas AK, Lichtman AH, Pillai S. Basic immunology: functions and disorders of the immune system. Elsevier Health Sciences; 3rd ed. 2009.

Seema CC, Sharan SV, Srinivasa RB and Meena V. In vitro anti inflammatory activity of Methanolic extract of centella asiatica by HRBC membrane stabilization. RJC. 2011;4(2):457-60.

Shirwaikar A, Devi S, Siju EN. Anti-Inflammatory activity of Thespesia populnea fruits by Membrane Stabilization. Inter J Pharm Tech Res. 2011 Oct;3(4):2060-3.

Chippada SC, Volluri SS, Bammidi SR and Vangalapati M. In vitro anti inflammatory activity of methanolic extract of centella asiatica by hrbc membrane stabilisation. Rasayan J Chem. 2011;4(2):457-60.

Okoli CO, Akah PA. Mechanism of the anti-inflammatory activity of the leaf extracts of Culcasia scandens P. beauv (Araceae). Pharmacol Biochem Beha. 2004;79:473-81.

Lavanya R, Maheshwari S, Harish G, Bharath J, Kamali S, Hemamalani D, et al. Investigation of In-vitro anti-Inflammatory, anti-platelet and anti-arthritic activities in the leaves of Anisomeles malabarica Linn. Res. J Phar Biol Chem Sci. 2010;1:745-52.