Lipid peroxidation and superoxide dismutase activities in patients with type 2 diabetes complicated with peripheral neuropathy
DOI:
https://doi.org/10.18203/2320-6012.ijrms20160295Keywords:
Oxidative stress, Diabetic neuropathy, Antioxidants, TBARSAbstract
Background: Diabetic neuropathy is one of most common complication of diabetes. The suggested factor for the development of diabetic neuropathy is increased lipid peroxidation which is a result of enhanced free radical generation and decreased antioxidant defence.
Methods: This cross sectional study was carried out in the department of Biochemistry, JSS Medical College, Mysore. Thirty patients with diabetic neuropathy, 30 diabetics without any complications & 30 age and sex matched controls were included in the study. Glycated haemoglobin, superoxide dismutase were estimated in whole blood by latex agglutination inhibition assay and INT reduction method respectively. Serum thiobarbituric acid reactive substance (TBARS) was estimated by thiobarbituric acid assay. SPSS version 16 for windows was employed for statistical analysis.
Results: Mean serum TBARS levels were significantly greater in diabetic neuropathy. There was significant negative correlation between serum TBARS and SOD in cases (r=-0.176). Highly significant negative correlation was found between SOD and HbA1c in cases (r=-0.168).
Conclusions: There exists an inverse relationship between TBARS and antioxidants in diabetic neuropathy and is a result of poor glycaemic control.
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References
Boulton AJM, Vinik AI, Arezzo JC, Feldman EI, Freeman R, Malik RA. Diabetic neuropathies. Diabetes Care. 2005;28:956-62.
Singh N, Armstrong DG, Lipsky BA. Preventing foot ulcers in patients with diabetes. JAMA. 2005;293:217-228.
Feldman EL, Russell JW, Sullivan KA, Golovoy D. New insights into the pathogenesis of diabetic neuropathy. Curr Opin Neurol. 1999;5:553-63.
Jakus V. The role of free radicals, oxidative stress and antioxidant system in diabetic vascular diseases. Bratesl Lek Listy. 2000;101(10):541-51.
Bannister WH, Bannister JV. Biological and clinical aspects of superoxide and superoxide dismutase. In: The Biology and Chemistry of active oxygen. New York: Elsevier. 1984:164.
Bunn HF. Non enzymatic glycosylation of protein: relevance to diabetes. Am J Med; 1981;70:325-30.
Sacks DB. Carbohydrates. 4th ed. In. Teitz Textbook of Clinical Biochemistry, Burtis CA, Ashwood ER, Burns DE, eds. Philadelphia: WB Saunders. 2006:837-901.
Kurnet D, Tappe KJ. Effect of vitamin E, Ascorbic acid and mannitol on alloxan induced lipid peroxidation in rats. Arch. Biochemistry and Biophysics. 1982;216(1):204-12.
Erum SK. Lipid peroxide in cerebrovascular disorder determined by a new colorimetric method. Clin Chem Acta. 1978;90:37-43.
Bansal V, Kalita J, Misra UK. Diabetic neuropathy. Postgrad Med J. 2006;82:95-100.
Pasupathi P, Chandrasekar V, Kumar US. Evaluation of OS, antioxidant and thyroid hormone status in patients with diabetes mellitus. J Medicine. 2009;10:60- 6.
Ziegler D, Sohr GC, Nourooz-Zadeh J. Oxidative Stress and Antioxidant Defence in Relation to the Severity of Diabetic Polyneuropathy and Cardiovascular Autonomic Neuropathy. Diabetes Care. 2004;27(9):2178-83.
Abou-Seif MA, Youssef AA Evaluation of some biochemical changes in diabetic patients. Clin Chim Acta. 2004;346(2):161-70.
Srivatsan R, Das S, Gadde R, Kumar KM, Taduri S, Rao N, et al. Antioxidants and Lipid Peroxidation Status in Diabetic Patients with and without Complications. Arch Iranian Med. 2009;12(2):121-27.
Kesavulu MM, Rao R, Giri R, Vijaya J, Subramanyam G, Apparao C. Lipid peroxidation and antioxidant enzyme status in type 2 diabetics with coronary heart disease. Diab Res Clin Pract. 2001;53:33-9.
Ramakrishna V, Jailkhani R. Evaluation of OS in Insulin Dependent Diabetes Mellitus (IDDM) patients. Diagn Pathol. 2007;2:22.
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