The neurobiology of splitbrain crayfish

Baltazar Barrera-Mera, Juan José Granados-Romero, José Juan Vargas-Morales, Elvira Barrera-Calva, Rodrigo Banegas-Ruiz, Emilio Pérez Ortega, José Ángel Barajas-Colón, Francisco Fabian Gómez-Mendoza, Carlos Rubén Baca-Domínguez, Yuri Jiménez Caprielova, Román Alberto de la Rosa Llergo, Rodrigo Mendoza-Aceves, Ana Lilia Abundez-Pliego, Karen Uriarte-Ruiz, Mariana del Carmen Radilla-Flores, Nelson Niels Espinosa-Queb, Alejandro Ángel Corona Figueroa, Bruno Andrés Salazar-Trujillo, Alan I. Valderrama-Treviño


Background: They crayfish brain is segmented into two symmetrical hemiganglia. Normally both hemiganglia are in direct communication through a series of well-defined neural bridges that cross de midline to form reciprocal cross connections.

Methods: An original study was carried out in the Department of Physiology of the Faculty of Medicine, UNAM during the period from August 2019 to August 2020. 13 Crayfish Procambarus clarkii weighing between 1g to 30g were used. Each eyestalk of the animals was tied to a displacement transducer coupled with a polygraph so that optomotor or electrical activity was bilaterally recorded. The separation of the right from the left hemiganglia from the cerebral or supraesophageal ganglion was performed with a sagittal section, splitbrain (SB).

Results: The normal photo motor reflex in crayfish eye is measured as a gradual decrease in the ERG amplitude. During tactile stimulation, the visual activation of both eyestalks in normal crayfish leads to a highly regular bilateral activity. The regular activity can only be altered by disturbing the mechanoreceptors located in the shell surrounding the eyestalks.

Conclusions: The procedure presented in this article provides unique characteristics for the study of the nervous system such as a detailed response of the bilateral optomotor reflex.


Circadian rhythm, Electroretinography, Ganglia / physiology, Crayfish brain, Splitbrain crayfish, Circadian pacemakers

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