Haematological profile of sickle cell patients attending tertiary health care centre of southern odisha: a cross sectional study

Authors

  • Samira K. Behera Department of Pathology, SLN Medical College, Koraput, Odisha, India
  • Sonali Kar Department of Pathology, MKCG Medical College, Berhampur, Odisha, India
  • Monali Kar Department of Community Medicine, MKCG Medical College, Berhampur, Odisha, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20195902

Keywords:

Anaemia, Fetal hemoglobin, Hemoglobin variant, High-performance liquid chromatography, Sickle cell disease

Abstract

Background: Sickle Cell Disorder (SCD) is a major health problem in India. After introduction of High-Performance Liquid Chromatography (HPLC) in MKCG Medical College, this study is first of its kind to describe haemoglobin variants of SCD. The aim of the study was to document haematological profile and pattern of haemoglobin variants in SCD patients.

Methods: A Hospital based cross sectional study was conducted in Pathology department, MKCG medical college from October 2018 to May 2019. Sickle cell patients were included and patients in Sickle cell crisis or transfused with blood in last 3 months were excluded. Hematological indices were measured by Sysmex XT 2000i blood analyzer. Quantification of hemoglobin variants was done by HPLC. All data were analyzed using SPSS and Independent t-test was applied.

Results: In this study 100 heterozygous and 116 homozygous cases were reported. In homozygous cases Hb were significantly low and MCV, MCH, RDW-CV were significantly high than heterozygous. Hb level was significantly lower in homozygous children. Hb F was significantly higher in children and homozygous cases. A significant positive correlation was seen between Hb and RBC in both cases.

Conclusions: In homozygous cases moderate anaemia (microcytic hypochromic to normocytic hypochromic) with High Hb F and in heterozygous cases mild anaemia (microcytic hypochromic) dominated the haematological profile. Children were significantly more anaemic than adults in homozygous cases. Anisocytosis was significantly more in homozygous cases and pediatric age group. Average fetal Haemoglobin variant (Hb F) was significantly more in homozygous cases and lower in adult group in both homo and heterozygous cases.

References

Agarwal MB, Mehta BC. Sickle-syndromes: a study of 44 cases from Bombay. Ind Pediatr. 1980 Oct;17(10):793.

Herrick JB. Peculiar elongated and sickle-shaped red blood corpuscles in a case of severe anemia. 1910. Yale J Biol Med. 2001 May;74(3):179.

Lehmann H, Cutbush M. Sickle-cell trait in southern India. Br Med J. 1952 Feb 23;1(4755):404.

Balgir RS, Sharma SK. Distribution of sickle cell hemoglobin in India. IndJ Hemat. 1988;6:1-4.

Balgir RS. Health care strategies, genetic load, and prevention of hemoglobinopathies in tribal communities in India. South Asian Anthropol. 2004;4(2):189-98.

Mohanty D, Mukherjee MB. Sickle cell disease in India. Curr Opinion Hematol. 2002 Mar 1;9(2):117-22.

Balgir RS. Genetic epidemiology of the three predominant abnormal hemoglobins in India. J Assoc Phys Ind. 1996 Jan;44(1):25-8.

Kamble Á, Chaturvedi P. Epidemiology of sickle cell disease in a rural hospital of central India. Ind Pediatr. 2000 Apr 1;37(4):391-6.

Chavda J, Goswami P, Goswami A. Hematological profile of sickle cell disorder in tertiary care hospital. IOSR J Dent Med Sci. 2015;14:51-4.

Pathak K, Kishore S, Shivkumar VB, Gangane N, Sharma S. Study of haemoglobin S percentage and haematological parameters in sickle cell trait. Ind J Pathol Microbiol. 2003 Jul;46(3):420-4.

Khan Y, Thakur AS, Mehta R, Kundu RK, Agnihotram G. Hematological profile of sickle cell disease: a hospital-based study at Cims, Bilaspur, Chhattisgarh. IJABPT. 2010 Aug;1(2):717-21.

Shrikhande AV, Dani AA, Tijare JR, Agrawal AK. Hematological profile of sickle cell disease in central India. Ind J Hematol Blood Trans. 2007 Dec 1;23(3-4):92-8.

Rao SS, Goyal JP, Raghunath SV, Shah VB. Hematological profile of sickle cell disease from South Gujarat, India. Hematol Reports. 2012 May 10;4(2).

Kar BC, Kulozik AE, Sirr S, Satapathy RK, Kulozik M, Serjeant BE, et al. Sickle cell disease in Orissa state, India. Lancet. 1986 Nov 22;328(8517):1198-201.

Hayes RJ, Beckford M, Grandison Y, Mason K, Serjeant BE, Serjeant GR. The haematology of steady state homozygous sickle cell disease: frequency distributions, variation with age and sex, longitudinal observations. Br J Haematol. 1985 Feb;59(2):369-82.

Downloads

Published

2019-12-25

How to Cite

Behera, S. K., Kar, S., & Kar, M. (2019). Haematological profile of sickle cell patients attending tertiary health care centre of southern odisha: a cross sectional study. International Journal of Research in Medical Sciences, 8(1), 170–176. https://doi.org/10.18203/2320-6012.ijrms20195902

Issue

Section

Original Research Articles