Published: 2019-08-27

Neovascularization: topical effects of streptococcus thermophilus and low level laser therapy in treatment of diabetic wound in rats

Devi Kumari, Hina Khan, Asad R. Jiskani, Muhammad Rafique, Muhammad Asif, Vijay Kumar, Syed Maqsood


Background: Diabetic wound is a major socioeconomic debilitating problem in this society. Various treatment options are available but still it requires better treatment option. In diabetes mellitus the oxygenation to the tissues is reduced. In this study effects of low level laser therapy were compared with topical application of Streptococcus thermophilus on diabetic wounds that induces formation of new blood vessel and free radical scavenging system, a comparative study to get better treatment option for diabetic wounds.

Methods: 18 male rats were selected and divided randomly into three groups. Diabetes was induced in all the rats by using the Alloxan monohydrate at a dose of 120mg/kg of the body weight. Group A was treated with normal saline, group B was treated with low level laser therapy and group C was treated with Streptococcus thermophilus topically. Skin tissues were collected on day three and seven, slides were prepared for microscopic examination to observe the new blood vessels formation.

Results: Mean number of new blood vessel formation was observed in group B compared with group A and C. Significant vasculogenesis was seen in group B when treated with Low level laser therapy.

Conclusions: In the group of low level laser therapy new blood vessel formation was seen with better wound healing. It means LLLT provides better oxygenation to the tissues by generation of new blood vessels compared with Streptococcus thermophilus and normal saline.


Blood vessels, Diabetic Wound, Low level laser therapy (LLLT), Streptococcus thermophilus

Full Text:



Basit A, Fawwad A, Siddiqui SA, Baqa K. Current management strategies to target the increasing incidence of diabetes within Pakistan. Diab, Metab Syndr Obes: Targ Ther. 2019;12:85.

Augustin M, Brocatti LK, Rustenbach SJ, Schaüfer I, Herberger K. Cost-of illness of leg ulcers in the community. Int Wound J. 2014;11(3):283-292.

King A, Balaji S, Keswani SG, Crombleholme TM. The role of stem cells in wound angiogenesis. Adv Wound Care (New Rochelle). 2014;3(10):614-25.

Davis FM, Kimball A, Boniakowski A, Gallagher K. Dysfunctional Wound Healing in Diabetic Foot Ulcers: New Crossroads. J Cur Diab Reports. 2018;18(2):1-8.

Diagnosis and Classification of Diabetes Mellitus. American Diabetes Association. Diabetes care. 2013;36(1):67-74.

Seo E, LimJS, JunJB, Choi W, Hong IS, Jun HS. Exendin-4 in combination with adipose-derived stem cells promotes angiogenesis and improves diabetic wound healing. J Transl Med. 2017;15(35):1145-4.

Rocha Júnior AM, Vieira BJ, Andrade LC, Aarestrup FM. Effects of low-level laser therapy on the progress of wound healing in humans: the contribution of in vitro and in vivo experimental studies. J Vasc Bras. 2007 Sep;6(3):257-65.

Calin MA, Coman T, Calin MR. The effect of low level laser therapy on surgical wound healing. Romanian Reports Physics. 2010;62(3):617-27.

Dungel P, Hartinger J, Chaudary S, Slezak P, Hofmann A, Hausner T, et al. Low level light therapy by LED of different wavelength induces angiogenesis and improves ischemic wound healing. Lasers Surg Med. 2014 Dec;46(10):773-80.

Beckmann KH, Meyer-Hamme G, Schröder S. Low level laser therapy for the treatment of diabetic foot ulcers: a critical survey. Evidence-Based Complement Altern Med. 2014:1-9.

Brauer R, Ruigómez A, Downey G, Bate A, Garcia Rodriguez LA, Huerta C,et al. Prevalence of antibiotic use:a comparison across various European health care data sources. Pharmacoepidemiol Drug Safety. 2016 Mar;25:11-20.

Sanders ME, Merenstein D, Merrifield CA, Hutkins R. Probiotics for human use. J Nutrition Bulletin. 2018;43(3):212-25.

Slashinski MJ, McCurdy SA, Achenbaum LS, Whitney SN, McGuire AL. Snake oil, quack medicine, and industrially cultured organisms: Biovalue and the commerciliztion of human micriome research. BMC Med Ethics. 2012;13(28):1-8.

Li S, Shah NP. Characterization, Anti‐Inflammatory and Antiproliferative Activities of Natural and Sulfonated Exo‐Polysaccharides from Streptococcus thermophilus ASCC 1275. J Food Sci. 2016 May;81(5):M1167-76.

Hamzah RU, Lawal AR, Madaki FM, Erukainure OL. Methanolic extract of Celosia argentea var. crista leaves modulates glucose homeostasis and abates oxidative hepatic injury in diabetic rats. Compar Clin Pathol. 2018 Jul 1;27(4):1065-1071.

Kajagar BM, Godhi AS, Pandit A, Khatri S. Efficacy of low level laser therapy on wound healing in patients with chronic diabetic foot ulcers-a randomised control trial. Ind J Surg. 2012;74(5):359-63.

Chu YH, Chen SY, Hsieh YL, Teng YH, Cheng YJ. Low-level laser therapy prevents endothelial cells from TNF-α/cycloheximide-induced apoptosis. Lasers Med Scie. 2018;33(2):279-86.

Xu Y, Lin Y, Gao S, Shen J. Study on mechanism of release oxygen by photo-excited hemoglobin in low-level laser therapy. Lasers Medical Scie. 2018;33(1):135-9.

Wang Y, Li H, Li T, He H, Du X, Zhang X, et al. Cytoprotective effect of Streptococcus thermophilus against oxidative stress mediated by a novel peroxidase (EfeB). J Dairy Scie. 2018;101(8):6955-63.

Dharmani P, De Simone C, Chadee K. The probiotic mixture VSL# 3 accelerates gastric ulcer healing by stimulating vascular endothelial growth factor. PLoS One. 2013 Mar 6;8(3):e58671.

Martignago CC, Oliveira RF, Pires-Oliveira DA, Oliveira PD, Soares CP, Monzani PS, et al. Effect of low-level laser therapy on the gene expression of collagen and vascular endothelial growth factor in a culture of fibroblast cells in mice. Lasers Medical Scie. 2015;30(1):203-8.