DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20202251

Bacterial pathogens in wound infection and their antimicrobial susceptibility pattern in a medical college hospital, in Dhaka, Bangladesh

Sadia Afroz, Dipika Sarkar, Khadeza Khatun, Tarek Mahbub Khan, Shikha Paul

Abstract


Background: Wound infection is a major health problem that results in prolong hospital stay, increased treatment cost and are responsible for significant mortality and morbidity worldwide. The aim of the present study was to isolate and identify the bacterial pathogens causing wound infection and to determine their antimicrobial susceptibility profile.

Methods: This cross sectional study was conducted at the Department of Microbiology, Sir Salimullah Medical College, Dhaka from January 2016 to December 2016. Wound swab samples were collected and inoculated into appropriate media. The bacterial pathogens were identified by using standard microbiological methods. Antimicrobial susceptibility test were performed using disc diffusion technique following Kirby-Bauer method.

Results: Out of 239 wound swab samples analyzed 173 (72.4%) were culture positive. Majority (35.3%) of culture positive cases were in age group 16-30 years and 60.1% were male. Staphylococcus aureus (36.9%) was the predominant isolate, followed by Escherichia coli (35.8%), Pseudomonas spp. (17.3%) and Proteus spp. (5.8%). Bacterial isolates were highly resistant Amoxicillin (89-100%), Cephalosporin (60-100%), Ciprofloxacin (53-71%), while they were least resistant to Imipenem (0-14%) and Amikacin (17-30%).  

Conclusions: In the present study most of the isolates showed high rate of resistance to commonly used antibiotics. Therefore regular monitoring and rational use of antibiotic should be practiced.


Keywords


Antimicrobial resistance, Bacterial pathogen, Wound infection

Full Text:

PDF

References


Mordi MR, Momoh MI. Incidence of Proteus Species in Wound infections and their Sensitivity Pattern in the University of Benin Teaching Hospital. Afr J Biotechnol 2009;8(5):725-30.

Ogba OM, Olorode OA, Adie GP. Bacterial pathogens associated with wound infections in Calabar, Nigeria. J Med. 2014;13(1):26-33.

Razavi SM, Ibrahimpur M, Kashani AS, Jafarian A. Abdominal Surgical Site Infections: Incidence and Risk factors at an Iranian Teaching Hospital. BMC Surgery. 2005;5:2-6.

Weledji E. Bacterial organisms in acute wounds, implications on surgical wound management. J Med Sci. 2012;3(10):610-15.

Sultana S, Mawla N, Kawser S, Akhter N, Ali MK. Current Microbial Isolates from Wound Swab and Their Susceptibility Pattern in a Private Medical College Hospital in Dhaka city. Delta Med Col J. 2015;3(1):25-30.

Rahman M, Jobayer M, Akter N, Ahamed F, Shamsuzzaman SM, Mamun KZ. Rapid detection of Pseudomonad at species level by multiplex PCR in surgical units and ICU of Dhaka Medical College Hospital. Bangladesh J Med Microbiol. 2016;10(2):22-6.

Sule A, Thanni L, Sule-Odu O, Olusanya O. Bacterial pathogens assoaciated with infected wounds in Ogun state University Teaching Hospital, Sagamu, Nigeria. Afr J Clin Exp Microbiol. 2002;3(1):13-6.

Obritsch MD, Fish DN, MacLaren R, Jung R. Natioal surveillance of antimicrobial resitance in Pseudomonas aeruginosa isolates obtained from intensive care unit patients from 1993 to 2002. Antimicrob Agents Chemother. 2004;48(12):4606-10.

Anguzu JR, Olila D. Drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a regional referral hospital in Uganda. Afr Health Sci. 2007;7(3):148-54.

Taye M, Animut GA, Seid J. Antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia. Asian Pac J Trop Biomed. 2011;1(5);370-5.

Mohammed A, Seid ME, Gebrecherkos T, Tiruneh M, Moges F. Bacterial Isolates and Their Antimicrobial Susceptibility Patterns of Wound Infections among Inpatients and Outpatients Attending the University of Gondar Referral Hospital, Northwest Ethiopia. Int J Microbiol. 2017;10:1-10.

Sani RA, Garba SA, Oyewole OA. Antibiotic resistance profile of gram negative bacteria isolated from surgical wounds in Minna, Bida, Kontagora and Suleja Areas of Niger State. Am J Med Sci. 2012;2(1):20-4.

Shittu AO, Kolawole DO, Oyedepo EAR. Wound infections in two health institutions in Ife-Ife, Nigeria: Result of A Cohort Study. Ostomy/Wound Manage. 2012;49(5):52-7.

Egbe C, Omoregie R, Igbarumah I, Onemu S. Microbiology of wound infections among patients of a Tertiary Hospital in Benin City, Nigeria. J Res Health Sci. 2011;11(2):109-13.

Collee JG, Marr W. Specimen collection, culture containers and media. In: Collee JG, Duguid JP, Fraser AG, Marmion BP eds. Mackie & McCartney Practical Medical Microbiology, Vol. 2, 14th ed. Churchill Livingstone, New York; 1996: 85-111.

Wayne P. CLSI performance standard of antimicrobial susceptibility testing: twenty-fourth international supplement. CLSI Document M100-S24, Clini Lab Std Instit. 2014;34(1):50-106.

Mayhall CG. Nosocomial burn wound infection. In: Willium and Wilkins Co eds. Hospital epidemiology and infection control. Baltimore, USA; 1996: 225-236.

Sader HS, Jones RN, Silva JB. Skin and soft tissue infection in Latin American Medical centers: four years assessment of pathogen frequency and antimicrobial susceptibility patterns. Diagn Microbiol Infect Dis. 2002;44:281-88.

Azene MK, Beyene BA. Bacteriology and antibiogram of pathogens from wound infections at Dessie Laboratory, North East Ethiopia. Tanzania J Health Res. 2011;13(4):1-10.

Abraham Y, Wamisho BL. Microbial susceptibility of bacteria isolated from fracture wounds presenting to the err of black-lion hospital. Addis Ababa University, Ethiopia. Afr J Microbiol Res, 2009;3(1):939-51.

Khanam RA, Islam MR, Sharif A, Parveen R, Sharmin I, Yusuf MA. Bacteriological Profiles of Pus with Antimicrobial Sensitivity Pattern at a Teaching Hospital in Dhaka City. Bangladesh J Infect Dis. 2018;5(1):10-4.

Mama M, Abdissa A, Sewunet T. Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University Specialized Hospital, South-West Ethiopia. Ann Clin Microbiol Antimicrob. 2014;13(14):1-10.

Pondei K, Fente BG, Oladapo O. Current microbial isolates from wound swabs, their culture and sensitivity pattern at the Niger Delta University Teaching Hospital, Okolobiri, Nigeria. Trop Med Health. 2013;41(2);49-53.

Basu S, Ramchuran PT, Balin Sing T, Gulati AK, Shukla VK. A prospective, descriptive study to identify the microbiological profile of chronic wounds in outpatients. Ostomy Wound Manage. 2009;55(1):14-20.