A study to assess the feasibility of using hemolysis index to predict the corrected potassium in a hemolysed sample

Authors

  • Biswadeep Choudhury Department of Biochemistry, Silchar Medical College, Silchar, Assam, India
  • Nabiha Mayanaz Karim Department of Biochemistry, Silchar Medical College, Silchar, Assam, India
  • Manidip Chakraborty Department of Biochemistry, Silchar Medical College, Silchar, Assam, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20212525

Keywords:

Hemolysis, HI, Estimated potassium, Hyperkalemia

Abstract

Background: Potassium is one of the most commonly affected analytes in a hemolysed sample. Many formulae have been devised to predict the actual potassium in a hemolysed sample. This study was performed to compare the predicted potassium value in a hemolysed sample to that of potassium value in a non-hemolysed sample of the same patient.

Methods: The hemolytic index (HI) derived equation from the paper by Dimeski et al was used to calculate potassium value in this study. A total of 99 paired samples were evaluated where the first sample in a pair was the hemolysed one and the other sample was a non-hemolysed one.

Results: This study found that the potassium value in a sample and its respective HI have weak positive correlation. However, there was a statistically significant strong positive correlation between the estimated potassium of hemolysed sample to that of the potassium in the non-hemolysed sample.

Conclusions: Hence, we conclude that it is feasible to use HI-derived equation to predict potassium in a hemolysed sample to avoid repetition of each sample.

References

Carraro P, Plebani M. Errors in a stat laboratory: types and frequencies 10 years later. Clin Chem. 2007;53:1338-42.

Lippi G, Blanckaert N, Bonini P. Haemolysis: an overview of the leading cause of unsuitable specimens in clinical laboratories. Clin Chem Lab Med. 2008;46:764-72.

Sodi R, Darn SM, Davison AS, Stott A, Shenkin A. Mechanism of interference by haemolysis in the cardiac troponin T immunoassay. Ann Clin Biochem. 2006;43:49-56.

Clinical and Laboratory Standards Institute. Hemolysis, icterus, and lipemia/turbidity indices as indicators of interference in clinical laboratory analysis; Approved guideline. CLSI document C56-A. Wayne, PA: Clinical and Laboratory Standards Institute. 2012.

Dolci A, Panteghini M. Harmonization of automated hemolysis index assessment and use: Is it possible? Clinica Chimica Acta. 2014;432:38-43.

Dimeski G, Clague AE, Hickman PE. Correction and reporting of potassium results in haemolysed samples. Ann Clin Biochem. 2005;42(Pt 2):119-23.

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Published

2021-06-25

How to Cite

Choudhury, B., Karim, N. M., & Chakraborty, M. (2021). A study to assess the feasibility of using hemolysis index to predict the corrected potassium in a hemolysed sample. International Journal of Research in Medical Sciences, 9(7), 2015–2018. https://doi.org/10.18203/2320-6012.ijrms20212525

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Section

Original Research Articles