Comparison of direct total iron binding capacity by light MgCO3 and heavy MgCO3

Chapal Debnath; Niral G. Savaliya

doi:10.18203/2320-6012.ijrms20212239

Authors

Chapal Debnath Department of Biochemistry, New Civil Hospital, Surat, Gujarat, India
Niral G. Savaliya Department of Biochemistry, New Civil Hospital, Surat, Gujarat, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20212239

Keywords:

Iron, UIBC, TIBC, MgCO3, Calculated method, Automatic analyser

Abstract

Background: The aim of the study is focused to measure TIBC by calculated method (TIBC using the magnesium carbonate- MgCO3 adsorption), measurement of TIBC by calculatory method from automated UIBC and automated Iron measurement. Latter these two values are to be added and would give an additive result of TIBC. The experiment would be performed by using the light MgCO3 powder and the heavy MgCO3 powder and check for any differences in their respective values. Regression analysis, Bland Altman analysis or histogram analysis of TIBC results obtained by calculated methods in patient samples to find correlation between the two methods is being performed.

Methods: Samples received for analysis of tests, regardless of patient identification with sample volume serum were used for the study. After complete analysis and reporting of the sample, the leftover serum was used. Serum was kept at room temperature. Then the calculated TIBC from UIBC and the IRON were compared after doing batch calibration for UIBC and lot calibration of Iron.

Results: It was found that, this study had positive bias by the usage of the heavy MgCO3 powder. Positive bias in unsaturated iron binding capacity observed by both methods is studied and further investigated using data obtained during the experiment.

Conclusions: There is a higher yield of serum obtained with the heavy MgCO3 powder, TIBC calculated from iron and unsaturated iron binding capacity as compared to TIBC measured directly using the light MgCO3 powder.

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Author Biographies

Chapal Debnath, Department of Biochemistry, New Civil Hospital, Surat, Gujarat, India

resident , biochemistry department

Niral G. Savaliya, Department of Biochemistry, New Civil Hospital, Surat, Gujarat, India

resident , biochemistry department

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