DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20200248

Serum levels of matrix metalloproteinase-2 in patients with oral submucous fibrosis and oral squamous cell carcinoma

Pooja Kherde, Suchitra Gosavi

Abstract


Background: Matrix metalloproteinase-2 (MMP-2), which is supposed to enable cancer cells cross the basement membrane and metastasize by selectively cleaving type IV collagen, is anticipated to be a good diagnostic and prognostic marker in oral squamous cell carcinoma (OSCC) and oral submucous fibrosis (OSMF). Thus, the study is aimed to estimate and compare the serum MMP-2 levels in patients with OSMF and OSCC.

Methods: The study was conducted on 88 subjects, divided into three groups; Group I (healthy subjects, n=28), Group II (patients with OSMF, n=30), and Group III (patients with OSCC, n=30). Serum levels of MMP-2 were estimated and compared among the groups and further with the clinical parameters within the groups.

Results: The mean serum MMP-2 levels in patients with OSMF (2.87±1.04 ng/mL) and with OSCC (11.55±2.16 ng/mL) were significantly higher than the healthy subjects (0.93±0.26 ng/mL) (p <0.0001, for both). Furthermore, the mean serum MMP-2 levels in OSMF subjects had a positive association with inter-incisal opening (IIO), however, there was no association with the degree of burning sensation. Likewise, in subjects with OSCC, levels of serum MMP-2 showed positive association with histopathological grades, however, significant association with the site of occurrence and primary tumor size was not found.

Conclusions: Elevated serum MMP-2 levels can be used as a screening tool in the early detection of OSMF and OSCC cases. Moreover, MMP-2 might be a good marker in evaluating the tumor grade in OSCC and the IIO in OSMF.


Keywords


Invasion and metastasis, Matrix metalloproteinase-2, Oral squamous cell carcinoma, Oral submucous fibrosis

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References


Shrestha A, Carnelio S. Evaluation of matrix metalloproteinases-2 (MMP-2) and tissue inhibitors of metalloproteinases-2 (TIMP-2) in oral submucous fibrosis and their correlation with disease severity. Kathmandu University Me J. 2015;11(4):274-81.

Satpute PS, Hazarey VK, Ganvir SM. Estimation of Matrix Metalloproteinases-2 Promoter Polymorphism as a Risk Factor for Oral Carcinogenesis in Indian Population. Open Access Scientific Reports. 2012;1(7):370.

Khanna JN, Andrade NN. Oral submucous fibrosis: a new concept in surgical management. Report of 100 cases. Int J Oral Maxillo Surg. 1995;24:433-9.

Markopoulos AK. Current aspects on oral squamous cell carcinoma. Open Dent J. 2012;6:126-30.

Warnakulsurya S. Global Epidemiology of oral and Pharyngeal Cancer. Oral Oncol. 2009;45:309-16.

Murti RR, Bhosale BB, Gupta PC, Daftary DK, Pindborg JJ, Mehta FS. Etiology of oral submucous fibrosis with special refrence to role of areca nut chewing. J Oral Pathol Med. 1995;24:145-52.

Pundir S, Saxena S, Aggrawal P. Oral submucous fibrosis a disease with malignant potential -Report of two Cases. J Clin Exp Dent. 2010;2:215-8.

Noël A, Gutiérrez-Fernández A, Sounni NE, Behrendt N, Maquoi E, Lund IK, et al. New and paradoxical roles of matrix metalloproteinases in the tumor microenvironment. Frontiers pharmacol. 2012;3:1-9.

Mishra G, Ranganathan K. Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical study. Ind J Dent Res. 2010;21(3):320-5.

Leppä S, Saarto T, Vehmanen L, Blomqvist C, Elomaa I. A high serum matrix metalloproteinase-2 level is associated with an adverse prognosis in node-positive breast carcinoma. Clini Cancer Res. 2004;10(3):1057-63.

Mishev G, Deliverska E, Hlushchuk R, Velinov N, Aebersold D, Weinstein F, et al. Prognostic value of matrix metalloproteinases in oral squamous cell carcinoma. Biotechnol Biotechnol Equip. 2014;28(6):1138-49.

Gohji K, Fujimoto N, Hara I, Fujii A, Gotoh A, Okada H, et al. Serum matrix metalloproteinase-2 and its density in men with prostate cancer as a new predictor of disease extension. Inter J Cancer. 1998;79(1):96-101.

Lotfi A, Mohammadi G, Saniee L, Mousaviagdas M, Chavoshi H, Tavassoli A. Serum level of matrix metalloproteinase-2 and-9 in patients with laryngeal squamous cell carcinoma and clinical significance. Asian Pac J Cancer Prev. 2015;16(15):6749-51.

Tsai CC, Sheih TY. Deficiency in collagen and fibronectine phagocytosis by human mucosa fibroblasts in vitro as possible mechanisms for oral submucous fibrosis. J Oral Pathol Med. 1999;28:59-63.

Nissinen L, Kähäri VM. Matrix metalloproteinases in inflammation. Biochimica et Biophysica Acta (BBA)-General Subjects. 2014;1840(8):257-80.

Patel BP, Shah SV, Shukla SN, Shah PM, Patel PS. Clinical significance of MMP‐2 and MMP‐9 in patients with oral cancer. Head Neck. 2007;29(6):564-72.

Ray JG, Ganguly M, Rao BS, Mukherjee S, Mahato B, Chaudhuri K. Clinico-epidemiological profile of oral potentially malignant and malignant conditions among areca nut, tobacco and alcohol users in Eastern India: A hospital based study. J Oral Maxillofac Pathol. 2013;17:45-50.

Shrestha A, Carnelio S. Evaluation of matrix metalloproteinases-2 (MMP-2) and tissue inhibitors of metalloproteinases-2 (TIMP-2) in oral submucous fibrosis and their correlation with disease severity. Kathmandu University Med J. 2015;11(4):274-81.

Ku KT, Wan L, Peng HC, Tsai MH, Tsai CH, Tsai FJ. Vascular endothelial growth factor gene-460 C/T polymorphism is a biomarker for oral cancer. Oral Oncol. 2005;41(5):497-502.

Kessenbrock K, Plaks V, Werb Z. Matrix metalloproteinases: regulators of the tumor microenvironment. Cell. 2010;141(1):52-67.