DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20183273

Matrix metalloproteinase-9 (MMP-9) as prospective histopathological and molecular biomarker for oral squamous cell carcinoma

Bhagyalakshmi Atla, Sreevalli Prabhakula, Seeram Satish Kumar, Lakshmi Saraswathi Boni

Abstract


Background: Despite the advance of therapeutic approaches, the morbidity and mortality of oral squamous cell carcinoma have not improved significantly. The invasion potential of oral squamous cell carcinoma is conferred by their ability to utilize Matrix metalloproteinase that are produced by the host’s stromal cells. In the present study an effort was made to analyse the role of clinical features and risk factors in the causation of oral squamous cell carcinoma and expression of Matrix metalloproteinase-9 (MMP-9) was studied in various grades of squamous cell carcinoma with its role in metastasis.

Methods: The present study is an hospital based prospective study for a period of two years at the department of pathology from August 2014 to July 2016 consisting of 88 cases of oral squamous cell carcinoma. Tissue for H and E sections were fixed in 10% formalin and subjected to routine paraffin embedded processing and stained with hematoxylin and eosin.

Results: Total number of cases of oral squamous cell carcinoma analyzed were 88, out of which 65 cases were subjected to MMP 9 expression by random selection on Immunohistochemistry. Majority of cases occurred in the age group of 41-50 years with male: female ratio of 1.4:1. In stage I and II the expression of MMP-9 is low in 37 cases (82.22%) and in stage III the expression is high in 14 cases (70%) with P value <0.05 (significant).

Conclusions: Matrix metalloproteinases could act as a therapeutic target and could play an important role in effective treatment strategies of oral cancer.


Keywords


Matrix metalloproteinase-9, Oral cancer, Squamous cell carcinoma

Full Text:

PDF

References


Massano J, Regateiro FS, Januario G, Ferreira A. Oral squamous cell carcinoma: review of prognostic and predictive factors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102:67-76.

Mishev G, Deliverska E, Hlushchuk R, Velinov N, Aebersold D, Weinstein F, et al. Prognostic value of matrix metalloproteinases in oral squamous cell carcinoma. Biotechnol biotechnological equipment. 2014 Nov 2;28(6):1138-49.

Aebersold DM, Beer KT, Laissue J, Djonov V, Greiner RH. Intratumoral microvessel density predicts local treatment failure of radically irradiated squamous cell cancer of the oropharynx. Int J Radiat Oncol Biol Phys. 2000;48:17-25.

Jayade BV, Bhat K, Patil BR, Nayak R, Sant A. Histological significance of p53 gene expression in squamous cell carcinoma of the buccal mucosa. J maxillofacial oral surgery. 2009 Sep 1;8(3):205.

Vaidhehi Narayan Nayak et al. Oral squamous cell carcinoma: A 5-years institutional study. J Med Radiol Pathol Surg. 2015;1:3-6.

Ayaz B, Saleem KH, Azim WA, Shaikh AL. A clinico-pathological study of oral cancers. Biomedica. 2011 Jan;27(1):29-32.

Zulfiqar A, Nagi AH, Nasim NA. A clinicopathological study of orofacial squamous cell carcinoma in local population. Biomedica. 2013 Jul;29(3):147-50.

Pires FR, Ramos AB, Oliveira JB, Tavares AS, Luz PS, Santos TC. Oral squamous cell carcinoma: clinicopathological features from 346 cases from a single oral pathology service during an 8-year period. J App O Sci. 2013 Oct;21(5):460-7.

Kuriakose M, Sankaranarayanan M, Nair MK, Cherian T, Sugar AW, Scully C, et al. Comparison of oral squamous cell carcinoma in younger and older patients in India. Eur J Cancer B Oral Oncol. 1992;28B:113-20.

Manuel S, Raghavan SK, Pandey M, Sebastian P. Survival in patients under 45 years with squamous cell carcinoma of the oral tongue. Int J Oral Maxillo fac Surg. 2003;32:167-73.

Monteiro-Amado F, Castro-Silva II, Lima CJ, Soares FA, Kowalski LP, Granjeiro JM. Immunohistochemical evaluation of MMP-2, MMP-9 and CD31/microvascular density in squamous cell carcinomas of the floor of the mouth. Brazilian dental J. 2013;24(1):3-9.

Ravi DK, Kumar M, Singh G, Rai SB, Chincholkar T, Saxena AK, et al. Matrix Metallopeptidase 9 (MMP-9) Expression in Oral Cancer Andand Adjacent Normal Tissue. World J Surg Med Radia Oncol. 2014 Dec 15;3(17).

Fan HX, Li HX, Chen D, Gao ZX, Zheng JH. Changes in the expression of MMP2, MMP9, and ColIV in stromal cells in oral squamous tongue cell carcinoma: relationships and prognostic implications. J Exp Clin Can Res. 2012;31(1):90.

Andisheh-Tadbir A, Mardani M, Pourshahidi S, Nezarati K, Bahadori P. Prognostic value of matrix metalloproteinase-9 expression in oral squamous cell carcinoma and its association with angiogenesis. J Clin Exp Den. 2016 Apr;8(2):e130.