Serological and molecular approaches for leptospirosis at a tertiary care centre in northern India

Saurabh Chaurasia; Raj Kumar Kalyan; Prashant Gupta; Kamlesh K. Gupta; Chandra Kanta; Akanksha Gupta

doi:10.18203/2320-6012.ijrms20183649

Authors

Saurabh Chaurasia Department of Microbiology, Era’s Lucknow Medical University, Lucknow, Uttar Pradesh, India
Raj Kumar Kalyan Department of Microbiology, King George’s Medical University, Lucknow, Uttar Pradesh, India
Prashant Gupta Department of Microbiology, King George’s Medical University, Lucknow, Uttar Pradesh, India
Kamlesh K. Gupta Department of Medicine, King George’s Medical University, Lucknow, Uttar Pradesh, India
Chandra Kanta Department of Paediatric, King George’s Medical University, Lucknow, Uttar Pradesh, India
Akanksha Gupta Department of Microbiology, King George’s Medical University, Lucknow, Uttar Pradesh, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20183649

Keywords:

ELISA, Leptospirosis, Microscopic Agglutination Test, PCR, Pyrexia of unknown origin

Abstract

Background: Aims and objectives of the study was to determine prevalence rate of leptospirosis and recognition of common epidemiological situation and clinical manifestations of leptospirosis in patients with pyrexia of unknown origin at a tertiary care centre.

Methods: This was a hospital based prospective observational study. The duration of study was from August 2015 to July 2016. Patients with clinical symptoms of pyrexia of unknown origin attending Gandhi Memorial and Associated Hospital, King George’s Medical University Lucknow during the study period were enrolled in this study. We performed the IgM ELISA and PCR for the leptospirosis at our centre and for the Micro Agglutination Test (MAT), we sent the serum samples to regional medical research centre Department of Health Research Ministry of Health and Family Welfare, Govt. of India Port Blair.

Results: A Total of 104 symptomatic patients were recruited. Of total, IgM ELISA for leptospirosis were positive in 25 patients, PCR in 20 patients and MAT shows significant titres in 3 samples. The ROC curve analysis revealed significant diagnostic accuracy of IgM ELISA with 100.00% sensitivity and 78.22% specificity however showed less positive predictive value (12.0%) but high negative predictive value (100.0%). Most common presentation were fever, jaundice and rashes (40.4%) followed by fever and jaundice (29.8%).

Conclusions: Leptospirosis IgM ELISA and PCR showed good detection accuracy. Age, sex, occupation is not significantly associated to the leptospirosis.

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