@article{Kanna B._Kumar G._M._Easow_2017, title={Isolation and identification of candida species from various clinical samples in a tertiary care hospital}, volume={5}, url={https://www.msjonline.org/index.php/ijrms/article/view/3253}, DOI={10.18203/2320-6012.ijrms20173554}, abstractNote={<p class="abstract"><strong>Background:</strong> <em>Candida spp </em>is a member of the normal flora of the skin, mucous membrane and gastrointestinal tract. They are endogenous opportunists which cause secondary infection in individuals with underlying immunocompromised conditions. Candidiasis is a common fungal disease in humans. An increase in the prevalence of non-albicans species has been noted during the last decades because of increasing use of azoles. This study aims to <em>Spectate Candida </em>using chromogenic medium.</p><p class="abstract"><strong>Methods:</strong> A total of 50 <em>Candida</em> isolates from various clinical samples were included in the study. These isolates were subjected to gram’s stain, germ tube test and inoculation on commercially available CHROM agar (HiMedia India).</p><p class="abstract"><strong>Results:</strong> In current study majority of isolates were from high vaginal swab (34%) followed by sputum (28%), urine (18%), pus from surgical sites and others constituted to 20%. <em>Candida albicans</em> (51%) was the most common <em>candida</em> species, followed by <em>C. tropicalis</em> (25%),<em> C. krusei</em> (16%), <em>C. glabrata</em> (6%) and <em>C. dubliniensis </em>(1%).</p><p><strong>Conclusions:</strong> Along with<em> Candida albicans</em>, non-albicans<em> candida spp</em> like <em>C. tropicalis</em>, <em>C. krusei</em>, <em>C. glabrata</em>, and <em>C. dubliniensis</em> are increasingly being isolated from clinical samples. CHROM agar is a simple, rapid and inexpensive method for identification of such species. Characterization to species level helps to identify species which might be intrinsically resistant to commonly used antifungal agents.</p>}, number={8}, journal={International Journal of Research in Medical Sciences}, author={Kanna B., Vignesh and Kumar G., Amar and M., Swapna and Easow, Joshy M.}, year={2017}, month={Jul.}, pages={3520–3522} }