Published: 2019-02-27

Comparison of fasting and non-fasting serum lipid profile in healthy population

K. S. Mandle, Viplav Prashant, Gopal K. Sahu


Background: Serum lipids are routinely used for the assessment of cardiovascular risk. The test is usually performed under fasting condition. However, recently non-fasting lipid profile is also measured in certain cases. The present study was intended to estimate the concentration of total cholesterol (TC), triglyceride (TG), HDL cholesterol (HDL-C), LDL cholesterol (LDL-C) and VLDL cholesterol (VLDL-C) in fasting as well as non-fasting blood samples of a group of normal male and female subjects.

Methods: The study recruited 50 normal healthy male and female subjects within the age group of 12-48 years. The concentration of TC, TG, HDL-C, LDL-C and VLDL-C in serum of these subjects were quantitatively determined using the commercially available kits based on CHOD/POD method.

Results: The mean concentration of non-fasting TC, TG, HDL-C, LDL-C and VLDL-C was found to be 141.20 mg/dl, 132.20 mg/dl, 50.39 mg/dl, 64.30 mg/dl, 26.44 mg/dl respectively. On the other hand, the mean concentration of fasting TC, TG, HDL-C, LDL-C and VLDL-C was found to be 112.37 mg/dl, 100.90 mg/dl, 38.59 mg/dl, 53.59 mg/dl and 20.18 mg/dl respectively. The concentration of fasting lipid profile parameters was significantly low from the respective parameters of non-fasting lipid profile.

Conclusions: The present study reveals that there was an increase in the levels of TC, TG, HDL-C, LDL-C, and VLDL-C in non-fasting state compared to the fasting state. Measuring the lipid profile parameters under non-fasting state cannot be usually considered for assessment of cardiovascular risk and for other clinical purposes.


Cholesterol, Fasting, Lipid profile, Non-fasting, Triglyceride

Full Text:



Fahy E, Subramaniam S, Murphy RC, Nishijima M, Raetz CR, Shimizu T, et al. Update of the LIPID MAPS comprehensive classification system for lipids. J Lipid Res. 2009;50(Supplement):S9-14.

Sidhu D, Naugler C. Fasting time and lipid levels in a community-based population: a cross-sectional study. Arch Inter Med. 2012;172(22):1707-10.

Campos H, Khoo C, Sacks FM. Diurnal and acute patterns of postprandial apolipoprotein B-48 in VLDL, IDL, and LDL from normolipidemic humans. Atherosclerosis. 2005;181(2):345-51.

De Backer G, Ambrosioni E, Borch-Johnsen K, Brotons C, Cifkova R, Dallongeville J, et al. European guidelines on cardiovascular disease prevention in clinical practice. third joint task force of European and other societies on cardiovascular disease prevention in clinical practice (constituted by representatives of eight societies and by invited experts). Atherosclerosis. 2004;173(2):381-91.

Grundy SM. Third report of the national cholesterol education program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (Adult Treatment Panel III) final report. Circulation. 2002; 106:3143-421.

Nordestgaard BG, Langsted A, Freiberg JJ. Nonfasting hyperlipidemia and cardiovascular disease. Current Drug Targets. 2009;10(4):328-35.

Wattanasuwan N, Khan IA, Gowda RM, Vasavada BC, Sacchi TJ. Effect of acute myocardial infarction on cholesterol ratios. Chest. 2001;120(4):1196-9.

Mora S, Rifai N, Buring JE, Ridker PM. Fasting compared with nonfasting lipids and apolipoproteins for predicting incident cardiovascular events. Circulation. 2008;118(10):993.

Bansal S, Buring JE, Rifai N, Mora S, Sacks FM, Ridker PM. Fasting compared with non-fasting triglycerides and risk of cardiovascular events in women. JAMA. 2007;298(3):309-16.

Nordestgaard BG, Benn M, Schnohr P, Tybjærg-Hansen A. Non-fasting triglycerides and risk of myocardial infarction, ischemic heart disease and death in men and women. JAMA. 2007;298(3):299-308.

Sarvar R, Danesh J, Eriksdottir J. Triglycerides and the risk of heart disease. 10, 158 incident cases among 262, 525 participants in 29 western population studies. Circulation. 2007;115(4):450-8.