DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20204213

Antiulcerogenic effect of Capparis cartillaginea decne on indomethacin induced gastric ulcer in wistar rats

Nancy W. Mugo, Christine Wangia, Gideon Kikuvi, Samuel Ngugi

Abstract


Background: Peptic ulcer disease is a non-malignant, mucosal lesion of the stomach or duodenum. The mucosal defect reaches the muscularis mucosa and sometimes, beyond causing life threatening complications, including haemorrhage, perforations, gastrointestinal obstruction and malignancy.

Methods: The animals were pre-treated with omeprazole 20 mg/kg and 300 mg/kg of Capparis cartillaginea decne orally for 14 days. On the 15th day, ulcers were induced using indomethacin 30 mg/kg and 4 hours post ulcer induction, they were sacrificed. Ulcer index, pH, total acidity and volume were determined.

Results: Extensive lesions were seen in indomethacin ulcerated rats with mean ulcer score of (1.260±0.18). In comparison, there were minimal areas of erosion on animals pre-treated with omeprazole (0.14±0.025) and plant extracts (0.280±0.097). Indomethacin-induced ulcer treated animals showed the highest volume of gastric juice output (3.14±0.21 ml), whereas the animals pre-treated with omeprazole had lower gastric juice output (2.20±0.2 9ml). This was comparable to animals pre-treated with the plant extract (1.80±0.13 ml). The pH was high in animals pre-treated with omeprazole (5.02±0.53). This was also seen in animals pre-treated with the extract (4.82±0.31). This was in comparison to the low pH seen in indomethacin ulcerated animals (2.20±0.16). Indomethacin-induced ulcer treated animals showed high levels of total acidity (88.64±1.71 mEq/L). Whereas the animals pre-treated with omeprazole had lower total acidity (55.26±3.77 mEq/L), which was also mirrored in animals pre-treated with the plant extracts (61.44±2.42 mEq/L).

Conclusions: The extracts of Capparis cartillaginea decne showed anti-ulcer effect on indomethacin induced ulcers in Wistar rats.


Keywords


Indomethacin, Omeprazole, Caparris cartillaginea decne, Peptic ulcers

Full Text:

PDF

References


Entitlement eligibility guidelines peptic ulcer disease. Available at: https://gi.org/topics/peptic-ulcer-disease/. Accessed on 25 May 2020.

Abdelwahab M, Abourehab S. Peptic Ulcer : Mini Review. Available at: https://www.avidscience.com/ wp-content/uploads/2017/09/peptic-ulcer-mini-review.pdf. Accessed on 25 May 2020.

Kumar A, Ashwlayan V, Verma M. Diagnostic approach & pharmacological treatment regimen of Peptic Ulcer Disease. Phar Pharm Res Open Acc J. 2019;1(1):1-12.

Laine L, Takeuchi K, Tarnawski A. Gastric mucosal defense and cytoprotection: bench to bedside. Gastroenterology. 2008;135(1):41-60.

Whittle BJ. Gastrointestinal effects of nonsteroidal anti-inflammatory drugs. Fundam Clin Pharmacol. 2003;17(3):301-13.

Jiménez MD, Martín MJ, Alarcón de la Lastra C, Bruseghini L, Esteras A, Herrerias JM, et al. Role of L-arginine in ibuprofen-induced oxidative stress and neutrophil infiltration in gastric mucosa. Free Radic Res. 2004;38(9):903-11.

Wangia CO, Orwa JA, Muregi FW, Kareru PG, Cheruiyot K, Kibet J. Comparative anti-oxidant activity of aqueous and organic extracts from kenyan ruellia lineari- bracteolata and ruellia bignoniiflora. European Journal of medicinal plants. 2017;17(1):1-7.

Iqbal Z, Lateef M, Jabbar A, Akhtar MS, Khan MN. Anthelmintic activity of vemonia anthelmintica seeds against trichostrongylid nematodes of sheep. Pharmaceutical Biology. 2006;44(8):563-7.

Sayanti RC, Susri C, Subrata KB. Healing properties of some Indian medicinal plants against indomethacin-induced gastric ulceration of rats. J Clin Biochem Nutr. 2007;41(2):106-14.

Adinortey MB, Ansah C, Galyuon I, Nyarko A. In vivo models used for evaluation of potential antigastroduodenal ulcer agents. Ulcers. 2013; 2013:1-12.

Wang GZ, Huang GP, Yin GL, Zhou G, Guo CJ, Xie CG, et al. Aspirin can elicit the recurrence of gastric ulcer induced with acetic acid in rats. Cellular Physiology and Biochemistry. International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology. 2007;20(1-4):205-12.

Fornai M, Colucci R, Antonioli L, Awwad O, Ugolini C, Tuc-cori M, et al. Effects of esomeprazole on healing of nonsteroidalanti-inflammatory drug (NSAID)-induced gastric ulcers in the presence of a continued NSAID treatment: characterization of molecular mechanisms. Pharmacol Res. 2011;63(1): 59-67.

Biplab KY, Sudhir R, Kshama KB, Sandip C. Black tea and the aflavins assist healing of indomethacin-induced gastric ulceration in mice by antioxidative action. Evid Based Complem Alt Med. 2011;11:11-22.

Lüllmann H, Mohr K, Ziegler A, Bieger D. Colour atlas of pharmacology. 2nd ed. New York: Thieme Stuttgart; 2000:166.

Bech PL, Xavier R, Lu N, Nanda NN, Dinauer M, Podolsky DK, et al. Mechanisms of NSAID-induced gastrointestinal injury defined using mutant mice. Gastroenterology. 2000;119(3):699-705.

Muhammed AVK, Thamotharan G, Sengottuvelu S, Haja-Sherief S, Sivakumar T. Evaluation of antiulcer activity of Ficus pumila L. leaf extract in albino rats. Glob J Res Med Plants Indig Med. 2012;1(8):340-51.

Allen A, Flemström G. Gastro duodenal mucus bicarbonate barrier: Protection against acid and pepsin. American journal of Cell physiology. 2004;288:C1-19.

Sabiu S, Garuba T, Sunmonu T, Ajani E, Sulyman A, Nurain I, Abdulazeez B. Indomethacin-induced gastric ulceration in rats: protective roles of spondias mombin and ficus exasperata. Toxicology Reports. 2015;2:261-7.