A comparative study of efficacy of gabapentin in inflammation induced neuropathic animal pain models with conventional analgesic diclofenac

Arunim Swarup; Ruchika Agarwal; Sunil Malhotra; Abhay Shankar Dube

doi:10.18203/2320-6012.ijrms20161204

Authors

Arunim Swarup Department of Orthopaedics, Subharti Medical College, Meerut, U.P
Ruchika Agarwal Department of Physiology, Subharti Medical College, Meerut, U.P
Sunil Malhotra Department of Orthopaedics, Subharti Medical College, Meerut, U.P
Abhay Shankar Dube Department of Orthopaedics, Subharti Medical College, Meerut, U.P

DOI:

https://doi.org/10.18203/2320-6012.ijrms20161204

Keywords:

Gabapentin, Diclofenac, Nociception, Formalin test

Abstract

Background: Some antiepileptic drugs have been shown to be clinically efficacious in treatment of neuropathic pain and are being used by clinicians. This study is proposed to evaluate the efficacy of these drugs as compared to conventional analgesics.

Methods: Formalin test has been used as the model of acute and chronic inflammatory pain. Formalin has been characterized by the occurrence of two characteristic phases of increased pain sensitivity in rats. The test drugs have been administered orally and scored according to a pain scale. Pain has been quantified by counting the incidence of spontaneous flinches, shakes and jerks of the formalin injected paw. Analgesic response or protection has been indicated if both paws are resting on floor with no obvious favouring of injected paw. Treatment group was compared with appropriate control groups using “student t test”.

Results: Per oral administration of gabapentin produced no any marked effect on early phase response of formalin test but significantly suppressed the late phase response while diclofenac produced significant antinociceptive effect in both phases of formalin test.

Conclusions: Thus we have observed that gabapentin produced antinociception in second phase of formalin test which reflects chronic inflammatory pain.

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References

Merskey H, Albe-Fessard DG, Bonica JJ, Carmon A, Dubner R, Kerr FWL, et al. Pain terms a list with definitions and notes on usage. Pain. 1979;6:249-52.

Pain Free: Modern Drugs and Neuropathic Pain. J Korean Med Science. 2002;22:360-7.

Maizes M, Mccarberg B. Antidepressant and antiepileptic drugs for chronic noncancer pain. Am Fam Physician. 2005;71:483-90.

Assi D, Azim A, Rahman A, Mahran S. Analgesic effects of tramadol-diclofenac combination and their interaction with sycostimulant drugs in mice and rats. Eur J Pharmacol. 1996;312:132-8.

Carrie K, Jones SC. Efficacy of duloxitine, a potent and balanced serotonergic and noradrenergic reuptake inhibitor, in inflammatory and acute pain models in rodents. J Pharmacol Exp Ther. 2005;313:726-32.

Vogel HG, Vogel WGH, editors, Drug discovery and evaluation-pharmacological assays. 2nd ed. New York: Verlog Springer publication; 1996.

Sukriti, Hota D, Pandhi P. Potentiation of antihyperalgesic activity of diclofenac by nimodipine in a formalin model of facial pain in rats. Exp Clin Pharmacol. 2004;26:253-6.

Hernandez GC, Soto VG, Ortiz MI. Pinacidil increases diclofenac antinociception in the formalin test. Proc West Pharmacol Soc. 2005;48:55-8.

Yoon MH, Choi J, Kwak SH. Characteristic of interaction between intratheca gabapentin and either clonidine or neostigmine in the formalin test. Anesth Analg. 2004;98:1374-9.

Carlton SM, Zhou S. Attenuation of formalin induced nociceptive behaviors following local peripheral injection of gabapentin. Pain. 1998;76:201-7.

Heughan CE, Sawynok J. The interaction between gabapentin and amitryptyline in the rat formalin test after systemic administration. Anesth Analg. 2002;94:975-80.

Field MJ, Oles RJ, Lewis AS, McCleary S, Hughes J, Singh L. Gabapentin (neurontin) and s-(+)-3-isobutylgaba represent a novel class of selective antihyperalgesic agents. Br J Pharmacol. 1997;121:1513-22.

Urban MO, Ren K, Park KT, Campbell B, Anker N, Stearns B, et al. Comparison of the antinociceptive profiles of gabapentin and 3 methyl gabapentin in rat models of acute and persistent pain: Implications for mechanism of action. J Pharmacol Exp Ther. 2005;313:1209-16.

Dickenson D, Sullivan AF. Peripheral origins and central modulation of subcutaneous formalin induced activity of rat dorsal horn neurons. Neurosci Lett. 1987;83:207-11.

Field MJ, Hughes J, Singh L. Further evidence for the role of the alpha2 delta subunit of voltage dependent calcium channels in models of neuropathic pain. Br J Pharmacol. 2000;13:282-6.

Cheng JK, Lai YJ, Chen CC, Cheng CR, Chiou LC. Magnesium chloride and ruthenium red attenuate the antiallodynic effect of intrathecal gabapentin in arat model of postoperative pain. Anesthesiology. 2003;98:1472-9.