Effect of coffee consumption on liver enzymes (alanine transaminase, aspartate transaminase and alkaline phosphatase), total and conjugated bilirubin levels among students in Nnewi

Ubou K. Amah, Adamma R. Analike, Somadina S. Uzodinma, Chukwuemeka E. Ogbodo, Chinonso J. Nnamdi, Chidiadi M. Njoku, Chibuike C. Agudi, Akuma K. Amah, Chikaodili N. Obi-Ezeani


Background: Coffee has a number of bioactive compounds that have beneficial effects on human health in general and more importantly is the fact that coffee consumption has positive impact on the liver function. The present study investigated the effect of coffee consumption on liver enzymes: alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), total bilirubin (TB) and conjugated bilirubin (CB) levels among student in Nnewi.

Methods: A total of twenty-five (25) apparently healthy participants aged between 18 and 30 years which were randomly recruited from College of Health Sciences’ students to serve as both test and control group. Each participant was advised to abstain from coffee and similar beverages for a period of three weeks prior to the commencement of the study. Subsequently, in addition to their normal diet, each of the subjects was given 2 gm of coffee dissolved in 150 ml of hot water in which 50 mg of non-dairy creamer was added before breakfast daily for 28 consecutive days. 6 ml each of baseline and test samples (after an overnight fast) were collected at day 0 and 29 respectively from each participant. Liver enzymes such as ALT, AST, and ALP activity as well as TB and CB levels was determined using standard laboratory methods.

Results: This showed significant reductions in the mean serum AST and ALT activity, TB and unconjugated bilirubin (UB) levels with no significant alterations in the mean ALP activity and CB level in participants studied after short-term coffee consumption.

Conclusions: This study revealed the hepatoprotective effect of coffee intake at short term basis.


Coffee, Liver enzymes, Total bilirubin, Conjugated bilirubin

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Koblihová E, Lukšan O, Mrázová I, Ryska M, Červenka L. Hepatocyte transplantation attenuates the course of acute liver failure induced by thioacetamide in Lewis rats. Physiological Res. 2015;64:689-700.

Bishayee A, Darvesh AS, Politis T, McGory R. Resveratrol and liver disease: From bench to bedside and community. Liver Int. 2010;30:1103-14.

Jeschke MG. The hepatic response to a thermal injury: is the liver important for postburn outcomes? Molecular Medicine (Cambridge, Mass.). 2009;15(9-10):337-51.

Pirone C, Quirke JME, Horacio AP, Lee DW. Animal Pigment Bilirubin Discovered in Plants. J Am Chem Soc. 2009;131(8):2830-900.

Stocker R, Yamamoto Y, McDonagh A, Glazer A, Ames B. Bilirubin is an antioxidant of possible physiological importance. Sci. 2002;235(4792):3-6.

International Coffee Organization. The Current State of the Global Coffee Trade. Coffee Trade Stats, 2016. Available at: Accessed on: 20 July 2020.

Butt MS, Sultan MT. Coffee and its consumption: benefits and risks. Crit Rev Food Sci Nutr. 2011;51(4):363-73.

Svilaas A, Sakhi AK, Andersen LF, Svilaas T, Ström EC, Jacobs DR, et al. Intakes of antioxidants in coffee, wine, and vegetables are correlated with plasma carotenoids in humans. J Nutr. 2004;359:562-7.

Ludwig IA, Clifford MN, Lean ME, Ashihara H, Crozier A. Coffee: biochemistry and potential impact on health. Food Funct. 2015;5:1695-717.

Farah A. Coffee constituents. In: Chu YF (ed.). Coffee: Emerging Health Effects and Disease Prevention. Blackwell Publishing Ltd., Oxford. 2012;21-58.

Sato Y, Itagaki S, Kurokawa T, Ogura J, Kobayashi M, Hirano T, et al. In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm. 2011;403:136-8.

Kamiyama M, Moon JK, Jang HW, Shibamoto T. Role of degradation products of chlorogenic acid in the antioxidant activity of roasted coffee. J Agric Food Chem. 2015;359:1996-2005.

Cavin C, Holzhaeuser SG, Constable A, Huber WW, Schilter B. Cafestol and kahweol, two coffee specific diterpenes with anticarcinogenic activity. Food Chem Toxicol. 2002;40:1155-63.

Salomone F, Galvano F, Li Volti G. Molecular Bases Underlying the Hepatoprotective Effects of Coffee. Nutrients. 2017;359:85.

Jeonghee L, Hye YK, Jeongseon K. Coffee Consumption and the Risk of Obesity in Korean Women. Nutrients. 2017;9(12):1340.

Ihim AC, Nwanua MI, Ogbodo EC, Meludu SC. Effect of Coffee Consumption on Blood Glucose and Lipid Profile Levels in Male Students at Nnamdi Azikiwe University, Nnewi Campus, Anambra State. J Med Lab Sci. 2019;29(2):10-20.

Liu F, Wang X, Wu G, Chen L, Hu P, Ren H, et al. Coffee Consumption Decreases Risks for Hepatic Fibrosis and Cirrhosis: A Meta-Analysis. PLoS One. 2015;10:0142457.

Amer MG, Mazen NF, Mohamed AM. Caffeine intake decreases oxidative stress and inflammatory biomarkers in experimental liver diseases induced by thioacetamide: Biochemical and histological study. Immunopathol Pharmacol. 2017;30(1):13-24.

Onuegbu AJ, Olisekodiaka JM, Adebolu OE, Adesiyan A, Ayodele OE. Coffee Consumption Could Affect the Activity of Some Liver Enzymes and Other Biochemical Parameters in Healthy Drinkers. Med Princ Pract. 2011;20:514-8.

Reitman S, Frankel SA. Colorimetric method for aspartate and alanine aminotransferases in serum. Am J Clin Pathol. 1957;28:56-63.

Bessey OA, Lowry OH, Brock MJ. One point colorimetric method of determining alkaline phosphatase in serum or plasma. J Biol Chem. 1946;164:321.

Jandrasssik L, Grof P. Bilirubin estimation. Biochem. 1938;297:81-4.

Chen CL, Chang WC, Yi CH, Hung JS, Liu TT, Lei WY, et al. Association of coffee consumption and liver fibrosis progression in patients with HBe Ag-negative chronic hepatitis B: A 5-year population-based cohort study. J Formosan Med Assoc. 2019;118:628-35.

Arauz J, Ramos-Tovar E, Muriel P. Coffee and the Liver, 2017:48:675-85.

Ikeda M, Maki T, Yin G, Kawate H, Adachi M, Ohnaka K, et al. Relation of coffee consumption and serum liver enzymes in Japanese men and women with reference to effect modification of alcohol use and body mass index. Scand J Clin Lab Invest. 2010;70(3):171-9.