Evaluation of in vivo anti-inflammatory activity of Ariflex tablet in comparison with diclofenac and aceclofenac tablet in carrageenan induced rat paw edema model
Keywords:Ariflex tablet, Diclofenac tablet and aceclofenac tablet, Anti-inflammatory, Carrageenan induced rat paw edema model
Background: Osteoarthritis is a major cause of pain and locomotor disability worldwide. Though various pharmacological, mechanical and surgical interventions are used, there is no known cure for OA. The present study was conducted to evaluate anti-inflammatory activity of Ariflex tablet (conceptualized and developed by Ari Healthcare Pvt. Ltd.) in comparison with diclofenac and aceclofenac tablet in carrageenan induced rat paw edema model.
Methods: Wistar rats of either sex weighing 150-180 g were taken and divided into 4 groups with 6 animals in each group i.e. group 1 (control group), group 2 (diclofenac tablet), group 3 (aceclofenac tablet) and group 4 (ariflex tablet). The study drugs were orally administered with feeding needle, 30 minutes prior to carrageenan injection. After 30 min 1% w/v of 0.05 ml carrageenan was injected subcutaneously in the rat paw. The paw was marked with ink at the level of lateral malleolus and immersed in mercury up to lateral malleolus mark. The paw volume was measured plethysmographically after injection at 30 minutes, 1 hour, 2 hour, 3 hour, 4 hour and eventually at 5 hour.
Results: All the test formulations possess statistically significant (p<0.05) anti-inflammatory activity as compared to control group. The maximum percentage inhibition for Ariflex tablet was 96.97% at the end of 5 hours. When compared to control group, statistically significant reduction of paw edema was observed. The anti-inflammatory activity of Ariflex tablet from 2 hours onwards is comparable to that of diclofenac tablet and aceclofenac tablet.Conclusions: Ariflex tablet possesses significant anti-inflammatory activity.
Hochberg MC, Altman RD, April KT, Benkhalti M, Guyatt G, McGowan J, et al. American college of rheumatology 2012 recommendations for the use of nonpharmacologic and pharmacologic therapies in osteoarthritis of the hand, hip, and knee. Arthritis Care Res. 2012;64:465-74.
Radha MS, Gangadhar MR. Prevalence of knee osteoarthritis patients in Mysore city, Karnataka. Int J Recent Sci Res. 2015;6:3316-20.
Mahajan A, Verma S, Tandon V. Osteoarthritis. J Assoc Phys India. 2005;53:634-41.
Tallheden T, Bengtsson C, Brantsing C, Carlsson L, Peterson L, Brittberg M, et al. Proliferation and differentiation potential of chondrocytes. Arthritis Res Ther.2005;7:560-8.
Scheinfeld N. A comprehensive review and evaluation of the side effects of the tumor necrosis factor alpha blockers etanercept, infliximab and adalimumab. JDermatol Treat. 2004;15:280-94.
Kongtharvonskul J, Anothaisintawee T, McEvoy M, Attia J, Woratanarat P, Thakkinstian A. Efficacy and safety of glucosamine, diacerein, and NSAIDs in osteoarthritis knee: a systematic review and network meta-analysis. Eur J Med Res. 2015;20:24.
Tascioglu F, Armagan O, Tabak Y, Corapci I, Oner C. Low power laser treatment in patients with knee osteoarthritis. Swiss Med Wkly. 2004;134:254-8.
Long L, Soeken K, Ernst E. Herbal medicines for the treatment of osteoarthritis: a systematic review. Rheumatology 2001;40:779-93.
Sawarkar G, Sawarkar P. Prevention and management of osteoarthritis. Int J Res Ayurved Pharm. 2013;4: 454-8.
Akhtar B, Mahto RR, Dave AR, Shukla VD. Clinical study on Sandhigata Vata w.s.r. to osteoarthritis and its management by Panchatikta Ghrita Guggulu. Ayurved.2010;31:53-7.
Cao H, Yu R, Choi Y, Zhang H, Xiang W, Lee D, et al. Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation. Pharmacol Res. 2010;61:519-24.
Shishodia S, Aggarwal B. Guggulsterone inhibits NF- B and I B kinaseactivation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis. J Biol Chem. 2004;279:47148-58.
Srivastava P. Chemical standardization of some anti-inflammatoryRasayana plants used in Indian system of medicine. Afr J Tradit Complement Altern Med. 2007;4(3):319-37.
Gacche R, Shaikh R, Pund M, Deshmukh R. Cyclooxygenase inhibitory, cytotoxicity and free radical scavenging activities of selected medicinal plants used in Indian traditional medicine. Pharmacog. 2011;3:57-64.
Chattopadhyay P, Hazarika S, Dhiman S, Upadhyay A, Pandey A, Karmakar S, et al. Vitex negundo inhibits cyclooxygenase-2 inflammatory cytokine-mediated inflammation on carrageenan-induced rat hind paw edema. Pharmacogn Res. 2012;4:134-7.
Philip S, Tom G, Vasumathi AV. Evaluation of the anti-inflammatory activity of Tinospora cordifolia (willd.) miers chloroform extract - a preclinical study. J Pharm Pharmacol. 2018;70:1113-25.
Luseba D, Elgorashi EE, Ntloedibe DT, Staden JV. Antibacterial, anti-inflammatory and mutagenic effects of some medicinal plants used in South Africa for the treatment of wounds and retained placenta in livestock. S Af J Bot. 2007;73:378-83.
Richard B van Breemen, Tao Y, Li W. Cyclooxygenase-2 inhibitors in ginger (Zingiber officinale). Fitoterapia. 2011;82:38-43.
Samson DJ, Grant MD, Ratko TA, Bonnell CJ, Ziegler KM, Aronson N. Treatment of primary and secondary osteoarthritis of the knee. Evid Rep Technol Assess. 2007;1-157.
Dhingra M, Nain P, Nain J, Malik M. Hepatotoxicity v/s hepatoprotective agents- a pharmacological review. Int Res J Pharm. 2011;2:31-7.
Bjorkman DJ. Current status of nonsteroidal anti-inflammatory drug (NSAID) use in the United States: risk factors and frequency of complications. Am J Med. 1999;107:3-8.
Aletaha D, Smolen JS. Laboratory testing in rheumatoid arthritis patients taking disease-modifying antirheumatic drugs: clinical evaluation and cost analysis. Arthritis Rheum. 2002;47:181-8.
CaoH, Yu R, Choi Y, Zhang H, Xiang W, Lee D, et al. Discovery of cyclooxygenase inhibitors from medicinal plants used to treat inflammation. Pharmacol Res. 2010;61:519-24.
Alam M, Khan H, Samiullah L, Siddique KM. A review on phytochemical and pharmacological studies of Kundur (Boswellia serrata roxb ex colebr.)-a Unani drug. J App Pharm Sci. 2012;2:148-56.
Srivastava KC, Mustafa T. Ginger (Zingiber officinale) in rheumatism and musculoskeletal disorders. Med Hypotheses. 1992;39:342-8.
Ojewole JA. Analgesic, Antiinflammatory and Hypoglycaemic Effects of ethanol extract of Zingiber officinale (roscoe) rhizomes (Zingiberaceae) in mice and rats. Phytother Res. 2006;20:764-72.
Sharma JN, Sharma JN. Comparison of the anti-inflammatory activity of Commiphora mukul (an indigenous drug with those of phenylbutazone and ibuprofen in experimental arthritis induced by mycobacterial adjuvant. Arzneimittel-Forschung. 1977;27:1455-57.
Singh BB, Mishra LC, Vinjamury SP, Aquilina N. The effectiveness of Commiphora mukul for osteoarthritis of the knee: an outcomes study. Altern Ther Health Med. 2003;9:74-9.
Arya D, Patni V. Microprogagation of Pluchea lancelata (oliver & Hiren,)- a potent antiarthritic medicinal herb. Int J Med Arom Plants. 2013;3:55-60.
Sumantran VN, Kulkarni A, BoddulS, ChinchwadeT, KoppikarSJ, Harsulkar A, et al. Chondroprotective potential of root extracts of Withania somnifera in osteoarthritis. J Biosci. 2007;32:299-307.
Rasool M, Varalakshmi P. Immunomodulatory role of withania somnifera root powder on experimental induced inflammation: an in vivo and in vitro study.Vasc Pharmacol. 2006;44:406-10.
Singh GB, Bani S, Singh S. Toxicity and safety evaluation of boswellic acids. Phytomedicine 1996;3:87-90.
Vishwanathan AS, Basavaraju R. A review on Vitex negundo L. - a medicinally important plant. Eur J Behav Sci. 2010;3:30-42.
Dharmasiri MG, Jayakody A, Galhena G, Liyanage SS. Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo. J Ethnopharmacol. 2003;87:199-206.