Study of fungal pathogen in onycomycoses patients attending the teaching hospital

Authors

  • Archana Wankhade Department of Microbiology, Chandulal Chandrakar Memorial Medical College, Kachandur, Durg, Chhattisgarh, India
  • Dnyaneshwari Ghadage Department of Microbiology, Smt Kashibai Navale Medical College and Hospital, Narhe, Pune, Maharashtra, India
  • Arvind Bhore Department of Microbiology, Smt Kashibai Navale Medical College and Hospital, Narhe, Pune, Maharashtra, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20164201

Keywords:

Dermatophytes, Nail pathogens, Onychomycoses, Superficial mycoses

Abstract

Background: Onychomycosis is commonly caused by dermatophytic group of fungi. Non dermatophytic group of fungi are rarely isolated from nail Onychomycosis due to non dermatophytic molds Cladosporium spp. Aim of the study was to isolate and identify fungal agents from nail clipping of patient clinically having onycomycoses.

Methods: Clinical samples from 226 clinically suspected onychomycotic patients were processed in the microbiology laboratory. These nail samples were subjected to 40% KOH examination .Samples were cultured on SDA and SDA chloramphenicol and cycloheximide for isolation of causative agents. These were identified microscopically and macroscopically.

Results: Total 35.8% samples were positive for fungus and showed fungal elements in 40% KOH mount amongst which 53.08% showed culture positivity.

Conclusions: Dermatophytes are the predominant group of fungi causing onychomycoses. These studies signify the importance of mycological examination in the diagnosis of nail infections for further effective management.

Metrics

Metrics Loading ...

References

Chander J. Textbook of Medical Mycology. Mehta publisher. 3rd edition. 2009.

Weitzman I, Summerbell RC. The dermatophytes. Clin Microbiol Rev. 1995;8:240-59.

Kaur R, Kashyap B, Bhalla P. A five-year survey of onychomycosis in New Delhi, India: Epidemiological and laboratory aspects. Indian J Dermatol. 2007;52:39-42.

Kaur R, Kashyap B, Bhalla P. Onychomycosis - epidemiology, diagnosis and management. Indian J Med Microbiol. 2008;26:108-16.

Sobhanadri C, Rao DT, Babu KS. Clinical and mycological study of superficial fungal infections at government hospital: Guntur and their response to treatment with Hamycin, Dermostatin and dermamycin. Indian J Dermatol Venereol Leprol. 1970;36:209-14.

Veer P, Patwardhan NS, Damle AS. Study of onychomycosis: Prevailing fungi and pattern of infection. Indian J Med Microbiol. 2007;25:53-6.

Maraki S, Nioti E, Mantadakis E, Tselentis Y. A 7-year survey of dermatophytoses in Crete Greece. Mycoses. 2007;50(6):481-4.

Jha BK, Mahadevamurthy S, Sudisha J, Bora A. Isolation, Identification and Antifungal Susceptibility Test of Dermatophytes from the Patients with Onychomycosis in Central Nepal American Journal of Dermatology and Venereology. 2015;4(3):30-6.

Scher RK. Onychomycosis is more common than a cosmetic problem. Br J Dermatol. 1994;130;159.

Milos D, Pavlovic MD, Bulajic N. Great toenail onychomycosis caused by Syncephallastrum racemosum. Dermatol Online J. 2006;12:7.

Agarwalla A, Agrawal S, Khanal B. Onychomycosis in eastern Nepal. Nepal Med Coll J. 2006;8:1-7.

Farwa U, Abbasi SA, Mirza IA, Amjad A, Ikram A, Malik N, et al. Non-Dermatophyte Moulds as Pathogens of Onychomycosis. J Colle Phys Surg Pak. 2011; 21(10):597-600.

Malik NA, Raza N, Nasiruddin. Non-dermatophyte moulds and yeasts ascausative agents in onychomycosis. J Pak Asso Derm. 2009;19:74-8.

Tisti A, Piraccini BM, Lorenzi S. onychomycosis caused by non-dermatophytes: Clinical features and response to treatment of 59 cases. J Am Acad Dermatol. 2000;42:217-24.

Doddamani PV, Harshan KH, Kanta RC, Gangane R, Sunil KB. Isolation, Identification and Prevelance of Dermatophytes in Tertairy Care Hospital in Gulbarga District. People’s J Scientific Research. 2013;6(2).

Garcia-Martos P, Guarro J, Gené J, Linares M, Ortoneda M. Onychomycosis caused by Aspergillus sclerotiorum. J Mycol Med. 2001;11:222-4.

Kristensen L, Stenderup J, Otkjaer A. Onychomycosis due to Aspergillus tamarii in a 3-year-old boy. Acta Derm Venereol. 2005;85:261-2.

Takahata Y, Hiruma M, Sugita T, Muto M. A case of onychomycosis due to Aspergillus sydowii diagnosed using DNA sequence analysis. Mycoses 2008;51:170-3.

Escobar ML, Carmona-Fonseca J. Onychomycosis by common non-dermatophyte moulds. Rev Iberoam Micol. 2003;20:6-10.

Zotti M, Machetti M, Perotti M, Barabino G, Persi A. A new species, Aspergillus persii, as an agent of onychomycosis. Med Mycol. 2010;48:656-60.

Zotti M, Machetti M, Persi A, Barabino G, Parodi, A. Onychomycosis: First case due to aspergillus nomius. Acta Dermato-Venereologica. 2011;91(5):591-2.

Pontes ZB, Lima Ede O, Oliveira NM, Das Santosh JP, Ramos AL, Carvalho MF. Onychomycosis in Pessoa city, Brazil. Rev Argent Microbiol. 2012;34:95.

Jayatilake, J.A.M.S., Tilakaratne, W.M. & Panagoda, G.J. Mycopathologia. 2009;168:165.

Downloads

Published

2016-12-16

How to Cite

Wankhade, A., Ghadage, D., & Bhore, A. (2016). Study of fungal pathogen in onycomycoses patients attending the teaching hospital. International Journal of Research in Medical Sciences, 4(12), 5314–5318. https://doi.org/10.18203/2320-6012.ijrms20164201

Issue

Section

Original Research Articles