Assessment of acid phosphatase enzyme and influence of potassium iodide on its production in the yeast form of Sporothrix schenckii
Keywords:Acid phosphatase, Potassium iodide, S. schenckii, Yeast
Background: Sporotrichosis is caused by a dimorphic fungal species, Sporothrix schenckii (S. schenckii). The enzyme acid phosphatase is pervasive among yeast and yeast like fungi. It has been studied in various fungi like Aspergillus oryzae, Candida albicans etc. but in S. schenckii little is known about enzyme acid phosphatase. The present study depicts the in-vitro influence of Potassium Iodide (KI) on the enzyme acid phosphatase produced by the S. schenckii (yeast form).
Methods: A master culture was prepared by incorporating the standard strain of S. schenckii in YNB (Yeast Nitrogen Base) medium and was incubated at 37ºC. After preparing the increasing concentrations with KI in YNB medium, 1.0 mL suspension of master culture was inoculated into each bottle and incubated at 37ºC for different time period 6th, 12th, 18th day (early, mid, peak of log period) respectively. After centrifuging, a 5% homogenate was prepared, which was used for acid phosphatase enzyme assay.
Results: The mean acid phosphatase level of control specimen was 20.9±2.01, 50.0±2.25, 45.0±5.10 μg and test specimens was ranged from 14.9±4.89 to 20.2±3.49, 10.2±4.19 to 40.0±6.39 and 10.0±1.81 to 34.7±6.08 μg on day 6, 12 and 18 respectively. The mean value was lower significantly for all the test concentrations as compared to control (p<0.05).
Conclusions: The low activity of the enzyme acid phosphatase indicates that KI has inhibitory effect on the growth of S. schenckii that has led to decrease in the activity of the enzyme.
Bastos de Lima Barros M, de Almeida Paes R, Oliveira Schubach A. Sporothrix schenckii and sporotrichosis. Clin Microbiol Rev. 2011;24:633-54.
Bareja R, Grover PS, Mehra SK. In vitro effect of potassium iodide on growth of Sporothrix schenckii in mycelial form. Sch J App Med Sci. 2015;3(7D):2676-9.
Carlos IZ, Sassá MF, da Graça Sgarbi DB, Placeres MC, Maia DC. Current research on the immune response to experimental sporotrichosis. Mycopathol. 2009;168(1):1-10.
Carlos IZ, da Graca Sgarbi DB, Angluster J, Alviano CS, Silva CL. Detection of cellular immunity with the soluble antigen of the fungus Sporothrix schenckii in the systemic form of the disease. Mycopathol. 1992;117(3):139-44.
Maia DC, Sassá MF, Placeres MC, Carlos IZ. Influence of Th1/Th2 cytokines and nitric oxide in murine systemic infection induced by Sporothrix schenckii. Mycopathol. 2006;161(1):11-9.
Travassors LR. Sporothrix schenckii. In: Szaniszol PJ, Editors. Fungal dimorphism, with emphasis on fungi pathogenic for humans. New York: Plenum Press; 1985:121-163.
Arnold WN, Garrison RG. Kinetic limitations on the trapping of nascent phosphate for cytochemical localization of yeast acid phosphatase. Curr Microbiol. 1981;5(1):57-60.
Hogan LH, Klein BS, Levitz SM. Virulence factors of medically important fungi. Clini Microbiol Rev. 1996;9(4):469-88.
King EJ, Jegatheesan KA. A method for the determination of tartrate-labile, prostatic acid phosphatase in serum. J Clini Pathol. 1959;12(1):85-9.
Nozawa SR, MacCheroni Jr W, Stábeli RG, Thedei Jr G, Rossi A. Purification and properties of pi-repressible acid phosphatases from Aspergillus nidulans. Phytochem. 1998;49(6):1517-23.
Yoshida H, Tamiya N. Acid phosphatases from Fusarium moniliforme. J Biochem. 1971;69(3):525-34.
Yoshida H, Oikawa S, Ikeda M, Reese ET. A novel acid phosphatase excreted by Penicillium funiculosum that hydrolyzes both phosphodiesters and phosphomonoesters with aryl leaving groups. J Biochem. 1989;105(5):794-8.
Nahas E, Terenzi HF, Rossi A. Effect of carbon source and pH on the production and secretion of acid phosphatase (EC 3.1. 3.2) and alkaline phosphatase (EC 3.1. 3.1) in Neurospora Crassa. Microbiol. 1982;128(9):2017-21.
Garrison RG, Arnold WN. Cytochemical localization of acid phosphatases in the dimorphic fungus Sporothrix schenckii. Current Microbiol. 1983;9(5):253-7.
Arnold WN, Mann LC, Sakai KH, Garrison RG, Coleman PD. Acid phosphatases of Sporothrix schenckii. Microbiol. 1986;132(12):3421-32.