Correlation between saturated fatty acid intake and superoxide dismutase activities with telomere length in Minangkabau ethnic men

Prima Minerva, Delmi Sulastri, Yuniar Lestari


Background: Non-communicable diseases (NCD) are the leading cause of death globally today. In West Sumatra there has been an increase in NCD which exceeds the national average percentage. Telomere shortening is associated with occurrence of NCD. Shortening of telomeres is influenced by diet. The purpose of this study was to examine the relationship between Saturated Fatty Acid (SAFA) intake and Superoxide Dismutase (SOD) activity with the telomere length of Minangkabau ethnic men.

Methods: This study was a cross sectional study. The sample of this study were 69 civil servants of Minangkabau male at district office in the Padang City, aged 40-50 years and according to the inclusion and exclusion criteria. Data on SAFA intake was obtained using Semi Quantitative Food Frequency Questionnaire (SQFFQ), SOD activity was measured using the Calorimetric method and telomere length was measured using Real Time Polymerase Chain Reaction (RT-PCR) by O’Challaghan and Fennech method. Data analysis was performed using Pearson correlation test with significance level p <0.05.

Results: The results showed an average SAFA intake 10.47+3.46% calories, SOD activity 5.93+0.81units/ml and telomere length 468.94+199.25bp. Correlation test between telomere length with SAFA intake showed (p=0.338), and the correlation test between SOD activity and telomere length showed (p=0.01).

Conclusions: From this study it was concluded that there was no correlation between SAFA intake and telomere length and there was a significant positive correlation between SOD activity and the telomere length of Minangkabau ethnic men.


Minangkabau ethnic men, Telomere, SAFA, SOD

Full Text:



Indonesia Ministry of Health. Overview of Non-Communicable Diseases in Indonesia 2009-2010: Center for Data and Information, 2012. Available at: Accessed March 3 2017.

Indonesia Ministry of Health. RISKESDAS: Basic Health Research, 2013. Available at: Accessed March 2, 2017

Nettleton JA, Roux AD, Jenny NS, Fitzpatrick AL, David RJ Jr. Dietary patterns, food groups and telomere length in the multi-ethnic study of atherosclerosis (MESA). Am J Clin Nutr. 2008;88(5):1405-12.

Grahame TJ, Schlesinger RB. Oxidative stress-induced telomeric erosion as a mechanism underlying air bone particulate matter-related cardiovascular disease. Particle and Fiber Toxicology. 2012;9(21):1-19.

J-Y, Jun NR, Yoon D, Shin C, Baik I. Association Between Dietary Patterns in The Remote Past and Telomere Length. Euro J Clin Nutri. 2015;69:1048-52.

QunXu, Parks CG, DeRoo LA, Cawthon RM, Sandler DP, Chen H. Multivitamin use and telomere length in women. Am J Clin Nutr. 2009;89:1-7.

Grahame TJ, Schlesinger RB. Oxidative stress-induced telomeric erosion as a mechanism underlying air bone particulate matter-related cardiovascular disease. Particle and Fiber Toxicol. 2012;9(21):1-19.

Boccardi V, Esposito A, Rizzo MR, Marfella R, Barbieri M, Paolisso G. Mediterranean diet, telomere maintenance and health status among elderly. PloS one. 2013 Apr 30;8(4):e62781.

Zhou M, Zhu L, Cui X, Feng L, Zhao X, He S, et al. Influence of diet on leukocyte telomere length, makers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study. Nutrition J. 2016;15(39):1-10.

Song Y, You NC, Song Y, Kang MK, Hou L, Wallace R, et al. Intake of small-to-medium-chain saturated fatty acids is associated with peripheral leukocyte telomere length in postmenopausal women-3. J Nutri. 2013 Apr 24;143(6):907-14.

Tiainen A-MK, Mannisto S, Blomstedt PA, Moltchanova E, Perata M-M, Kaartinen NE, et al. Leukocyte telomere length and its relation to food and nutrient intake in elderly population. Euro J Clin Nutri. 2012;66:1290-9.

Hatma RD, Lukito W, Rumawas YSP. Fatty acids intake among diverse ethnic group in Indonesia. Med J Indonesia. 2011;14(4):242-7.

Eszy M, Sastri S, Masri M. Effect of Giving High Palm Oil Diets on Malondialdehyde Levels in Wistar Rat Blood. Andalas Heal J. 2014;3(3):409-14.

Sayuti K, Yenrina R. Understanding antioxidants and their benefits. In: Antioxidants, Natural and Synthetic. Padang: Andalas University Press; 2015:7-14.

Michael JVR, Hekimi S. Superoxide dismutase is dispensable for normal animal lifespan. PNAS. 2012;109(15):5785-90.

Muchtadi D. Body's defense system against free radicals In: Nutrition for Anti-Aging. Bandung: Alfabeta Publisher; 2009:133-158.

Wang Y, Yang Y, Ma L, Zhao Y, Bai Z, Ge RL. Telomeres are elongated in rats exposed to moderate altitude. J Physiological Anthropol. 2014 Dec;33(1):19.

Hayes KC. Dietary fat and heart health: in search of the ideal fat. Asia Pacific J Clin Nutri. 2002;11:S394-S400.

Nojima M, Sakauchi F, Mori M, Tamakoshi A, Ito Y, Watanabe Y, et al. Relationship of serum superoxide dismutase activity and lifestyle in healthy Japanese adults. Asian Pacific J Cancer Prevention. 2009;10:37-40.

Kark JD, Goldberger N, Kimura M, Sinnreich R, Aviv A. Energy intake and leukocyte telomere length in young adults. Ame J Clin Nutri. 2012 Jan 11;95(2):479-87.

Fukai T, Folz RJ, Landmesser Ulf, Harrison DG. Extracellular superoxide dismutase and cardiovascular disease. Cardiovas Res. 2011;55:239-49.

Shin Y, Lee JH, Song W, Jun TW. Exercise training improves the antioxidant enzyme activity with no changes of telomere length. Research Institute of Sport Science, Seoul University Korea. 2008;129:254-60.

Chen J, Wei Y, Chen X, Jiao J, Zhang Y. Polyunsaturated fatty acids ameliorate aging via redox-telomere-antioncogene axis. Oncotarget. 2017;8(5):7301-14.