DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20191312

Evaluation of the activity of hen eggshell graft in experimentally induced mandibular defects in rabbits: pilot study

Ahmad Al Anashar, Ali Daoud

Abstract


Background: For the treatment of the cranial and maxillofacial bone defects autogenic and/or allogenic bone grafts and alloplastic materials have been used for a long time. In the last decade, avian eggshell powder has been suggested as a bone substitute candidate in reconstructive surgery. The purpose of this study was to investigate the beneficial effects of particulate hen eggshell grafting on the healing of experimentally induced mandibular defects.

Methods: The study was conducted on 10 adult local rabbits. Two cavities of 4mm diameter have been created in the lateral surface of the mandible. One of the cavities was filled with particulate hen eggshell graft (test one), while the other hole was left for normal healing (control one). On the 8 weeks, the rabbits have been sacrificed and defective regions have been extracted.

Results: All animals showed normal wound healing. No foreign body reaction was observed, hen eggshell grafts were resorbed, integrated with bone at 8 weeks.  There were no differences between control and chicken eggshell groups in new bone formation. At 8 weeks, histological analysis of specimens showed different amounts of woven bone contained osteocytes and covered by lining of osteoblasts.

Conclusions: Within the limitations of this study, it was concluded that hen eggshell powder is a worth-while bone substitute because it is a safe, cheap, and easily available material.


Keywords


Bone defects, Bone graft material, Eggshell powder

Full Text:

PDF

References


Kim SG, Kim HK, Lim SC. (Combined implantation of particulate dentine, plaster of Paris, and a bone xenograft (Bio-Oss) for bone regeneration in rats). J Craniomaxillofac Surg. 2001;29:282-8.

Kim SG, Kim WK, Park JC, Kim HJ. A comparative study of osseointegration of Avana implants in a demineralized freeze dried bone alone or with platelet-rich plasma). J Oral Maxillofac Surg. 2002;60:1018-25.

Kurz LT, Garfin SR, Booth JR. (Harvesting autogenous iliac bone grafts: a review of complications and techniques). Spine (Phila Pa 1976). 1989;14(12):1324-31.

Coburn JC, Pandit A. (Development of naturally-derived biomaterials and optimization of their biomechanical properties). Topics Tissue Eng. 2007;3:1-32.

Dupoirieux L, Neves M, Pourquier D. Comparison of pericranium and eggshell as space-fillers used in combination with guided bone regeneration: an experimental study. J Oral Maxillofac Surg. 2000;58:40-6.

Bodner L. Effect of decalcified freeze-dried bone allograft on the healing of jaw defects after cyst enucleation. J Oral Maxillofac Surg. 1996;54:1282-6.

Bauer TW, Muschler GF. Bone graft materials. Clin Orthop. 2000;371:10-27.

Dupoirieux L, Pourquier D, Picot MC, Neves M. Comparative study of three different membranes for guided bone regeneration of rat cranial defects. Int J Oral Maxillofac Surg. 2001;30:58-62.

Dupoirieux L, Pourquier D, Picot MC, Neves M. The effect of pentosan polysulphate on bone healing of rat cranial defects. J Craniomaxillofac Surg. 1999;27:314-20.

Dupoirieux L, Pourquier D, Souyris F. Powdered eggshell: A pilot study on a new bone substitute for use in maxillofacial surgery. J Craniomaxillofac Surg. 1995;23:187-94.

Dupoirieux L. Ostrich eggshell as a bone substitute: A preliminary report of its biological behaviour in animals-A possibility in facial reconstructive surgery. British J Oral Maxillofac Surg. 1999;37:467-71.

Feng QL, Zhu X, Li HD, Kim TN. Crystal orientation in ostrich eggshells. J Crystal Growth. 2001;233:548-54.

Durmus E, Celik I, Aydin MF, Yildirim G, Sur E. Evaluation of the biocompatibility and osteoproductive activity of ostrich eggshell powder in experimentally induced calvarial defects in rabbits. J Biomed Mater Res B Appl Biomater. 2008;86:82-9.

Dupoirieux L, Pourquier D, Picot MC, et al. Resorption kinetics of eggshell: an in vivo study. J Craniofac Surg. 2001;12:53.

Baliga M, Davies P, Dupoirieux L. Powdered eggshell in the repair of cystic cavities of the jaw. Rev Stomatol Chir Maxillofac. 1998;99:86-8.

Durmus E, Celik I, Ozturk A, Ozkan Y, Aydin MF. Evaluation of the potential beneficial effects of ostrich eggshell combined with eggshell membranes in healing of cranial defects in rabbits. J Int Med Res. 2003;31:223-30.