Exfoliative cytology of body fluids: a study from provincial hospital of Jammu region, India
DOI:
https://doi.org/10.18203/2320-6012.ijrms20160720Keywords:
Adenocarcinoma, Body fluids, Effusion, Exfoliative cytology, ExudateAbstract
Background: Cytological study of body fluids is an inexpensive and simple procedure useful in making diagnosis regarding etiology, understanding course of disease and to monitor the response to therapy. It has helped in staging and prognosis of the malignant tumours. The present study aims to understand cytology of various aspirated body fluids, categorize effusions into non neoplastic and neoplastic type with further classification of aspirated fluid as exudate and transudate.
Methods: A total of 185 cases of aspirated fluids coming to Pathology Department of Government Hospital Gandhi Nagar for analysis were studied over a period of one year i.e. from Jan 2015 to Dec 2015.The fluid received was centrifuged and the remaining sediment was transferred with the help of pipette onto two glass slides and spread evenly. One was stained with Giemsa and other was stained with Papanicolaou stain. Leishman stain was done in suspicious cases of CSF. Improved Neubauer counting chamber was used for the cell counts.
Results: Peritoneal fluid was most common aspirated fluid (49.7%) with overall male to female ratio was 1:1.6. Maximum cases belonged to age group of 30-40 years. Only (5.4%) 10 cases were neoplastic. Among malignant effusions maximum (60%) were adenocarcinoma, followed by squamous cell carcinoma (20%).
Conclusions: Exfoliative fluid cytology is rapid and effective method for diagnosis, evaluation and it further guides us in clinical decision making regarding management of underlying pathology.
References
Reagan JW. Exfoliative Cytology of Pleural, Peritoneal and Pericardial Fluids. Cytology series. The Institute of Pathology, Western Reserve University and University Hospitals, Cleveland. Ohio.
Nguyen G. Essentials of fluid cytology. 2009:9-71.
Nathan NA, Narayan E, Smith MM, Horn MJ. Cell block cytology-Improved preparation and its efficacy in diagnostic cytology. Am J Clin Pathol. 2000;114:599-606.
Shulbha VS, Dayananda B. Cytology of body fluids- an aid to primary diagnosis. Indian Journal of Pathol Oncol. 2015;2(2):81-3.
Pradhan SB, Pradhan B, Dali S. Cytology of body fluids from different sites: an approach for early diagnosis of malignancy. J Nepal Med assoc. 2006;45(164):353-6.
Joshi A, Mahajan N, Karmakar PJ. Diagnostic utility of various techniques used in body fluid cytology. J Dent Med Sci. 2014;13(1):13-8.
Kumavat PV, Kulkarni MP, Sulhyan KR. Cytological study of effusions. Indian Med Gazette. 2013:306-13.
Naylor B. Peritoneal fluids. In: Bibbo M. Comprehensive cytophathology. 1st Ed. Philedelphia: WB Saunders. 1991;551-621.
Gieisinger KR, Stanely MW, Raab SS, Silverman JF, Abati A. In: Natasha A. Effusions. Modern Cytopathology. 2nd Ed. Philedelphia: Elsevier. 2004;258-259.
Kinni SR. Serous effusions. In: Mtichell W. Colour atlas of Differential Diagnosis in exfoliative and aspiration cytopathology. 1st Ed. USA: Williams & Wilkins. 1999;119-42.
Kushwah R, Shashilaka P, Hiremath S, Basavaraj HG. Cells in pleural fluid and their value in differential diagnosis. J Cytol. 2008;25(4):138-43.
Wong JW, Pitlik D, Abdul Karim FW. Cytology of pleural, peritoneal and pericardial fluids in children. A 40 year summary. Acta Cytol. 1997;41(2):467-73.
Ehya H. Effusion cytology. Clinical Lab Med. 1991;11(2):443-67.
Jha R, Shrestha HG, Sayami G, Pradhan SB. Study of effusion cytology in patients with simultaneous malignancy and ascitis. Kathmandu Uni Med J. 2006;16(4):483-7.
Sears D, Hajdu S. The cytologic diagnosis of malignant neoplasms in pleural and peritoneal effusions. Acta Cytolol. 1987;31(2):85-97.
