Isolation, identification and antibiotic sensitivity pattern of Escherichia coli isolated from various clinical sample in a tertiary care hospital, Jaipur, Rajasthan, India

Bitopan Das, Mrinmoy Sarma, Rohit Kumar, Palashi Borah, Sushama Sinha, Prasanna Gupta, Pushpa Durlabhji


Background: Escherichia coli is one of the most frequent causes of many bacterial infections, including Urinary Tract Infections (UTI), blood stream infections, otitis media, pneumonia, meningitis, traveler’s diarrhoea, enteric infections and systemic infections. This study was done with the aim to surveying antibiotic sensitivity pattern of isolated Escherichia coli in both sex attended in NIMS Hospital, Jaipur under the taken time period.

Methods: In this cross-sectional study, 62 Escherichia coli were isolated from various clinical specimens of the patients attending both OPD and IPD. The strains were selected using the laboratory standard methods and culture-specific. The antibiotic susceptibility testing was performed using Kirby-Bauer disk diffusion method.

Results: Out of total 62 isolates of Escherichia coli 26(41.93%) isolates were from male while 36(58.064%) from female patients. Maximum sensitivity were shown by Polymyxin B and Colistin i.c 100% followed by Nitrofuratonin 82.5% followed by Meropenem 79.03%, Aztreonam 72.58%, Piperacillin/ Tazobactam and Ciprofloxacin 61.30%, each Amikacin 56.45%, Imipenem 54.83%, Ofloxacin 45.16%, Cefepime 43.54%, Ceftazidime 38.71%, Gentamycin and Ceftriaxone 37.09% each, Cefotaxime 30.64%, Norfloxacin 27.5%. Maximum resistance shown against Norfloxacin 72.5%, followed by Gentamycin and Ceftriaxone 62.90%, Ceftazidime 61.30%.

Conclusions: Escherichia coli infected more in urinary tract infection as compare to other sample in human, and it is common in female than male. Regular monitoring of antimicrobial susceptibility for E.coli is recommended to improve treatment. A changing trend in antibiotic sensitivity profile of the isolates need to be monitored as there is limited availability of newer drugs and the emergence of resistant bacteria far exceeds the rate of new drug development.


Antibiotic resistant, Escherichia coli, Sensitivity pattern of E.coli, Urinary tract infection

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Kashef N, Djavid GE, Shahbazi S. Antimicrobial susceptibility patterns of community-acquired uropathogens in Tehran, Iran. J Infect Develop Countr. 2010 Jan 20;4(04):202-6.

Serefhanoglu K, Turan H, Timurkaynak FE, Arslan H. Bloodstream infections caused by ESBL-producing E. coli and K. pneumoniae: risk factors for multidrug-resistance. Brazili J Infectious Dis. 2009 Dec;13(6):403-7.

Alsaimary IE, Alabbasi AM, Najim JM. Antibiotics susceptibility of bacterial pathogens associated with otitis media. African J Bacteriol Res. 2010 Sep;2(4):41-50.

Alberto ruiz L, Zalacain R, Gomez A, Camino J, Jaca C, Manuel núñez J. Escherichia coli: an unknown and infrequent cause of community acquired pneumonia. Scandinavian J Infectious Diseases. 2008 Jan 1;40(5):424-7.

Moissenet D, Salauze B, Clermont O, Bingen E, Arlet G, Denamur E, et al. Meningitis caused by Escherichia coli producing TEM-52 extended-spectrum β-lactamase within an extensive outbreak in a neonatal ward: epidemiological investigation and characterization of the strain. J Clini Microbiol. 2010 Jul 1;48(7):2459-63.

Van der Plas H. Microbiological evaluation and antimicrobial treatment of complicated intra-abdominal infections. South Afric J Epidemiol Infect. 2012 Jan 1;27(2):53-7.

Kaper JB, Nataro JB, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol. 2014;2:123-40.

Kim SA, Kim DW, Dong BQ, Kim JS, Anh DD, Kilgore PE. An expanded age range for meningococcal meningitis: molecular diagnostic evidence from population-based surveillance in Asia. BMC Infect Dis. 2012 Dec;12(1):310.

Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, et al. Diversit Human Intest Microb Flora. Science. 2005 Jun 10;308(5728):1635-8.

Pitout JD. Extraintestinal pathogenic Escherichia coli: an update on antimicrobial resistance, laboratory diagnosis and treatment. Expert Rev Anti-Infective Ther. 2012 Oct 1;10(10):1165-76.

Faraji R, Sabzi F, Center IA. Antimicrobial susceptibility of Escherichia coli isolated from patients with urinary tract infection referred to Imam Ali Hospital Kermanshah, Iran (2011). Life Sci J. 2012;9(3):1679-82.

Alp E, Güven M, Yıldız O, Aygen B, Voss A, Doganay M. Incidence, risk factors and mortality of nosocomial pneumonia in intensive care units: a prospective study. Ann Clini Microbiol Antimicrob. 2004 Dec;3(1):17.

Orrett FA, Shurlandl SM. Prevalence of Resistance to Antimicrobials of Escherichia coli Isolates fTrom Clinical Sources at a Private Hospital in Trinidad. JPN. J Infect Dis. 2001;54:64-8.

Sowmya N, Savitha S, Mallure S, Mohanakrishnan K, Sumathi G, Arumugam P. A two year study of spectrum of bacterial isolates from wound infections by aerobic culture and their antibiotic pattern in a tertiary care center. Int J Curr Microbiol App Sci. 2014;3(8):292-5.

Rameshkannan S, Nileshraj G, Rameshprabu S, Mangaiarkkarasi A, MeherAli R. Pattern of pathogens and their sensitivity isolated from pus culture reports in a tertiary care hospital, Puducherry. Ind J Basic App Med Res. 2014;4(1):243-8.

Jafari-Sales A, Rasi-Bonab F. Detection of the antibiotic resistance pattern in Escherichia coli isolated from urinary tract infections in Tabriz City. J Mol Microbiol. 2017;1(1):1-3.

Akter T, Hossain MJ, Khan MS, Sultana H, Fatema K, Al Sanjee S, et al. Isolation, identification and antimicrobial susceptibility pattern analysis of Escherichia coli isolated from clinical samples of Bangladesh. Asian J Biomedi Pharmaceut Sci. 2016 Jan 1;6(54):13.

Manu C, Anurag P. Prospective Study for Antimicrobial Susceptibility of Escherichia coli Isolated from Various Clinical Specimens in India. J Microb Biochem Technol. 2012;4:157-60.

Sabir S, Anjum AA, Ijaz T, Ali MA. Isolation and antibiotic susceptibility of E. coli from urinary tract infections in a tertiary care hospital. Pak J Medi Scie. 2014 Mar;30(2):389.