Published: 2019-12-25

Comparison of haemoglobin assessment by HemoCue 301 and automated haematology analyser using flowcytometry among school going children: a one year study at a tertiary care hospital

Sarvepalli Vijaya Kumar, Krishnan Ramalingam


Background: Anaemia defined as reduction in the concentration in Haemoglobin is one of the key health indicators of health care system of the country. Accurate screening methods are required to estimate the levels of haemoglobin for diagnosing the cause of anaemia. Objectives of the study was to analyze and compare the results of haemoglobin concentrations estimated with automated haematology analyzer and point of care device HemoCue Hb301.

Methods: It is a prospective cross-sectional study was conducted for one year after ethical approval. Non fasting capillary and venous blood samples were collected from the selected cases of children and Haemoglobin concentrations were estimated by automated analyzer and HemoCue Hb301 system and the values were noted. Quality control checks were performed for both. Statistical analysis was done using IBM SPSS Version 24.0.

Results: Mean Hb% concentration was estimated in 108 children with 44 female and 64 males. The mean value of Automated hematology analyzer (11.965±1.012) was significantly higher when compared with the mean value of HemoCue Hb301 (11.697±1.312) (p=0.002). There was a significantly strong correlation between HemoCue Hb301and Automated hematology analyzer (r-value = 0.732, p <0.0001).

Conclusions: The HemoCue is useful in many different settings and remains a widely used method in field settings as it has several advantages and is relatively inexpensive compared with automated haematology analysers. Further studies are needed to better understand potential sources of error in the Hb assessment by HemoCue with the aim to better train phlebotomists and implement appropriate standardised procedures.


Anemia, Automated hematology analyzer, HemoCue Hb301, Hemoglobin

Full Text:



Bruno de Benoist, Erin McLean, Ines Egli and Mary Cogswell. Worldwide prevalence of anaemia 1993-2005. WHO Global Database on Anaemia Geneva, World Health Organization, 2008. Available at: Accessed on 14 November 2019.

McLean E, Cogswell M, Egli I, Wojdyla D, De Benoist B. Worldwide prevalence of anaemia, WHO vitamin and mineral nutrition information system, 1993-2005. Public Health Nutr. 2009 Apr;12(4):444-54.

Rippmann CE, Nett PC, Popovic D, Seifert B, Pasch T, Spahn DR. HemoCue®, an accurate bedside method of hemoglobin measurement?. J Clini Monitor. 1997 Nov 1;13(6):373-7.

Sari M, Pee SD, Martini E, Herman S, Bloem MW, Yip R. Estimating the prevalence of anaemia: a comparison of three methods. Bulletin World Health Org. 2001;79:506-11.

Tesfaye M, Yemane T, Adisu W, Asres Y, Gedefaw L. Anemia and iron deficiency among school adolescents: burden, severity, and determinant factors in southwest Ethiopia. Adolescent Health, Med Therap. 2015;6:189-96.

Hinnouho GM, Barffour MA, Wessells KR, Brown KH, Kounnavong S, Chanhthavong B, et al. Comparison of haemoglobin assessments by HemoCue and two automated haematology analysers in young Laotian children. J Clini Pathol. 2018 Jun 1;71(6):532-8.

Paiva AD, Rondó PH, Silva SS, Latorre MD. Comparison between the HemoCue® and an automated counter for measuring hemoglobin. Revista Saude Publica. 2004;38:585-7.

Chen PP, Short TG, Leung DH, Oh TE. A clinical evaluation of the Hemocue haemoglobinometer using capillary, venous and arterial samples. Anaesthesia Inten Care. 1992 Nov;20(4):497-500.

Rappaport AI, Barr SI, Green TJ, Karakochuk CD. Variation in haemoglobin measurement across different HemoCue devices and device operators in rural Cambodia. J Clini Pathol. 2017 Jul 1;70(7):615-8.

Rechner IJ, Twigg A, Davies AF, Imong S. Evaluation of the HemoCue compared with the Coulter STKS for measurement of neonatal haemoglobin. Arch Dis Childhood-Fetal Neonatal Edition. 2002 May 1;86(3):F188-9.

Adam I, Ahmed S, Mahmoud MH, Yassin MI. Comparison of HemoCue® hemoglobin-meter and automated hematology analyzer in measurement of hemoglobin levels in pregnant women at Khartoum hospital, Sudan. Diagnostic Pathol. 2012 Dec;7(1):30.