Minimum inhibitory concentration of squid ink Loligo sp. extract on growth of Staphylococcus aureus and Streptococcus mutans bacteria

Authors

  • Damajanty H. C. Pangemanan Department of Physiology, Faculty of Medicine, Sam Ratulangi University, Manado, North Sulawesi, Indonesia
  • Christy Mintjelungan Department of Public Dental Hygiene, Dentistry Study Program, Faculty of Medicine, Sam Ratulangi University, Manado, North Sulawesi, Indonesia

DOI:

https://doi.org/10.18203/2320-6012.ijrms20200529

Keywords:

Minimum inhibitory concentration, Squid ink Loligo sp., Staphylococcus aureus, Streptococcus mutans

Abstract

Background: Dental caries and periodontal disease can be caused by bacteria that attack hard and soft tissues in the oral cavity like Staphylococcus aureus and Streptococcus mutans bacteria One way to prevent infections caused by the bacteria are by using mouthwash. Currently, mouthwash that is widely used is mouthwash containing chlorhexidine, which has side effects in the form of tooth staining when used prolonged. The need for alternative medicines from nature that effectively cope with polymicrobial infections. One of the marine products that has pharmaceutical properties is squid, especially the ink it produces.

Methods: This research is a pure experimental study (true experimental design) with a randomized pretest-posttest control group design. Squid ink Loligo sp. extract, obtained by extracting squid ink Loligo sp., with maceration method using 96% ethanol solvent. This research uses serial dilution method with spectrophotometric testing method.

Results: After measuring the turbidity value in each treatment tube, it was found that at a concentration of 1.56% squid ink Loligo sp. extract began to inhibit the growth of Staphylococcus aureus and Streptococcus mutans bacteria.  This is proven because the absorbance value after and before incubation is fixed.

Conclusions: The minimum inhibitory concentration of squid ink Loligo sp. extract on the growth of Staphylococcus aureus and Streptococcus mutants at a concentration of 1.56%.

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Published

2020-02-26

How to Cite

Pangemanan, D. H. C., & Mintjelungan, C. (2020). Minimum inhibitory concentration of squid ink Loligo sp. extract on growth of Staphylococcus aureus and Streptococcus mutans bacteria. International Journal of Research in Medical Sciences, 8(3), 801–805. https://doi.org/10.18203/2320-6012.ijrms20200529

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Original Research Articles