DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20192875

Association between carbohydrate consumption with telomere length based on plasma malondialdehyde in Minangkabau male

Weni F. Nazulis, Delmi Sulastri, Yuniar Lestari

Abstract


Background: Life expectancy was one indicator of human development index in the health sector, one of which was affected by the telomere shortening process. There were many factors that cause shortening of telomere, including an imbalance of carbohydrate consumption then oxidative stress. The study aimed to examine the association between carbohydrate and carbohydrate simplex (sucrose) consumption with telomere length based on plasma malondialdehyde in Minangkabau male.

Methods: The study was cross-sectional with 97 samples from male civil servant, Minangkabau ethnic, 40-50 years old in Padang City. Carbohydrate and sucrose consumptions were obtained through semiquantitative food frequency questionnaire, plasma malondialdehyde examination with thiobarbituric acid test and telomere length measured by quantitative polymerase chain reaction using O'Challagan and Fenech method.

Results: The result showed mean of telomere length was 550.43±188.47 bp; mean of carbohydrate consumption 1280.97±433.57 kcal or 48.80±16.52%; mean of sucrose consumption 179.40±126.61 kcal or 6.83±4.82% and mean plasma malondialdehyde 66.91±13.93 nmol/ml. The association of carbohydrate consumption with telomere length based on plasma malondialdehyde was obtained p-value = 0.714, 0.908 and 0.903. The relationship of sucrose consumption with telomere length based on malondialdehyde was obtained p-value=0,667 and 1,000. Differences in mean telomere length in various categories of carbohydrate and sucrose consumption obtained p-value = 0.547 and 0.559.

Conclusions: There was no significant association between carbohydrate and carbohydrate simplex (sucrose) consumption with telomere length based on plasma malondialdehyde of Minangkabau male; and no significant difference in telomere length in different categories of carbohydrate consumption and different sucrose consumption.


Keywords


Carbohydrate and Sucrose consumption, MDA levels, Telomere length

Full Text:

PDF

References


Ministry of Health, Republic of Indonesia. Basic Health Research, 2010. Jakarta; 2010a.

Pangkahila W. Stay Young, Healthy and Quality. Kompas Media Nusantara: Jakarta; 2017.

Hiyama E, dan Hiyama K. Telomere and Telomerase in Stem Cell. British J Cancer. 2007;96(7):1-7.

Cao K, Blair CD, Faddah DA, dan Al E. Progerin and telomer dysfunction collaborate to trigger celluler senescence in normal human fibroblast. The J Clin Investigation. 2011;121(7):2833-44.

Almatsier S. Basic Principles of Nutrition. Gramedia Pustaka Utama: Jakarta; 2013.

Ahmed R. Is there a balance between oxidative stress and antioxidant defense system during development? Medical J Islamic World Academy Science. 2005;15(2):55-63.

Donne ID, Rossi R, Colombo R, dan Al E. Biomarkers of oxidative damage in human disease. American Assoc Clin Chem. 2006;52(4):601-23.

Salpea KD, Talmud PJ, Cooper JA, dan Al E. Association of telomere length with type 2 diabetes, oxidative stress and UCP2 gene variation. Atherosclerosis. 2010;209(1):42-50.

Ministry of Health, Republic of Indonesia. Public Health Development Index. Jakarta; 2010b.

Kark JD, Goldberger N, Kimura M, Sinnreich R. dan Aviv A. Energy intake and leukocyte telomere length in young adults. Ame J Clin Nutrition. 2012;95(2):479-87.

Leung CW, Laraia BA, Needham B, dan Al E. Soda and cell aging: association between sugar-sweetened beverage consumption and leukocyte telomere length in healthy adults from the national health and nutrition examination surveys. American J Pub Health. 2014;104(12):2425-31.

Ayala A, Munoz M, dan Arguelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine Cellular Longevity; 2014.

Palmieri D, Cafueri G, Mongelli F, Pezzolo A, Pistoia V, Palombo D. Telomere shortening and increased oxidative stress are restricted to venous tissue in patients with varicose veins: A merely local disease?. Vascular Medicine 2014;19(2):125-30.

Biovision. Lipid Peroxidation (MDA) Colorimetric/ Fluorometric Assay Kit; 2016.

Mustofa MS. Telomere shortening in people with diabetes mellitus. YARSI Medical J. 2015;23(3):197-211.

Shammas MA. Telomeres, lifestyle, cancer and aging. HHS Public Access. 2011;14(1):28-34.

O’Callaghan NJ, dan Fenech M. A quantitative pcr method for measuring absolute telomere length. Biological Procedurs Online. 2011;13(3).

Nettleton JA, Diez-Roux A, Jenny NS, Fitzpatrick AL, dan David R Jacobs J. Dietary patterns, food groups, and telomere length in the Multi-Ethnic Study of Atherosclerosis (MESA). The American J Clin Nutrition. 2008;88(5):1405-12.

Freitas-Simoes TM, Ros E, dan Sala-Vila A. Nutrients, foods, dietary patterns and telomere length : Update of epidemiological studies and randomized trials. Meta Clin Experimental. 2016;65:406-15.

Lee J, Jun N, Yoon D, Shin C, dan Baik I. Association between dietary patterns in the remote past and telomere length. European J Clin Nutrition. 2015;69(9):1048-52.

Zhou M, Zhu L, Cui X, Feng L, Zhao X, He S, et al. Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance : a chinese population study. Nutrition J. 2016;15(39).