DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20190343

Histomorphometry of human vermiform appendix

Meenakshi Aggarwal, Hitant Vohra, Poonam Singh

Abstract


Background: Acute Appendicitis is one of the most common acute abdominal conditions. Advances in modern radiographic imaging have improved diagnostic accuracy, however the diagnosis of appendicitis remains essentially an enigmatic challenege. Though much work has been done on morphometry but there is less work done on variation of histomorphometric features of appendix. Hence the study was carried out by keeping the following objectives in mind. Aim was to study the general histomorphology and measure the histomorphometric parameters of human vermiform appendix. And also, to study diagnostic significance of histomorphology and histomorphometric parameters in causing appendicitis.

Methods: The study was conducted in the Department of Anatomy. Specimens of appendix were obtained from embalmed cadavers. Serial sections were taken at different levels and slides were prepared. The slides were then examined using Nikon Trinocular Research microscope under magnification power of 40x for various parameters, after staining with H&E.

Results: The mean luminal diameter varied from 1.32±0.65mm at base to 1.22±0.72mm from base. Diffuse lymphatic tissue was seen in both mucosa and sub-mucosa.

Conclusions: Definitely there is a relationship between lymphoid follicle diameter and mucosal-serosal thickness on one hand and that between the luminal diameter and lymphoid follicle diameter on the other hand. Since our study was restricted to geriatric age group so studies need to be done in different age groups to highlight any further relationship.


Keywords


Histomorphology, Histomorphometric parameters, Luminal Diameter, Vermiform appendix

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References


Dayal Y, Delellis RA. The Gastrointestinal Tract, In: Cot ran RS, Kumar V, Robbins SL, editors. Robbins Pathologic Basis of Diseases. 4th ed. Philadelphia: W.B. Saunders Company; 1989:827-910.

Day DW. Inflammatory disorders of the appendix. In: Day DW, Jess JR, Price AB, Shepherd NA, Sloan JM, Talbot IC, et al, editors. Morison and Dawson’s Gastrointestinal Pathology. 4th ed. Hong Kong. Blackwell Science; 2003:412-9.

Herrington JL Jr. The vermiform appendix: its surgical history. Contemp Surg. 1991;39:36-44.

Malla BK. A study on ‘vermiform appendix’-a caecal appendage in common laboratory mammals. Kathmandu University Med J. 2003;4:272-5.

Williams PL, Warwick R, Dyson M, Bannister LH, Moore REM, Holstead J, et al. Gray’s Anatomy. 36th ed. Elsevier Churchill Livingstone; 1980:1353-4.

Dhillon AP, Williams RA, Rode J. Age, site and distribution of sub epithelial neurosecretory cells in the appendix. Pathol. 1992;24:56-9.

DU Plesis DJ. The Gut. In: Lee McGregor’s Synopsis of Surgical Anatomy. 11th ed. Bombay: K.M. Varghese; 1975:55-67.

Foster CL. Digestive System-The Small and Large Intestine. In: Hewers’ Text Book of Histology for Medical Students. 7th ed. London: William Heine Medical Books Ltd; 1957:259-72.

Glover JW. The human vermiform appendix A general surgeon’s reflections, 1998. Available at: https://answersingenesis.org/human-body/vestigial-organs/the-human-vermiform-appendix/. Accessed on 15 November 2007.

Berry RJA. The vermiform appendix of Man, and the structural changes therein coincident with age. J Anat Physiol. 1906;40:247-56.

Rahman MM, Begum J, Khalil M, Latif SA, Nesa A, Jahan MK, et al, editors. Histomorphological study of lymphoid follicle of vermiform appendix. Mymen Singh Med J. 2008;17(2):134-40.

Shugaba AI, Umar MBT, Singh SP. Histomorphometric Profile of the human vermiform appendix. J Med Sci. 2006;6(3):445-51.

Sinonovsky V. Normal Appendix: Is there any significant difference in the maximal mural thickness at US between paediatric and adult population? Radiol. 2002;224:337-7.

Ali Malas M, Sulak O, Gokcimen A, Sari A. Development of the vermiform appendix during the foetal period. Surg Radiol Anat. 2004;26:202-7.