Role of p27 in differentiating follicular adenoma and follicular carcinoma of the thyroid

Authors

  • Bimanjyoti Deuri Department of Pathology, Silchar Medical College and Hospital, Silchar, Assam, India
  • Musfika Tabassum Department of Pathology, Silchar Medical College and Hospital, Silchar, Assam, India
  • Nandinee Lahkar Department of Pathology, Silchar Medical College and Hospital, Silchar, Assam, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20210883

Keywords:

Follicular adenoma, Follicular carcinoma, Overexpression, p27, Underexpression

Abstract

Background: Thyroid neoplasms represent a broad spectrum of tumors with different biologic behaviour. Although investigations like thyroid function tests, scintigraphy and ultrasonography were routinely used for the diagnosis of thyroid nodules, they could not discriminate between benign and malignant lesions. The present study is undertaken to assess the expression of cell cycle protein such as p27kip1 (p27) in thyroid neoplasms which might be useful in predicting behaviour of various thyroid neoplasms and aid in their diagnosis. Aim of the study was to assess the expression of p27 and its role in differentiating follicular adenoma and follicular carcinoma of the thyroid.

Methods: The present study was undertaken to evaluate the expression of p27 in thyroid neoplasms with the help of immunohistochemical analysis over a period of 1 year. The study was conducted on archival blocks retrieved from the Department of Pathology, SMCH. 19 thyroid neoplasms were diagnosed (7 benign, 12 malignant) and IHC expression of p27 was studied.

Results: There was over-expression of p27 staining in case of follicular adenoma whereas malignant lesions showed underexpression on p27 staining. P27 expression was significantly different in follicular adenoma and follicular carcinoma of the thyroid.

Conclusions: The role of p27 in differentiating benign and malignant thyroid neoplasms may prove it to be a candidate marker if combined with other additional investigations like radionuclide scan or using a broad panel of IHC markers. This may provide more insight into the behaviour of tumors in a detailed manner.

References

Taneri F, Poyraz A, Salman B, Tekin E, Arkyuerek N, Bayram O, et al. Using imprint and frozen section in determining the surgical strategies for thyroid pathologies. Endocrine Regulations. 2001;35:71-4.

Wang S, Wuu J, Savas L, Patwardhan N, Khan A. The role of cell cycle regulatory proteins, cyclin D1, cyclin E, and p27 in thyroid carcinogenesis. Hum Pathol. 1998;29:1304-9.

Erickson LA, Jin L, Wollan PC, Thompson GB, van Heerden J, Lloyd RV. Expression of p27kip1 and Ki-67 in benign and malignant thyroid tumors. Mod Pathol.1998;11:169-74.

Lloyd RV, Jin L, Qian X, Kulig E. Aberrant p27kip1 expression in endocrine and other tumors. Am J Pathol. 1997;150:401-7.

Erickson LA, Yousef OM, Jin L, Lohse CM, Pankratz VS, Lloyd RV p27kip1 expression distinguishes papillary hyperplasia in Graves’ disease from papillary thyroid carcinoma. Mod Pathol. 2000;13:1014-9.

Resnick MB, Schacter P, Finkelstein Y, Kellner Y, Cohen O. Immunohistochemical analysis of p27/kip1 expression in thyroid carcinoma. Mod Pathol. 1998;11:735-9.

Liu W, Asa SL, Fantus IG, Walfish PG, Ezzat S . Vitamin D arrests thyroid carcinoma cell growth and induces p27 dephosphorylation and accumulation through PTEN/Akt-dependent and -independent pathways. Am J Pathol. 2002;160:511-9.

Kim DH, Lee HI, Nam ES, Shin HS, Sohn JH, Park CH, et al. Reduced expression of the cell-cycle inhibitor p27Kip1 is associated with progression and lymph node metastasis of gastri carcinoma. Histopathology. 2000;36:245-51.

Troncone G, Laccarino A, Russo M, Volante M, Papotti M, et al. Accumulation of p27(kip1) is associated with cyclinD3 overexpression in the oxyphilic (Hurthle cell) variant of follicular thyroid carcinoma. J Clin Pathol. 2007;60:377-81.

Golder S, Satpathy SN, Padhy RK, Panigrahi R, Ghata S. A clinicopathological study of solitary thyroid nodule. J Pharm Biomed Sci. 2015;05(03):233-7.

Modi M, Daveshwar M. Study of histopathological pattern of thyroid lesions. Int J Biomed Advance Research.2018;9(1):27-36.

Hossain MM. Surgical management of thyroid diseases: a study on 78 cases. Mymensingh Med J. 2002;11(1):628.

Raphael S, liyasul I, Mohammed AZ. Histopathological pattern of thyroid lesions in Kano, Nigeria: a 10-year retrospective review (2002-2011). Nigerian J Basic Clin Sci. 2015;12(1):55-60.

Kahmke R. Utility of intraoperative frozen sections during thyroid surgery. Int J Otolaryngol. 2013;8:75-9.

Temiz P, Akkas G, Nese N, Duman FU, Karakas C, Erhan Y. Determination of apoptosis and cell cycle modulators (p16, p21, p27, p53, BCL-2, Bax, BCL-xL, and cyclin D1) in thyroid follicular carcinoma, follicular adenoma and adenomatous nodules via a tissue microarray method. Turk J Med Sci. 2015;45:865-71.

Zhang L, Krausz T, May RM. A pilot study of Galectin-3, HBME-1, and p27 triple immunostaining pattern for diagnosis of indeterminate thyroid nodules in cytology with correlation to histology. Appl Immunohistochem Mol Morphol. 2015;23(7):481-90.

Abulkheir A, Hussein IL, Bilal D. Value of immunohistochemical expression of p27 and galectin-3 in differentiation between follicular adenoma and follicular carcinoma. Applied Immunohistochemistry Molecular Morphology. 2012;20(2):131-40.

Downloads

Published

2021-02-25

How to Cite

Deuri, B., Tabassum, M., & Lahkar, N. (2021). Role of p27 in differentiating follicular adenoma and follicular carcinoma of the thyroid. International Journal of Research in Medical Sciences, 9(3), 812–815. https://doi.org/10.18203/2320-6012.ijrms20210883

Issue

Section

Original Research Articles