Effect of vitamin C on testosterone level, sperm count and sperm morphology in gentamicin-induced Wistar rats


  • Iis Rahayu Master Program, Graduate School of Biomedical Sciences, Faculty of Medicine, Andalas University, Padang, West Sumatera, Indonesia
  • Elly Usman Department of Pharmacology, Faculty of Medicine, Andalas University, Padang, West Sumatera, Indonesia
  • Mohamad Reza Department of Biology, Faculty of Medicine, Andalas University, Padang, West Sumatera, Indonesia




Gentamicin, Spermatozoa, Testosteron, Vitamin C


Background: Gentamicin is an aminoglycoside-class antibiotic that can lead to an increase in ROS and decrease antioxidant reserves that cause destruction of testicular cells that are known to inhibit cell division in testicular germ cells and protein synthesis in the testes as well as affect the production of testosterone that affects the quality of spermatogenesis. Vitamin C as an antioxidant can counteract ROS so that testicular cells can be repaired.

Methods: This study was an experimental research with post tests only control group design on 25 male rats, 2-3 aged months, weight 160-240 g. Animals were divided into 5 groups. Negative control group was placed in cage without treatment, positive control given induced gentamicin 5mg/kgbb for the first 10 days, and 3 treatment given induced gentamicin 5mg/kgbb for the first 10 days and at days 11-51 were given vitamin C at a dose of 1.6mg; 2.25mg; 4.5mg. On 52 day, all of wistar rats perform terminated for analyzed the testosterone levels were by ELISA, sperm count was calculated with then haemocytometer observed with digital microscope, morphology of spermatozoa was analyzed by eosin staining and was observed with digital microscope.

Results: Results of this study showed that the average of testosteron serum on control negatif group of 3,82ng/ml, control positif 3.73ng/ml, treatment 1 is 9.52ng/ml, treatment 2 is 8.29ng/ml, and traetment 3 is 2.28ng/ml. Sperm count on control negatif 41.68 million/ml, control positif 21.06million/ml, treatment 1 is 52.92 million/ml, treatment 2 is 57.12million/ml, treatment 3 is 80.88 million/ml and sperm morphology on control negative  74.00%, control positif 46.96%, treatment 1 is 58.82%, treatment 2 is 68.68%, and treatment 3 is 78.72%.

Conclusions: It is concluded that vitamin C has significant effect on increasing testosteron level and sperm quality in gentamicin-induced wistar rats.


Agarwal A, Prabakaran SA and Said TM. Prevention of oxidative stress injury to sperm. J Androl. 2005;26:654-60.

Tanagho, Emil A, Jack W, Mc Aninch. Smith’s general urology. New York. The Mc. Graw-Hill Companies; 2008.

Angulo C, Maldonado R, Pulgar E, Mancilla H, Cardova A, Villarroel F, et al. Vitamin C and oxidative stress in the seminiferus epithelium. Biol Res. 2011;44 (2):169-80.

Istiantoro YH, dan Gan VGH. Penicillin, Cefalosporin and Antibiotics Batalaktam in pharmakologi and therapy. 5th ed. Editor Sulistia G. Ganiswara. Jakarta; 2007.

Khaki A, Ghaffari MN, Fathiazad F, Hossinchi J. Ultra-structural study of gentamicin and ofloxacin effect on testis tissue in rats: light and transmision electron microscopy. Afr J Pharm Pharma. 2009;3(4):12-34.

Winarsi H. Natural antioxidants and free radicals. Yogyakarta: Kanisius. 2007;5:122-204.

Champe, Pamela C, Harvey. Biochemistry. Jakarta. EGC; 2010.

Almasdy D, Deswinar, Helen. Evaluation of antibiotic use in a government hospital in the city of Padang. Proceeding of National Seminar Development of Police Science Clinic. 2013;2339-2592:7-15.

Keffer JC, Agarwal A, Sabanegh E. Role of antioxidant in the treatment of male infertility. Glickman Urological and Kidney Institute, Clevelnd Clinic, Cleveland, Ohio, USA. International J Urol. 2009;16:449-57.

Evans WJ. Vitamin E, vitamin C, and exercise. The Am J Clin Nutri. 2000 Aug 1;72(2):647S-52S.

Laurence R, Gesquiere, Patrick O, Onyango, Susan C, Alberts, Altman J. Endocrinology of year-round reproduction in a highly seasonal habitat: environmental variability in testosterone and glucocorticoids in baboon males. Am J Physical Anthropol. 2011;176:144:69.

Akondi BR, Akula A, Challa RS. Protective effects of rutin and naringenin on gentamicin induced testikule oxidative stress. College of Pharmaceutical Sciences, Division of Pharmacology, Andhara University, Visakhapatnam, India. Euro J General Med. 2011;8(1):57-64.

Marrai, Darawany E, Fadiel AA, Abdel A. Reproductive performance traits as effected by heat stress and its alleviation in sheep. Faculty of Agriculture Zagazig University. 2008;8:209-34.

Greenspan AC, Hall JE. Text book of Medical Physiology. USA. Saunder Elsevier; 2014.

Kilarkaje N. An aminoglycoside antibiotic gentamicin induces oxidative stress, reduces-antioxidant reserve, and impairs spermatogenesis rats. J Toxicol SCI. 2007;33(1):85-96.

Cruz, Lume C, Vieira JS, Nunes A, Castro A. Oxidative stress markers: can they be used to evaluate human sperm quality. Turky J Urol. 2015;41(4):198-207.

WHO. Mother or nothing: the agony of infertily. Bull World Health Organ. 2010;88:881-2.

Favig EM, Foad O. Serum and plasma levels of total and free testosterone and of sex hormone binding globulins in rats growing in the below sea level environtment of the Jordan valley. J Endo. 2009;5(2):1-6.




How to Cite

Rahayu, I., Usman, E., & Reza, M. (2019). Effect of vitamin C on testosterone level, sperm count and sperm morphology in gentamicin-induced Wistar rats. International Journal of Research in Medical Sciences, 7(2), 451–456. https://doi.org/10.18203/2320-6012.ijrms20190352



Original Research Articles