DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20184431

High intake of green tea decreased hemoglobin and hematocrit levels in Rattus novergicus strain wistar albino

Sestia Rani, Rosfita Rasyid, Desmawati Desmawati

Abstract


Background: Anemia is one of four nutritional problems in Indonesia with a prevalence of 21.7%, the type of anemia is iron deficiency anemia. One of the causes of iron by tannins present in green tea. The purpose of this study is to determine the effect of green tea on hemoglobin and hematocrit amount in Rattus novergicus strain wistar albino.

Methods: This research used experiment with post-test only control group design. Twenty four rats were divided into four groups: the control group and the treatment group were given a different dose of 5.6g in a 1.8ml/200gr solvent, 8.4g in a 1.8ml/200g solvent, 11.2g in 1.8ml/200gr solvent for a month. Blood is taken through 2 ml orbital sinus using capillary pipette. hemoglobin examination using spectrophotometer and hematocrit checking using microhematocrit tool. The study was conducted in pharmaceutical laboratories and biochemical laboratories. Data analysis using one way ANOVA and Kruskal Wallis with degree of significance p<0.05.

Results: The results of the study were found the mean differences amount of hemoglobin and hematocrit. The mean of control group in hemoglobin content was 16.39gr%, in treatment group were P1: 13.64gr%, P2: 11.19gr% and P3: 10.49 gr%, while in hematocrit control group was 44.83%, in treatment group were P1: 44.83%, P2: 39.17% and P3: 37.17%. Result of the test using one way ANOVA, which found the effect of green tea on hemoglobin (p value = 0.000) and result of the test using Kruskal-Wallis, which found the effect of green tea on hematocrit (p value = 0.017).

Conclusions: Based on the results of this study concluded that there is influence of green tea on the decrease of hemoglobin and hematocrit amount in Rattus novergicus strain wistar albino. It is suggested to pay more attention to dose of green tea with normal limit of 8.4gr.


Keywords


Green Tea, Hematocrit, Rat (Rattus novergicus), Level of hemoglobin

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References


Winarno W, Kristiano K. Green tea and white Tea. Jakarta. PT. Gramedia Main Library. 2016:44-104.

Delimont NM, Haub MD, Lindshield BL. The impact of tannin consumption on iron bioavailability and status: a narrative review1. Current Develop Nutr. 2017 Jan 19;1(2):1-2.

Scollrary GR. GWRDC Tannin review. Grape and Wine Research and Development Corporation. 2010. Available at https://www.wineaustralia.com/getmedia/b1726202-39fb-4312-aa50-5703c67c668d/GWR-0905.

Setiyarno S, Anggraeni T, Mustaan. Relationship between tea consumption and hemoglobin amount in the district of Jenawi in Karanganyar Regency. Nursing Science. J Public Health 2012;1(1):1-13.

Guyton A, dan Hall J. Medical Physiology Textbooks, 11th ed. Jakarta: EGC. 2014: 445-3.

Indonesia Ministry of Health. Indonesian health profile, 2013. Available at: http://www.depkes.go.id/resources/download/pusdat

in/profil-kesehatan-indonesia/profil-kesehatanindonesia-2013.pdf. Accessed 5 April 2017.

Indonesian Ministry of Health. Basic Health Research. 2013 Available at http://www.depkes.go.id/resources/download/general/Hasil%20Riskesdas%20/2013. Accessed 5 April 2017.

Padang City Health Office. Padang's health profile in 2012. 2013 edition Available at http://www.depkes.go.id/resources/download/profil/Profil_Kes_Provinsi_2012/03_Profil Kes_Prov.SumateraBarat_2012.pdf. Accessed 5 April 2017.

Padang City Health Office. Health profile of west sumatra province. 2012. 2013 edition Available at http://ww.depkes.go.id/resources/dowload/profil/profil kab kota /2015/1371 sumbar kota padang 2015.pdf. Accessed 5 April 2017.

Arisman MB. Nutrition in the life cycle. Jakarta: Publisher of the medicine book. 2010:16-118.

Bakta IM. Concise Clinical Hematology. Jakarta: Publisher of the Medicine Book. 2010: p 26-38.

Manach C, Williamson G, Morand C, Scalbert A, Rémésy C. Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am J Clin Nutr. 2005 Jan 1;81(1):230S-42S.

Balarajan Y, Ramakrishnan U, Özaltin E, Shankar AH, Subramanian SV. Anaemia in low-income and middle-income countries. Lancet. 2011 Dec 17;378(9809):2123-35.

Lubis TM, Zuhrawati, Susanti F, Rusli, Asmilia N, Muttaqien. The Influence of Green Tea Extract (Camelia Sinesis) toward decreasing hemoglobin amount and hematocrit value on mice wistar (Ratus Norvegicus. J Medika Veterinar. 2016;10(2):141-3.

Bait Y. The effectiveness of giving green tea (CameliaSinencis Var Assamica), mulbei leaf tea (MorusKanva) and the mix In Hypoglycemic Activity on Mice (Ratus novergicus). Bogor Agricultural Institution. 2010.

Hoffbrand AV, Pettit JE, Moss PAH. Capita Hematological Selections. 4th ed. Jakarta: Publisher of the Medicine Book;2005.

Hoong YB, Paridah MT, Luqman CA, Koh MP, Loh YF. Fortification of sulfited tannin from the bark of Acacia mangium with phenol–formaldehyde for use as plywood adhesive. Ind Crops Products. 2009 Nov 1;30(3):416-21.

Khaidir M. Iron deficiency anemia. J Public Health 2007;2(1):140-4.

Ismarani. Potential of tannin compounds in supporting eco-friendly production. J Agribusiness Regional Develop. 2012 3(2):46-55.

Besral B, Meilianingsih L, Sahar J. The effect of drinking tea to the anemia among elderly in Bandung. Makara J Health Res. 2010 Oct 14:38-43.

Suryaningrum R. Sulthan M, Profiadi S, Maghfiroh K. Efforts to increase value of tea bags. Malang. Department of Biology Education. FKIP. Muhammadiyah University, Malang. 2006. Available at: student-research.umm.ac.id/index.php/pkmi/article/download/26/27.

Ramlah, Determination of temperature and optimum time for brewing green tea leaves (Camellia Synthesis l.) against the content of antioxidant and caffeine, tannins, and catechins. State Islamic University, Faculty of Science and Technology. Makassar, 2017. Available at: http://repositori.uinalauddin.ac.id/4115/1/RAMLAH.pdf.