Published: 2019-02-27

Evaluation of the analgesic activity of the water soluble extract of stem of Tinospora cordifolia in experimentally induced pain in albino rats

Sumanlata ., Akanksha Suman, Rajeev Kumar Sharma, Meenakshi Jindal, Adnan Khan


Background: Pain and pyrexia are the warning signals, primarily protective in nature, that cause discomfort and suffering and may even be unbearable and incapacitating. The modern drugs (like opioids, NSAIDs, corticosteroids) currently used for the management of pain, fever and inflammatory conditions, present with many known adverse effects. Tinospora cordifolia known as Giloe, widely used in folk medicine due to its property to cure a number of diseases. Hence the present study was undertaken to explore the analgesic activity of water-soluble extract of stem of T. cordifolia in albino rats in experimentally induced pain.

Methods: Present study was done in the department of pharmacology, albino rats were used to study the analgesic activity of T. cordifolia aqueous extract at the dose of 1.25g/kg,2.5g/kg and 5g/kg p.o. Various methods like Eddy’s hot plate, tail flick test and acetic acid induced writhing were used for the anti- nociceptive study.

Results: In Eddy’s hot plate and tail flick test an increase in reaction time was observed with peak effect at 90min. Results were similar to the standard drug Tramadol in acetic acid induced writhing increase in time of onset, decrease in number and duration of writhing was observed.

Conclusions: Aqueous extract of T. cordifolia was effective in all the three models of pain suggesting its possible action by central and peripheral mechanisms. Activity of T. cordifolia can be attributed to various phytoconstituents viz. protoberberine alkaloids, terpenoids, glycosides and polysaccharides. It can be developed as potent analgesic agent in future.


Analgesic, Anti-inflammatory, Aqueous extract, Albino rats, Tinospora cordifolia, Writhing

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Woolf CJ. Somatic pain-pathogenesis and prevention. Br J Anaesth. 1995;75:169-76.

Boron WR. Pain and temperature. In: Barrett K, Brooks H, Barman SB, Barman S, editors. Ganong’s Review of Medical Physiology. 23rd ed. New Delhi: Tata McGraw-Hill; 2010:168.

Determination of Nutritional value and Mineral

elements of some important medicinal plants from Western part of India. J Med Plants. 2009;8(5):79-88.

Singh J, Sinha K, Sharma A, Mishra NP, Khanuja SP. Traditional uses of Tinospora cordifolia (Guduchi). J Med Aromat Plant Sci. 2003 Jan;25:748-51.

Bhandari C. Vanaushadhi Chandrodaya. 1st ed. Varanasi: Chaukhamba Sanskrit Sansthan; 2006:86.

Panchabhai TS UPK, Rege NN. Validation of Therapeutic Claims of Tinospora cordifolia: A Review. Phytother Res. 2008; 22:425-41.

Srivastav P. Tinospora cordifolia (Amrita)-A miracle herb and lifeline to many diseases. Int J Med Aromatic Plants. 2011;1(2):57-61.

Eddy NB, Leimbach D. Synthetic analgesics. II. Dithienylbutenyl-and dithienylbutylamines. J Pharmacol Exp Therap. 1953 Mar 1;107(3):385-93.

Bikash M, Ajay P. Practical Manual of Experimental and Clinical Pharmacology. 1st Ed. 2010:201-2.

Koster R, Anderson M, De Beer EJ. Acetic acid for analgesic screening. Fed Proc. 1959;18:412.

The Wealth of India. Raw Materials; Publication and Information Directorate, Council of Scientific and Industrial Research: New Delhi. 1982;10:252.

Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC, Chemistry and medical properties of Tinospora Cordifolia (Guduchi) Indian J Pharmacol. 2003; 35:83-91.

Siddalingappa C, Rajesh T, Kudagi B, Krishnakanth K, Sujith TR. Evaluation of analgesic and antiinflammatory activites of Tinospora cordifolia in rodents. Int J Basic Med Sci. 2012;2(6):306-11.

Hossain MM, Hasan SR, Akter R, Islam MN, Rashid MJ, Saha MR, et al. Evaluation of analgesic and neuropharmacological properties of the aerial part of Tinospora cordifolia Miers. in mice. Stamford J Pharma Sci. 2009;2(2):31-7.

Upadhyay AK, Kumar K, Kumar A, Mishra HS. Tinospora cordifolia (Willd.) Hook. f. and Thoms.(Guduchi)0-validation of the Ayurvedic pharmacology through experimental and clinical studies. Int J Ayurveda Res. 2010 Apr;1(2):112.

Panchabhai TS UPK, Rege NN. Validation of therapeutic claims of Tinospora cordifolia: a review. Phytother Res. 2008;22:425-41.

Kapil A, Sharma S. Immunopotenting compounds from Tinospora cordifolia. J Ehnopharmacol. 1997;58:89-95.

Stanely M, Prince P, Menon VP. Hypoglycaemic and hypolipidaemic action of alcohol extract of Tinospora cordifolia roots in chemical induced diabetes in rats. Phytother Res. 2003;17:410-3.