Anti-thrombotic effect of Zingiber officinale (ginger) in sprague dawley rats

Authors

  • Kipyegon Shadrack Department of Medical Physiology, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • Alkizim Faraj Department of Medical Physiology, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • Muriithi K. Alex Department of Pharmaceutical Chemistry and Pharmaceutics, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • Ngure Kenneth Department of Public Health and Community Health, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya

DOI:

https://doi.org/10.18203/2320-6012.ijrms20193905

Keywords:

Coagulation, Ginger, Thrombosis, Zingiber officinale

Abstract

Background: The prevalence of thrombotic diseases is rising globally. Presently, stroke and ischemic heart disease account for 25% of all deaths. Use of anti-thrombotic drugs have proven effective in prevention of these ailments but might not be affordable especially in developing countries. They are also associated with undesirable side effects. This study sought to determine the anti-thrombotic effect of ginger since it is affordable, accessible and is widely used as a food enhancer and a medicinal herb.

Methods: The current study employed an in-vivo experimental study design. Three groups Sprague dawley rats (N=5) were given different doses of methanolic extract of ginger for 30 days. Two other groups (N=5) which served as controls received 5% dimethyl sulfoxide and aspirin for the same duration. Measurement of bleeding time, platelet count, prothrombin time, activated partial thromboplastin time and thrombin time was done to assess the anti-thrombotic property.

Results: There was a statistically significant difference in bleeding time (P=0.03) across the groups investigated. There was however no significant difference across the groups in platelet count, prothrombin time, activated partial thromboplastin time and thrombin time (P=˃0.05).

Conclusion: This study demonstrates that methanolic extract of ginger possesses an anti-thrombotic property probably through inhibition of platelet function. Regular consumption of ginger may therefore confer protection against thrombotic diseases.

References

Shakya SR. Medicinal uses of ginger ( Zingiber officinale Roscoe ) improves growth and enhances immunity in aquaculture. Int J Chem Stud. 2016;3(2):83-7.

Al-amin ZM, Thomson M, Al-qattan KK, Peltonen-shalaby R, Ali M. Anti-diabetic and hypolipidaemic properties of ginger ( Zingiber officinale ) in streptozotocin-induced diabetic rats. Brit J Nutr. 2006:96(4):660-6.

Butt MS, Sultan MT. Ginger and its Health Claims : Crit Rev Food Sci Nutr. 2011;51(5):383-93.

Marx W, Mckavanagh D, Mccarthy AL. The Effect of Ginger ( Zingiber officinale ) on Platelet Aggregation : A Systematic Literature Review. Plos One. 2015:1-13.

Versteeg HH, Heemskerk JWM, Levi M, Reitsma PH. New fundamentals in coagulation. Physiol Rev. 2013:93(1):327-58.

Jackson SP. Arterial thrombosis-insidious, unpredictable and deadly. Nat Med. 2011;17(11):1423-36.

Bhatt DL, Fox KA, Hacke W, Berger PB, Black HR, Boden WE, et al. Clopidogrel and Aspirin versus Aspirin Alone for the Prevention of Atherothrombotic Events. N Eng J Med. 2006 Apr 20;354(16):1706-17.

Yeomans ND, Lanas AI, Talley NJ, Thomson AB, Daneshjoo R, Eriksson B, et al. Prevalence and incidence of gastroduodenal ulcers during treatment with vascular protective doses of aspirin. Alim Pharm Ther. 2005:22(9):795-801.

Chowdhury AW, Siddiq A Bin, Islam AEMM, Saha A. Case reports: Clopidogrel Induced Haematological Dyscrasia : A Case Report. Univ Hrt J. 2009;5(2);89-90.

Nevalainen T, Berge E, Gallix P, Jilge B, Melloni E, Thomann P, et al. FELASA guidelines for education of specialists in laboratory animal science ( Category D ) Report of the Federation of Laboratory Animal Science Associations Working Group on Education of Specialists ( Category D ) accepted by the FELASA Board of Management. Lab An Ltd. 1999 Jan 1;33(1):1-5.

Ghoshal K, Bhattacharyya M. Overview of Platelet Physiology : Its Hemostatic and Nonhemostatic Role in Disease Pathogenesis. The Sci Wrld J. 2014;2014.

Thon JN, Italiano JE. Platelets : Production, Morphology and Ultrastructure Platelets: Production ,Morphology and Ultrastructure. 2012;3-22.

Wachowicz B, Morel A, Miller E, Saluk J. The physiology of blood platelets and changes of their biological activities in multiple sclerosis. Act Neurobiol Exp. 2016 Jan 1;76(4):269-81.

Yun S, Sim E, Goh R, Park J, Han J. Platelet Activation : The Mechanisms and Potential Biomarkers. Mol. 2016;2016:10-15.

Hosseinzadegan H, Tafti DK. Thrombosis & Haemostasis: Research Mechanisms of Platelet Activation, Adhesion and Aggregation. Thromb Haemost.2017;2017.

Heemskerk JW, Bevers EM, Lindhout T. Platelet activation and blood coagulation. Thrombosis and haemostasis. 2002;88(08):186-93.

Broos K, Feys HB, Meyer SF De, Vanhoorelbeke K, Deckmyn H. Blood Reviews Platelets at work in primary hemostasis. Blood Rev. 2011;25(4):155-67.

Gawaz M. Role of platelets in coronary thrombosis and reperfusion of ischemic myocardium. J Cardiores. 2004;61(3):498-511.

Prasad SS, Kumar S, Patel K, Dumater C, Vajpeyee SK. To investigate the action of ginger-juice zingiber ( zingiberaceae ) on blood coagulation process . Int. J Pharm Sci Res. 2012;3(7):407-15.

Young H, Liao J, Chang Y, Luo Y. Synergistic Effect of Ginger and Nifedipine on Human Platelet Aggregation : A Study in Hypertensive Patients and Normal Volunteers. Plos One. 2006;34(4):545-51.

Jiang X, Williams KM, Liauw WS, Ammit AJ, Roufogalis BD, Duke CC, et al. Effect of ginkgo and ginger on the pharmacokinetics and pharmacodynamics of warfarin in healthy subjects. Nat Med. 2005 Apr;59(4):425-32.

Butenas S, Mann KG. Blood Coagulation. Lan. 2002 Jan;67(1):3-12.

Dahlbäck B. Blood coagulation. Bioc Mos. 2000 May 6;355(9215):1627-32.

Yang N, Hao J, Zhang D. Antithrombin III and D-dimer levels as indicators of disease severity in patients with hyperlipidaemic or biliary acute pancreatitis. J Int Med Res. 2017 Feb;45(1):147-58.

Ack D. Pro- and anticoagulant properties of factor V in pathogenesis of thrombosis and bleeding disorders. Int J Lab Haem. 2016;38(1):4-11.

Daly C, Qian X, Castanaro C, Pasnikowski E, Jiang X, Thomson BR, et al. Angiopoietins bind thrombomodulin and inhibit its function as a thrombin cofactor. Sci Rep. 2018 Jan 11;8(1):505.

Brass P. Fibrin formation: Action of Calcium. Blo J. 2019;52(4):654-9.

Hoffman M, Iii DMM. A Cell-based Model of Hemostasis. For Internal Educational Purposes Only. Not for Dissemination. Sgh G Stut. 2001;85(06):958-65

Saxena R, Kannan M, Choudhry VP. Laboratory Studies in Coagulation Disorders. Ind J Paeds. 2007;74(7):53-9.

Taj Eldin IM, MA Elmutalib, Hiba A, Hiba F, Thowiba S, Elnazeer I, et al. An in vitro Anticoagulant Effect of Aqueous Extract of Ginger ( Zingiber officinale ) Rhizomes in Blood Samples of Normal Individuals. Am J Res Com. 2016;4(1):112-21.

Ajala OS, Ogunmade S, Adelekan TA, Oyewole KM. Anticoagulant Activity of Ginger ( Zingiber Officinale Roscoe)Nig J Pharm Res. 2017;13(2):167-73.

El-Ghorab AH, Nauman M, Anjum FM, Hussain S, Nadeem M. A comparative study on chemical composition and antioxidant activity of ginger (Zingiber officinale) and cumin (Cuminum cyminum). J agricult food chem. 2010 Jun 30;58(14):8231-7.

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Published

2019-08-27

How to Cite

Shadrack, K., Faraj, A., Alex, M. K., & Kenneth, N. (2019). Anti-thrombotic effect of Zingiber officinale (ginger) in sprague dawley rats. International Journal of Research in Medical Sciences, 7(9), 3239–3245. https://doi.org/10.18203/2320-6012.ijrms20193905

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Original Research Articles