Published: 2020-07-24

Management of a case of symblepharon by freshly prepared amniotic membrane transplantation: a clinical case report

Mamoni Baruah, Rajiv Kr. Das, Bikram Dam, Sunanda Nandi


Amniotic membrane transplantation has been in common therapeutic use for a variety of ocular disorders. Authors present a case of a 50-year-old man who was diagnosed to be a case of total symblepharon following an episode of membranous conjunctivitis who was treated with freshly prepared Amniotic membrane transplantation with satisfactory cosmetic result.


Amniotic membrane transplantation, Conjunctivitis, Symblepharon

Full Text:



Akle CA, Adinolfi M, Welsh KI, Leibowitz S, McColl I. Immunogenicity of human amniotic epithelial cells after transplantation into volunteers. Lancet 1981;318:1003-5.

Dekaris I, Gabrić N. Preparation and preservation of amniotic membrane. Dev Ophthalmol 2009;43:97-104.

Dua HS, Azuara-Blanco A. Amniotic membrane transplantation. Br J Ophthalmol. 1999;83:748-52.

Fotopoulou C, Sehouli J, Gehrmann N, Schoenborn I, Lichtenegger W. Functional and anatomic results of amnion vaginoplasty in young women with Mayer–Rokitansky–Kuster–Hauser syndrome. Fertil Steril. 2010;94:317-23.

Grueterich M, Espana EM, Tseng SCG. Ex vivo expansion of limbal epithelial stem cells: amniotic membrane serving as a stem cell niche. Surv Ophthalmol. 2003;48:631-46.

Guo M, Grinnell F. Basement membrane and human epidermal differentiation in vitro. J Investig Dermatol. 1989;93:372-8.

Hao Y, Ma DH-K, Hwang DG, Kim W-S, Zhang F. Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane. Cornea. 2000;19:348-52.

Hasegawa M, Fujisawa H, Hayashi Y, Yamashita J. Autologous amnion graft for repair of myelomeningocele: technical note and clinical implication. J Clin Neurosci. 2004;11:408-11.

Hennerbichler S, Reichl B, Pleiner D, Gabriel C, Eibl J, Redl H. The influence of various storage conditions on cell viability in amniotic membrane. Cell Tissue Bank. 2007;8:1-8.

Hori J, Wang M, Kamiya K, Takahashi H, Sakuragawa N. Immunological characteristics of amniotic epithelium. Cornea. 2006;25:S53-8.

Kjaergaard N, Hein M, Hyttel L, Helmig RB, Schønheyder HC, Uldbjerg N, et al. Antibacterial properties of human amnion and chorion in vitro. Eur J Obstet Gynecol Reprod Biol 2001;94:224-9.

Kjaergaard N, Helmig RB, Schønheyder HC, Uldbjerg N, Hansen ES, Madsen H. Chorioamniotic membranes constitute a competent barrier to group B streptococcus in vitro. Eur J Obstet Gynecol Reprod Biol. 1999;83:165-9.

Kruse FE, Rohrschneider K, Völcker HE. Multilayer amniotic membrane transplantation for reconstruction of deep corneal ulcers. Ophthalmology. 1999;106:1504-11.

Kurpakus MA, Stock EL, Jones JCR. The role of the basement membrane in differential expression of keratin proteins in epithelial cells. Dev biol. 1992;150:243-55.

Lee S-B, Li D-Q, Tan DTH, Meller D, Tseng SCG. Suppression of TGF-β signaling in both normal conjunctival fibroblasts and pterygial body fibroblasts by amniotic membrane. Curr Eye Res. 2000;20:325-34.

Lee S-H, Tseng SCG. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol. 1997;123:303-12.

Madhavan HN, Priya K, Malathi J, Joseph PR Preparation of amniotic membrane for ocular surface reconstruction. Indian J ophthalmol. 2002;50:227-31.