Effects of aloe vera extracted on liver and kidney function changes induced by hydrogen peroxide in rats
Keywords:Aloe vera, Liver and kidney functions, Lipid profiles, Rats
Background: Aloe vera (Aloes) is a member of the Liliaceae family that is used as herbal medicine in many cultures for several purposes. The present study was designed to investigate the role of Aloe vera leaf gel extracts on lipid profiles and liver and kidney functions in rats.
Methods: In this experimental investigation, a total of 20 healthy rats were divided into four following groups. Group I fed with normal diet and water. Group II administrated by 1% hydrogen peroxide with drinking water in a dark bottle prepared daily. Group III administrated with 5 ml of aloe vera oil added to 25 grams of their ratio for each rat (25 ml oil/125 g) also prepared daily with normal drinking water. Group IV also administrated with 5 ml of aloe vera oil added to 25 grams of their ratio with drinking water that contains 1% hydrogen peroxide in a dark bottle. The rats in all four groups fed for 21 days.
Results: The subjects who were included in H2O2 had significantly higher concentrations of TG (146.79 vs. 73.09 mg/dL; p<0.001), cholesterol (123.60 vs. 68.90 mg/dL; p=0.001), and lower concentration of HDL (5.79 vs. 7.53 mg/dL; p<0.001) compared to the control group. While, the subjects in Aloe Vera group had lower concentration of cholesterol (55.90 vs. 68.90 mg/dL; p=0.004), and higher level of HDL (9.22 vs. 7.53 mg/dL; p<0.001). The subjects in the H2O2 had significantly higher concentrations of AST (76.64 vs. 30.04; p<0.001), ALT (64.94 vs. 23.38; p<0.001), urea (59.68 vs. 37.10; p=0.003), uric acid (0.92 vs. 0.59; p<0.001). Whereas, the subjects in Aloe Vera had substantially lower levels of AST (18.76 vs. 30.04; p=0.008).
Conclusions: The present study showed that aloe vera gel extract is effective to improve the lipid profile and liver and kidney function.
Rajasekaran S, Ravi K, Sivagnanam K, Subramanian S. Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clin Exper Pharmacol Physiol. 2006;33(3):232-7.
Anilakumar KR, Sudarshanakrishna KR, Chandramohan G, Ilaiyaraja N, Khanum F, Bawa AS. Effect of Aloe vera gel extract on antioxidant enzymes and azoxymethane-induced oxidative stress in rats. 2010.
Iji O, Oyagbemi A, Azeez O. Assessment of chronic administration of Aloe vera gel on haematology, plasma biochemistry, lipid profiles and erythrocyte osmotic resistance in Wistar rats. Nigerian J Physiol Sci. 2010;25(2):107-13.
Adesokan A, Oyewole O, Turay B. Kidney and liver function parameters in alloxan-induced diabetic rats treated with aloe barbadensis juice extract. Sierra leone J Biomed Res. 2009;1(1):33-7.
Tabrizi BA, Mohajeri D. Protective effect of Aloe Vera leaves extract on hepatic tissue injury in Streptozotocin-induced diabetic rats. Advan Biores. 2012;3(3).
Vahedi G, Taghavi M, Maleki AK, Habibian R. The effect of Aloe vera extract on humoral and cellular immune response in rabbit. African J Biotechnol. 2011;10(26):5225-8.
Bałan BJ, Niemcewicz M, Kocik J, Jung L, Skopińska-Różewska E, Skopiński P. Oral administration of Aloe vera gel, anti-microbial and anti-inflammatory herbal remedy, stimulates cell-mediated immunity and antibody production in a mouse model. Central-European J Immunol. 2014;39(2):125.
Channa AA, Soomro SA, Korejo RA, Qureshi TA, Shah IA, Kalhoro NA, et al. Effect of chronic administration of Aloe vera extract on plasma biochemistry in rabbits. Int J Med Med Sci. 2014;6(8):183-9.
Hegazy SK, El-Bedewy M, Yagi A. Antifibrotic effect of aloe vera in viral infection-induced hepatic periportal fibrosis. World J Gastroenterol: WJG. 2012;18(17):2026.
Noori S. An overview of oxidative stress and antioxidant defensive system. Open Access Sci Reports. 2012;1(8):1-9.
Coyle CH, Martinez LJ, Coleman MC, Spitz DR, Weintraub NL, Kader KN. Mechanisms of H2O2-induced oxidative stress in endothelial cells. Free Radical Biol Med. 2006;40(12):2206-13.
Davies KJ. Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement systems. IUBMB Life. 2000;50(4‐5):279-89.
Khudiar KK. Effect of 1% hydrogen peroxide (H2O2) in drinking water on some parameters inِ adult male rabbits. Iraqi J Biotech. 2010;9(2):202-10.
Lushchak VI. Environmentally induced oxidative stress in aquatic animals. Aquatic Toxicol. 2011;101(1):13-30.
Can A, Akev N, Ozsoy N, Bolkent S, Arda BP, Yanardag R, et al. Effect of Aloe vera leaf gel and pulp extracts on the liver in type-II diabetic rat models. Biol Pharmaceu Bulletin. 2004;27(5):694-8.
Amos AF, McCarty DJ, Zimmet P. The rising global burden of diabetes and its complications: estimates and projections to the year 2010. Diab Med. 1997;14(S5):S7-S85.
Falk E. Pathogenesis of atherosclerosis. J Am Coll Cardiol. 2006;47(8 Supplement):C7-C12.
Chandan B, Saxena A, Shukla S, Sharma N, Gupta D, Suri K, et al. Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity. J Ethnopharmacol. 2007;111(3):560-6.
Hamman J. Composition and applications of Aloe vera leaf gel. Molecules. 2008;13(8):1599-616.