DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20201352

Evaluation of hormonal profile and some stress biomarkers in infertile couples in Abuja, Nigeria

Simon P. Abriba, Humphrey B. Osadolor

Abstract


Background: Infertility is a growing gynaecological problem in couples of childbearing age having difficulties bearing children. Couples with infertility are known to present with high levels of stress and psychopathology A cross sectional case control study aimed at evaluating the hormonal profile, some stress biomarkers, sperm analysis in infertile couples was carried out to ascertain their contributions to infertility in couples of child bearing age.

Methods: Serum Luteinizing Hormone (LH), Follicle Stimulating Hormone (FSH), Prolactin, Progesterone, Estrogen (E2), Testosterone, Salivary Cortisol and Salivary Alpha Amylase were evaluated using both competitive and non-competitive Enzyme Linked Immunosorbent Assay (ELISA) techniques; while sperm cells analysis were evaluated using conventional methods, in 164 infertile couples (study) and 100 fertile couples (control) attending fertility clinic in General Hospitals in Abuja, Nigeria.

Results: The mean serum Prolactin and Salivary (S) Cortisol in the female were significantly higher (p<0.05) in the study group relative to the control group, while Salivary Alpha Amylase and LH show no significant difference (p>0.05) between the two groups. FSH, Progesterone and E2 in the female were significantly lower in the study group (p<0.05) compared with control group. Testosterone, FSH, Sperm cells count and Sperm activity (%) were significantly lower (p<0.05) in the male study group relative to the control group; while prolactin, S. cortisol and S. amylase were significantly higher (p<0.05) in the male study group relative to the control group. 

Conclusions: Abnormal hormones values and abnormal sperm quality and quantity are associated with elevated stress biomarkers in couples presenting with infertility. Strong positive correlations exist between hormones and stress biomarker in infertility conditions.


Keywords


Biomarkers of stress, Couples infertility, Hormonal profile, Sperm cells count

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References


Geidam AD, Yawe KD, Adebayo AE, Idrisa A. Hormonal profile of men investigated for infertility at the University of Maiduguri in northern Nigeria. Singapore Med J. 2008 Jul 1;49(7):538.

WHO. Fertility and Sterility. Human Reproduction. 2009;24(11):1-5.

Ben-Chioma AE, Tamuno-Emine DG. Evaluation of female fertility hormone profile in women with primary and secondary infertility. Inter J Sci Res. 2015;4(10):1583-5.

American College of Obstetricians and Gynecologists. ACOG (American College of Obstetricians and Gynecologists) educational bulletin. Adult manifestation of childhood sexual abuse, number 259, July 2000. Clinical management guidelines for obstetrician-gynecologists. Int J Gynaecol Obstet. 2001 Sep;74:311-20.

Ferin M, Jewelewicz R, Warren M. The menstrual cycle: physiology, reproductive disorders, and infertility. Oxford University Press, USA; 1993.

Oddens BJ, den Tonkelaar I, Nieuwenhuyse H. Psychosocial experiences in women facing fertility problems a comparative survey. Human Reprod. 1999 Jan 1;14(1):255-61.

Brkovich AM, Fisher WA. Psychological distress and infertility: forty years of research. J Psych Obstetr Gynecol. 1998 Jan 1;19(4):218-28.

Inhorn M, Van Balen F, editors. Infertility around the globe: New thinking on childlessness, gender, and reproductive technologies. Univ of California Press; 2002 May 30.

Henning K, Strauss B. Psychological and Psychosomatic Aspects of Involuntary Childlessness. State of Research at the End of the 1990s. Ash/and Off: Hogrefe and Huber. 2002;340-51.

Pook M, Tuschen‐Caffier B, Krause W. Is infertility a risk factor for impaired male fertility?. Human Reprod. 2004 Apr 1;19(4):954-9.

Okonofua F. Infertility and Women's Reproductive Health in Africa/Infertilité et Santé Reproductive des Femmes en Afrique. Afri J Reprod Health/La Revue Afric de La Santé Reprod. 1999 May 1;3(1):7-12.

Aghanwa HS, Dare FO, Ogunniyi SO. Sociodemographic factors in mental disorders associated with infertility in Nigeria. J Psych Res. 1999 Feb 1;46(2):117-23.

Lynch CD, Sundaram R, Maisog JM, Sweeney AM, Buck Louis GM. Preconception stress increases the risk of infertility: results from a couple-based prospective cohort study the LIFE study. Human Reprod. 2014 May 1;29(5):1067-75.

Digban KA, Enitan SS, Otuneme OG, Adama S. Hormonal Profile of women of Reproductive age investigated for Infertility in Bida Metropolis, Niger State, Nigeria. Scholars J Appl Med Sci. 2017; 5(5A):1750-7.

Willocks J, Neilson JP. Student notes. Obstetrics and Gynecology. 4th ed. Edinburgh: Churchill Livingstone; 1991:271-280.

Guidice LC, Kao LC. Endometriosis and Infertility. Lancet. 2004;364(9447):789-99.

Isah IA. Pattern of Reproductive Hormones in Women with Infertility in Zaria. Northen Nigeria. 2009:1-187.

Cates W, Farley TM, Rowe PJ. Worldwide patterns of infertility: is Africa different?. Lancet. 1985 Sep 14;326(8455):596-8.

Morgan SP, Rackin H. The correspondence between fertility intentions and behavior in the United States. Popul Develop Rev. 2010 Mar;36(1):91-118.

Evers JL. Female subfertility. Lancet. 2002 Jul 13;360(9327):151-9.

Prelevic GM, Jacobs HS. Menopause and Post- menopause. Baillie Clin Endocrinol Metab. 1997;11(2):311-40.

Gurunath S, Pandian Z, Anderson RA, Bhattacharya S. Defining infertility a systematic review of prevalence studies. Human Repro Update. 2011 Sep 1;17(5):575-88.

Emokpae MA, Uadia PO, Omale-Itodo A, Orok TN. Male infertility and endocrinopathies in Kano, Northwestern Nigeria. Annal Afri Med. 2007 Jun 1;6(2):64.

Lieberman HR, Farina EK, Caldwell J, Williams KW, Thompson LA, Niro PJ, et al. Cognitive function, stress hormones, heart rate and nutritional status during simulated captivity in military survival training. Physiol Beha. 2016 Oct 15;165:86-97.

Tellam DJ, Mohammad YN, Lovejoy DA. Molecular integration of hypothalamo-pituitary-adrenal axis-related neurohormones on the GnRH neuron. Biochem Cell Biol. 2000 Apr 2;78(3):205-16.

Gollenberg AL, Liu F, Brazil C, Drobnis EZ, Guzick D, Overstreet JW, et al. Semen quality in fertile men in relation to psychosocial stress. Fertil Steril. 2010 Mar 1;93(4):1104-11.

Wdowiak A, Bien A, Iwanowicz-Palus G, Makara-Studzińska M, Bojar I. Impact of emotional disorders on semen quality in men treated for infertility. Neuroendocrinol Letters. 2017 Jan 1;38(1).

Morgan CA, Southwick S, Hazlett G, Rasmusson A, Hoyt G, Zimolo Z, et al. Relationships among plasma dehydroepiandrosterone sulfate and cortisollevels, symptoms of dissociation, and objective performance in humans exposedto acute stress. Archiv General Psych. 2004 Aug 1;61(8):819-25.

Eskiocak S, Gozen AS, Yapar SB, Tavas F, Kilic AS, Eskiocak M. Glutathione and free sulphydryl content of seminal plasma in healthy medical students during and after exam stress. Human Reprod. 2005 Sep 1;20(9):2595-600.

Zorn B, Auger J, Velikonja V, Kolbezen M, Meden‐Vrtovec H. Psychological factors in male partners of infertile couples: relationship with semen quality and early miscarriage. Inter J Androl. 2008 Dec;31(6):557-64.

Bhongade MB, Prasad S, Jiloha RC, Ray PC, Mohapatra S, Koner BC. Effect of psychological stress on fertility hormones and seminal quality in male partners of infertile couples. Andrologia. 2015 Apr;47(3):336-42.

Lovely LP, Meyer WR, Ekstrom RD, Golden RN. Effect of stress on pregnancy outcome among women undergoing assisted reproduction procedures. Southern Med J. 2003 Jun 1;96(6):548-52.

Nepomnaschy PA, Welch KB, McConnell DS, Low BS, Strassmann BI, England BG. Cortisol levels and very early pregnancy loss in humans. Proceed Nati Acad Sci. 2006 Mar 7;103(10):3938-42.