Anti-inflammatory effects of topical lactobacillus acidophilus and antibiotic in wound repair on the basis of microscopic parameters: a comparative study in rats
DOI:
https://doi.org/10.18203/2320-6012.ijrms20193392Keywords:
Albino rats, Anti-inflammatory, Lactobacillus acidophilus Antibiotic, WoundAbstract
Background: Wound treatment still a task for medical professionals, according to the time advancement. We need to elaborate further new interventions to cope up this common factor of community. The objective of the study was to assess the anti-inflammatory topical role of Lactobacillus acidophilus with antibiotic in wound repair of rats based on of microscopic parameters. This comparative study was conducted in the Department of Anatomy Al-Tibri Medical Collage and Hospital Isra University Karachi Campus from December 2018 to April 2019.
Methods: Total 18 male of wistar rats were randomly selected for this study. The study comprised of three topical groups Control, Antibiotic and Lactobacillus acidophilus groups respectively.
Results: The samples were taken from the wound site for the preparation of microscopic slides, to count the numbers of neutrophils, lymphocytes and macrophages for the evaluation of anti-inflammatory process in both groups on day 3 and 7. Data obtained were analyzed by SPSS version 20 by applying One Way ANOVA and post hoc Tukey’s test.
Conclusions: The results were shown potent anti-inflammatory effects of Lactobacillus acidophilus group in comparison with other conventional therapy. The study concludes that the topical application of Lactobacillus acidophilus had remarkable anti-inflammatory effects in wound management.
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References
Siddharthan R, Chapek M, Warren M, Martindale R. Probiotics in prevention of surgical site infections. Surg Infec. 2018;19(8):781-4.
Misic AM, Gardner SE, Grice EA. The wound microbiome: modern approaches to examining the role of microorganisms in impaired chronic wound healing. Adv Wound Care. 2014;3(7):502-10.
Chehoud C, Rafail S, Tyldsley AS, Seykora JT, Lambris JD, Grice EA. Complement modulates the cutaneous microbiome and inflammatory milieu. Proceedings National Acad Sci. 2013;110(37):15061-6.
Rosa DD, Dias MM, Grześkowiak ŁM, Reis SA, Conceição LL, Maria do Carmo GP. Milk kefir: nutritional, microbiological and health benefits. Nutrition Res Rev. 2017;30(1):82-96.
Nole KLB, Yim E, Keri JE. Probiotics and prebiotics in dermatology. J Am Acad Dermatol. 2004;71(4):814-21.
Lise M, Mayer I, Silveira M. Use of probiotics in atopic dermatitis. Revista da Associação Médica Brasileira. 2018;64(11):997-1001.
Charan J, Biswas T. How to calculate sample size for different study designs in medical research?. Ind J psychol med. 2013;35(2):121.
Kumar S, Mahajan BB, Kamra N. Future perspective of probiotics in dermatology: an old wine in new bottle. Dermatol Online J. 2014;20(9).
Barbu AN, Neamtu M, Zahan M, Miresan V. Novel uses of probiotics in human health. Acta Med Transilvanica. 2016;21(3):26-30.
Tsiouris CG, Kelesi M, Vasilopoulos G, Kalemikerakis I, Papageorgiou EG. The efficacy of probiotics as pharmacological treatment of cutaneous wounds: meta-analysis of animal studies. Europe J Pharmaceut Sci. 2017;104:230-9.
Sinha A, Sagar S, Osborne JW. Probiotic Bacteria in Wound Healing; An in-vivo study. Iranian J Biotechnol. 2019: 17.
Han N, Jia L, Guo L, Su Y, Luo Z, Du J, Mei S, Liu Y. Balanced Oral Microbiomes Promoted Wound Healing via Maintaining Mesenchymal Stem Cell Homeostasis. Available at SSRN 3420378. 2019 Jul 15.
Lukic J, Chen V, Strahinic I, Begovic J, Lev‐Tov H, Davis SC, et al. Probiotics or pro‐healers: the role of beneficial bacteria in tissue repair. Wound Repair and Regeneration. 2017;25(6):912-22.
Siddharthan R, Chapek M, Warren M, Martindale R. Probiotics in Prevention of Surgical Site Infections. Surgical infections. 2018;19(8):781-4.
Li Z, Behrens AM, Ginat N, Tzeng SY, Lu X, Sivan S, Langer R, Jaklenec A. Biofilm‐Inspired Encapsulation of Probiotics for the Treatment of Complex Infections. Adv Materials. 2018;30(51):1803925.
Wong VW, Martindale RG, Longaker MT, Gurtner GC. From germ theory to germ therapy: skin microbiota, chronic wounds, and probiotics. Plastic and reconstructive surg. 2013;132(5):854e-61e.
Dharmani P, De Simone C, Chadee K. The probiotic mixture VSL# 3 accelerates gastric ulcer healing by stimulating vascular endothelial growth factor. PLoS One. 2013;8(3):e58671.