DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20184896

Effect of reduced glutathione on follicle stimulating hormone levels and spermatozoon quality of adult rat exposed to monosodium glutamate administration

Ayla Kartika, Fadil Oenzil, Eliza Anas

Abstract


Background: Excessive consumption of Monosodium Glutamate (MSG) causes oxidative stress of hypothalamic cells and testicular cells thus affecting the process of spermatogenesis. Reduced Glutathione (GSH), as an antioxidant can reduce oxidative stress. This study aimed to see the effect of GSH on Follicle Stimulathing Hormone (FSH) levels and the quality of spermatozoon in male rats given MSG.

Methods: This research used experiment with post test only control group design. The samples used were 27 male rats divided into 3 groups. The negative control group (KN) was given a standard diet, the positive control group (KP) was given MSG 144mg/200grbw, the treatment group (P) was given a standard diet and MSG dose was 144mg/200grbw and continued with GSH dose 25.2mg/200grbw. On the 52nd day, FSH levels were analyzed using ELISA, and the number, morphology and motility of spermatozoon were examined using a digital microscope.

Results: The results showed an average FSH level in the KN group (105.42ng/ml SD±64.44), KP (58.88 ng/ml SD± 22.1), P (64.57ng/ml SD±35.8) with a value of p = 0.072. The average number of spermatozoon in the KN group (43.59 million/ml SD ±14.06), KP (26.37 million/ml SD±12.7), P (43.26 million/ml SD±76.15) with a value of p = 0.007. The average normal morphology of spermatozoon in KN group (21.63% SD±10.9), KP (4.65% SD±4.38), P (23.18% SD±12.59) with p = 0.001. The average motility of spermatozoon in the KN group (57.58 million/ml SD± 11.24), KP (34.29 million/ml SD±21.60), P (72.33 million/ml SD±4.072) with a value p = 0.000.

Conclusions: Results of this study concluded that the administration of GSH did not significantly increase FSH levels. There is an effect of GSH on improving spermatozoon quality of rat given MSG.


Keywords


Follicle stimulating hormone levels, Monosodium glutamate, Reduced glutathione, Spermatozoon quality

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References


Ismail NH. Assessment of DNA damage in testes from young Wistar male rat treated with monosodium glutamate. Life Sci J. 2012;9:930-9.

Indonesian Ministry of Health. Riskesdas, Health research and development agency, 2013. Available at: www.depkes.go.id>download>general. Accessed February 2, 2017.

Septadina IS. Effect of monosodium glutamate on the reproductive system. Palembang: Sriwijaya University. 2017. Available at: http://eprints.unsri.ac.id/4040/1/makalahfungsional.pdf. 2014 Accessed February 2, 2017.

Hidayah RR, Amarwati S, Istiadi H. The effect of honey in liver microscopics appearance on wistar rats induced by monosodium glutamat. Media Medika Muda. 2015;4(4):1433-44.

Champe PC, Harvey RA, Ferrier RD. Biochemical Pictorial Reviews. 3th ed. Jakarta: EGC;2010.

Winarsi H. Natural antioxidants and free radicals. Yogyakarta, Kanisius. 2007:101-10.

Savira M. Impaired testicular development ADN decreased testosterone levels in experimental animal due to excessive exposure to monosodium glutamate. Researchgate. 2008.

Edward Z. The effect of giving monosodium glutamate to male rats (Rattus norvegicus) to levels of FSH and LH. Majalah Kedokteran Andalas. 2010:34(2).

Heffner LJ, Schust DJ. Reproduction System. 2nd ed. Jakarta, EGC;2008.

Fithriyah R, Tjandrakirana, Ducha N. The effect of green bean filtrate on the number of sertoli cells in the testes of mice exposed to monosodium glutamate. LenteraBio 2014:3(3).

Laksmi DNDI. Glutathion increases the quality of tubule seminiferous on mice having physical overtraining. Buletin Veteriner Udayana. 2010;2(1):11-9.

Sukanto H. Administration of glutathione prevents a decrease in total testosterone levels in mice that receive exposure to cigarette smoke, 2012. Available at: https://pps.unud.ac.id/.